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Objective To investigate the expressions of FSH receptor mRNA and protein in ovary tissue in rats with letrozole-induced polycystic ovary syndrome(PCOS),and to provide experimental data for the model application. Methods Forty rats were randomly divided into two groups(n=20),in PCOS model group letrozole was administered once daily during 21 d,and in control group without any treatment.The gonadal hormone concentrations in serum were determined by radioimmunoassay,the histologic changes in ovaries were observed by HE staining,the expression of FSH receptor gene in ovary tissue was detected by realtime -PCR,Western blotting and immunohistochemistry.Results Compared with control group,estradiol(E2) and progesterone in model group showed a considerable reduction(P0.05).Compared with control group,the ovaries from model group showed high incidence of subcapsular ovarian cyst and capsular thickening and decreased number of corpora lutea.The expressions of FSH receptor mRNA and protein were significantly higher in model group than those in control group(P
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the preventive effect of antioxidant and calcium channel blockade on testicular fibrosis in rats, and to explore the ideal drug for preventing it.</p><p><b>METHODS</b>Eighty Wistar rats were divided into a control group (Group A, n = 10), a treatment group (Group B, n = 57) and a testicular fibrosis model group (Group C, n = 13). And the treatment group was further divided into a higher dosage group (Group a, n = 20), a medium dosage group (Group b, n =20) and a lower dosage group (Group c, n = 17). Testicular fibrosis was duplicated with altered Wang Tao's method. From the second day of the first immunization, the higher dosage group was given antioxidant vitamins 90 mg/(kg x d) and verapamil 50 mg/(kg x d), the medium dosage group antioxidant vitamins 90 mg/(kg x d) and verapamil 25 mg/(kg x d), and the lower dosage group antioxidant vitamins 90 mg/(kg x d) and verapamil 12.5 mg/(kg x d), all for 150 days. The control and the model groups received no treatment. The sperm count, sperm deformity rate, testis length and seminiferous tubule intradiameter were measured, and the changes of the testis interstitial substance and spermatogenic cells were observed by light microscope and transmission electron microscope.</p><p><b>RESULTS</b>Testicular fibrosis was significantly prevented by the higher- and medium-dosage treatment in the rats. In the higher dosage group, the intradiameter of the seminiferous tubules and the thickness of the limiting membrane were almost the same as in the control. In the lower dosage group the thickness of the limiting membrane was thicker and the damage to the spermatogenic cells was heavier than in the control, but the pathological changes of the testis structure was lighter than in the model group, in which Hyperplasia and fibroblast increase in the interstitial substance were significant, interstitial mast cells and peritubular mast cells increased, the thickness of the limiting membrane of the seminiferous tubules seriously thickened, and the damage to the spermatogenic cells was severe.</p><p><b>CONCLUSION</b>Testicular fibrosis in rats can be significantly prevented by antioxidant and calcium channel blockade.</p>