ABSTRACT
<p><b>OBJECTIVE</b>To measure the immunogenicity of a synthetic peptide of glucosyltransferase (GTF) for designing synthetic peptide-based vaccine of dental caries.</p><p><b>METHODS</b>A fusion 27-mer peptide, containing the conserved regions within catalytic and glucan-binding domains of GTF, was synthesized. Serum antibodies to the synthetic peptide were determined by ELISA method. Inhibitions of both GTF activity and S. mutans adherence were detected for the functions of antisera.</p><p><b>RESULTS</b>The sequence of fusion peptide vaccine was ANDVDNSNPVVQAEQLYFRANGVQVKG. The spleen weights of immunized mice were heavier than the control ones. Specific antibodies were effectually elicited. The immune sera not only inhibited GTF enzymatic activity but also inhibited the vitro adherence of S. mutans.</p><p><b>CONCLUSIONS</b>The peptide vaccine which involves antibody-mediated inhibition of the catalytic and the glucan-binding activities of GTF may be valuable for controlling the dental caries.</p>
Subject(s)
Animals , Male , Mice , Rats , Amino Acid Sequence , Antibodies , Blood , Bacterial Adhesion , Dose-Response Relationship, Drug , Glucosyltransferases , Genetics , Allergy and Immunology , Immune Sera , Pharmacology , Immunization , Mice, Inbred BALB C , Molecular Sequence Data , Oligopeptides , Allergy and Immunology , Streptococcus mutans , Physiology , Vaccines, Subunit , Allergy and ImmunologyABSTRACT
Objective The microcapsules were prepared by using chitosan and sodium alginate as wall materials,and the controlled-release microcapsulas of interferon tau were hoped to be develop an oral interferon preparation.Methods The microcapsules were prepared by using syringe hand-made drop.In the process of dropping,speed and distance were the major factors which influence the form of microcapsules.Results Chitosanalginate microcapsules which was prepared simple and fast had high encapsulation rate and extended-release effect in intestine.Conclusion Chitosan-alginate microcapsules have the potential to be used to prepare interferon tau and other protein medicines.