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Aim To establish non-alcoholic fatty liver disease mouse model and study different kinds of lymphocytes in C57BL/6J mouse model. Methods SPF male C57BL/6J mice were randomly divided into control group ( normal diet ) and model group( normal diet with high fat diet by gavage) . Models of non-alcoholic fatty liver disease were established. At 12th and 16th weeks, body weight, liver index, serum TC, TG, HDL, LDL, ALT, AST were measured. Pathological examination of fat deposition in liver was performed. Flow cytometry was used to assay the percentage of natural killer cells, T helper cells, natu-ral killer T cells and IL4 +NKT cells in mouse liver. Results Liver index, serum TC, LDL, ALT, AST were significantly higher in model group(P<0.05) after 16 weeks. Pathological sections showed that liver fat deposition in model group was quite severe and large lipid droplets spread through the mouse liver. The percentage of natural killer T cells increased significantly( P<0.05 ) and the percentage of IL4 +NKT cells increased even more obviously(P<0.01). Conclusions C57BL/6J mice fed with normal diet and high fat diet by gavage can form a good non-alcoholic fatty liver disease mouse model. In this model, the number and activity of natural killer T cells are significantly changed, and natural killer T cells may be the new target of the mechanism and drug treatment of nonalcoholic fatty liver dis-ease.
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Objective To discover the effect of partial splenic embolization on the immune function of cirrhotic patients with hypersplenism. Methods Patients involved in the study were enrolled and divided into three groups, including control group, experimental group, and complication group. Numbers of CD3
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OBJECTIVE@#To discover the effect of partial splenic embolization on the immune function of cirrhotic patients with hypersplenism.@*METHODS@#Patients involved in the study were enrolled and divided into three groups, including control group, experimental group, and complication group. Numbers of CD3(+), CD4(+) and CD8(+) T cells and CD4(+)CD25(+)CDl27(low/-) Treg cells in the peripheral blood of patients before surgery, 1 month, 6 months, 1 year, and 2 years after surgery were analyzed by fluorescence active cell sorting (FACS). Contents of immunoglobulins (IgA, IgG and IgM) were analyzed by auto immunoassay analyzer.@*RESULTS@#In the peripheral blood of patients from experimental group, numbers of CD3(+), CD4(+) and CD8(+) T cells initially declined, but afterwards increased to normal level; in the peripheral blood of patients from complication group, CD3(+) and CD8(+) T cells showed the same trend, but the number of CD4(+) T cells was below normal level at all detection times. Furthermore, CD3(+), CD4(+) and CD8(+) T cells in the peripheral blood of patients from complication group were initially less than those in experimental group, and afterwards were comparable between two groups. In patients from both experimental group and complication group, the number of CD4(+) CD25(+) CDl27(low/-)Treg cells increased 1 month and 6 months after surgery, and gradually restored to normal level. CD4(+)CD25(+)CDl27(low/-) Treg cell counts in patients from complication group were initially more than those in patients from experimental group 1 month and 6 months after surgery, but then they were comparable. Furthermore, contents of immunoglobulins (IgA, IgG and IgM) were comparable in three groups at all detection times.@*CONCLUSION@#Partial splenic embolization influenced the immune function of cirrhotic patients with hypersplenism in the short term but the immune function could afterwards gradually restore to normal. Our results implicated that measures that prevent infection and improve immune function were necessary in early stage after undergoing PSE in order to reduce complications.
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<p><b>AIM</b>To investigate the role of three K+ channels-delayed rectifier K+ channel (Kv), large-conductance Ca(2+)-activated K+ channel (BK(Ca)) and ATP-sensitive K+ channel (K(ATP)) in the regulation of the resting and contracting tone of rat bronchial smooth muscle (BSM).</p><p><b>METHODS</b>By measuring the isometric tone of bronchial strips in vitro, the regulating effects of the three K+ channels on the tone of rat BSM were observed.</p><p><b>RESULTS</b>(1) Kv blocker 4-aminopyridine (4-AP) caused concentration dependent contraction in resting bronchial strips, but BK(Ca) blocker tetraethylammonium (TEA) and K(ATP) blocker glibenclamide (Glib) had no such effects. (2) The bronchial epithelium had no effects on the contraction induced by 4-AP, but nifedipine, a Ca2+ channel blocker, significantly suppressed it. (3) Before or after treatment with 0.1 mmol/L histamine or 50 mmol/L KCl, administration of TEA (1 or 5 mmol/L) or 0.1 mmol/L 4-AP could significantly increase the contraction induced by histamine and KCl in bronchial strips. But Glib (10 micromol/L) had no effect on it.</p><p><b>CONCLUSION</b>Not BK(Ca) and K(ATP) but Kv participated in the regulation of the resting tone in rat BSM. The closure of BK(Ca) or Kv increased the contracting tone induced by histamine or KCl in rat BSM in vitro, but K(ATP) had no such effect on it.</p>