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AIM: To investigate the effects of bone morphogenetic protein 7 ( BMP-7 ) on the expression of transcription factor E2A and inhibitor of differentiation 2 (Id2) in the renal tubule epithelial cells(NRK-52E)exposed to high glucose, and to explore its possible mechanism of improving renal tubular fibrosis induced by high glucose.METH-ODS:The NRK-52E cells were divided into control group, high glucose (HG) group and high glucose with different doses of BMP-7 (10μg/L and 20μg/L) group.The cells in HG group and BMP-7 group were cultured for 12 h, 24 h and 48 h. The protein expression of Id2, E2A, E-cadherin,α-smooth muscle actin (α-SMA) and collagen-I was detected by Western blot.In addition, the mRNA expression of Id2 was detected by real-time PCR.RESULTS:Compared with control group, the mRNA and protein levels of Id2 and the protein level of E-cadherin were down-regulated, while the protein levels of E2A,α-SMA and collagen-I were up-regulated in HG group (P<0.05).Compared with HG group, the mRNA and pro-tein levels of Id2 and the protein level of E-cadherin were significantly up-regulated, while the protein expression of E2A,α-SMA and collagen-I was significantly down-regulated in 20 μg/L BMP-7 group ( P<0.05 ) .The correlation analysis showed that the Id2 protein level was negatively correlated with the E2A protein level (P<0.05).CONCLUSION:BMP-7 may intercept the process of renal tubule fibrosis induced by high glucose via promoting the expression of Id2 and inhibi-ting the expression of E2A at protein level.
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AIM:To investigate the effects of proteasome inhibitor MG 132 on the expression of SnoN in renal tubule epithelial cells incubated in high glucose , and to explore the possible mechanism and function that MG 132 reduces or slows down renal tubular interstitial injury after incubated in high glucose .METHODS:The NRK-52E cells were divid-ed into normal control group (NG), high glucose group (HG) and high glucose plus pretreatment with different doses of MG132 group (HG+MG132).The immunofluorescence staining was used to detect the protein expression of E-cadherin and α-smooth muscle actin (α-SMA) in NRK-52E cells under different conditions .The relative protein expression levels of SnoN, Smad ubiquitination regulatory factor 2 (Smurf2), Arkadia, E-cadherin, α-SMA and collagen type Ⅰ(Col-Ⅰ) were detected by Western blotting .RESULTS:Compared with NG group , the expression of E-cadherin and SnoN was de-creased (P<0.05), while the expression of α-SMA, Col-Ⅰ, Smurf2 and Arkadia was increased (P<0.05).Compared with HG group, the protein expression of SnoN and E-cadherin was significantly up-regulated in HG+MG132 group ( P<0.05 ) , and the protein expression of α-SMA and Col-Ⅰwas significantly down-regulated in a dose-depended manner ( P<0.05).However, no effect on the protein expression of Smurf2 and Arkadia was observed.CONCLUSION: MG132 in-hibits the degradation of SnoN protein induced by high glucose , thus reducing the renal fibrosis .
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Objective To investigate the clinical and laboratory characteristics of acute myeloid leukemia (AML) with t (16;21)(p11;q22) translocation.Methods Two patients diagnosed by morphology,cytochemical stain,immunology,cytogenetics and genetic testing.Similarities and differences of clinical characteristics and laboratory examination were analysed,along with a review of the literatures.Results According to the FAB classification,one patient was M4 and the other one was M1.The cytogenetic aberrations were 46,XY,t(16;21)(p11;q22)[16]/47,XY,t(16;21)(p11;q22),+21[4] of ease 1 and 46,XX,t(16;21)(p11;q22)[20] of case 2.Cytophagocytosis and CD56 antigen expression were found in both cases.The prognosis was poor in both cases.Conclusions AML with t(16;21)(p11;q22) is a specific type,which has unusual characteristics of morphology,immunology,cytogenetics,clinical feature.The prognosis of the patients is poor,so stem-cell transplantation maybe the only and the first choice of treatment.
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Chief Physician WANG Hui-reng is one of the second batch of national prestigious TCM experts. He has been engaged in medical services over 40 years and is adept in preventing and treating the chronic obstructive pulmonary disease (COPD) under the direction of prophylactic treatment, which emphasizes the control of risky factors of COPD, striving for protecting the healthy body, preventing the transmission of the disease and the recurrence after healing.