ABSTRACT
To investigate the changes in the expression of G proteins ? subunit mRNA in non functioning thyroid adenoma (NFTA) and thyroid papillary cancer (TPC). Eleven thyroid specimens were obtained during surgery, 6 of which from patients with NFTA and 5 from patients with TPC. The expression levels of stimulating and inhibiting G protein ? subunit mRNAs were determined by reverse transcription polymerase chain reaction (RT PCR). The expression levels of Gsa mRNA in TPCs were significantly higher than those in normal thyroids and NFTAs( P
ABSTRACT
Objective:To investigate the role of saturated free fatty acids (SFFAs) in the pathogenesis of atherosclerosis by determining the effects of FFAs on diacylglycerol (DAG) synthesis and protein kinase C (PKC) activity in cultured bovine aortic smooth muscle cells. Methods:Bovine aortic smooth muscle cells, cultured in vitro, were treated with 200 ?mol/L SFFAs (including palmitate and stearate) and unsaturated FFAs (including oleate and arachidonate) separately for 24 h. Then the cells were lysed and the lipids were treated with lysis buffer and separation buffer. DAG level and PKC activity were calculated through measuring the radioactivity of 32P-PA by liquid scintillation counting (1 cpm=6.17?108 Bq). Results:Incubation of the cells with saturated FFAs(palmitate or stearate, 200 ?mol/L, for 24 h) induced significant increase in DAG concentrations (927.2 and 1 533.3 pmol/106 cells by palmitate and stearate, respectively) compared with that of the control cells (190 pmol/106 cells,P
ABSTRACT
To evaluate the prevalence and incidence of coronary heart disease (CHD) and associated risk factors with CHD in the elderly, a survey was conducted among old subjects aged 60 to 90 who had health examination in PLA General Hospital from 1996 to 1997. A cohort population without CHD had been followed up until 2000, and the incidence of CHD was calculated as the baseline. The incidence of CHD was calculated by person year method. The risk factors of incidence in CHD of different groups were analyzed with multiple logistic regression at the end of four observation years. The incidences of CHD and myocardial infarction (MI) of IGT and DM group were significantly higher than that of NGT group( P