ABSTRACT
Objective:To investigate the animal model of guinea pig chronic heart failure and compensatory hypertrophy. Methods:Thirteen chronic heart failure models were made by placing a constricting band around the ascending aorta of guinea pigs,raised in the same house of 10 normal guinea pigs. The contrasting indexes in 6 weeks include: clinical features, hemodynamics, the mass ratio of ventricular over body, the width of left ventriculat hypertrophy. Results:One model died, 4 models without dyspnea showed increasing in left ventricular pressure and the mass ratio of ventricular over body, whereas the left ventricular end diastolic pressure showecl no significant different. Eight models with dyspnea showed striking increasing in left ventricular pressure and left ventricular end diastolic pressure, as well as the mass ratio of ventricular over body and the width of left ventricular hypertrophy. Conclusion:The animal model of guinea pig chronic heart failure and compensatory hypertrophy may be formed by constricting the ascending aortas of guinea pigs for 6 weeks.
ABSTRACT
Whether InfusaSleeve(IS) catheter can deliver antisense oligodeoxynucleotide (ODNs) following arterial denuation is unknown. We evaluate the feasibility of local endoluminal delivery of C-myc ODNs to the site of arterial denudation by using IS catheter and to determine the biological importance of these effects. IS catheter was introduced into right side of iliac artery of 21 rabbits after angioplasty of iliac artery. Animals were randomized to the control group (n＝6) receiving saline injection and the treated group receiving c-myc antisense (n＝15, 1 mg ODNs per vessel). In two weeks and 40 days following the operation, angiography was performed. Morphometric analyses were carried out in balloon-denuded iliac arteries. The expression of c-myc protein was detected by using a mouse monoclonal antibody to c-myc. Morphometric analyses carried out at 40 days after transcatheter c-myc antisense oligomer administration. The results showed that maximal neointimal area was reduced from 7.66±3.7(×105 μm2) in the control group (n＝6) to 4.04±1.02(×105 μm2) in the antisense treated group (n＝6, P＜0.05). These changes in vascular remodeling following denuding injury resulted in an increase in residual luman from 20～50% in the control group to 70～90% in the antisense-treated group. C-myc protein expression was virtually undetectable at baseline in locally ODNs-delivered arteries and detectable in control denuded arteries. The results show that: ①Single IS transcatheter administration allowed endoluminal delivery of ODNs to the site of arterial injury; ② c-myc antisense oligomer reduced the formation of neointime in denuded arteries, implying a therapeutic potential of this approach.
ABSTRACT
This paper reports the postoperative managenment of 58 cases who had various types of congenital heart disease and received artificial valves due to rheumatic heart disease in our institute from 1982 to 1984. Vaso-active agents were administered with the monitoring of the cardiac function. According to the hemodynamic parameters and the criterion for clinical typing, the vaso-active agents therapy can provide a precise prognosis and the therapy is of certain value in cardiac surgery.