Effects of renal denervation on blood-pressure response to hemorrhagic shock in spontaneously hypertensive rats / 中华创伤杂志(英文版)

<p><b>PURPOSE</b>Renal denervation (RD) has been demonstrated to be an effective approach to reduce blood pressure for those with resistant hypertension. Yet, we aimed to explore the effect and possible mechanism of RD on blood-pressure response to hemorrhagic shock in spontaneously hypertensive rats.</p><p><b>METHODS</b>A total of 48 male spontaneously hypertensive rats were randomized to three groups: study group, sham-operation group and control group. RD was achieved by cutting off renal nerves and swabbing phenol on it. Ten weeks after RD, 8 rats in each group were sacrificed to collect the kidney and heart tissues. The remaining rats were subjected to an operation to induce hemorrhagic shock which would lead to 40% loss of total blood volume, and observed for 120 min. The serum concentration of norepinephrine was measured before and three weeks after RD.</p><p><b>RESULTS</b>The blood-pressure and norepinephrine levels were reduced significantly after RD (p < 0.05). Systolic blood pressure and diastolic blood pressure of the surgery group were higher than those in the sham and control groups at 15, 30 and 45 min after hemorrhagic shock (p < 0.05), while no significant difference was observed at 60, 90 and 120 min (p > 0.05). Additionally, the beta-1 adrenergic receptor (β1-AR) in the study group was significantly higher than those in the other two groups (p < 0.05) after hemorrhagic shock.</p><p><b>CONCLUSION</b>This study demonstrated that RD could to some extent improve blood-pressure response to hemorrhagic shock in an established model of severe hemorrhagic shock in spontaneously hypertensive rats. The mechanism might be associated with up-regulation of β1-AR.</p>

Patterns of hepatic cells death during hepatic ischemia/reperfusion injury in cirrhotic rats / 中国医学科学院学报

<p><b>OBJECTIVE</b>To research on the main pattern of hepatic cells death during hepatic ischemia/ reperfusion (I/R) injury in cirrhotic rat.</p><p><b>METHODS</b>Cirrhotic rat model was established by carbon tetrachloride replication. These rats were randomly divided into sham operation group and I/R group. In the I/R group, 70% i/R injury model was established and then the liver samples were taken 0, 1, 6, 24, and 48 hours after reperfusion. Serum alanine aminotransferase (ALT)/aspartate aminotransferase (AST) levels, Na+ - K+ ATPase, and Ca2+ ATPase were compared. the percentage of apoptotic/oncotic hepatic cells was measured with flow cytometry, and the changes in hepatic cellular structures were observed under transmission electron microscope.</p><p><b>RESULTS</b>Compared with the sham operation group, the levels of serum AST and ALT significantly increased in the I/R group (P < 0.05), reaching their peak levels at the 6th hour. The activities of Na+ - K+ ATPase and Ca2+ ATPase dramatically decreased one hour after reperfusion and then gradually recovered (P < 0.05). Hepatic cells mainly suffered oncosis at the early stage after reperfusion (within 6 hours); at the late stage (around 24 hours after reperfusion), apoptosis became the main death pattern.</p><p><b>CONCLUSION</b>Oncosis is the main pattern of hepatic cells death during I/R injury in cirrhotic rat, and the severity of hepatic injury correlates with the oncosis.</p>

Effect of Balance Training on Cerebral Cortex and Balance Function of Mouse / 中国康复理论与实践

@#Objective To observe the effect of balance training on cerebral cortex and balance function of mouse.Methods Forty-five C57BL/6J female mice were randomly divided into the control group, two-week training group and four-week training group with 15 animals in each group. An new model of balance function training was copied. The balance function, brain index, and the content of malondialdehyde (MDA) and activity of superoxide dismutase (SOD) in the mouse cerebral cortex were tested after training.Results The total time passing balance beam of the four-week training group significantly shorten compared with the control group and two-week training group ( P<0.01). Compared with the control group, the brain index of two-week training group increased ( P<0.05), and four-week training group also increased significantly ( P<0.01). Compared with the control group, the SOD activity of cerebral cortex significantly increased (P<0.01), and MDA decreased ( P<0.05) in the four-week training group.Conclusion This new balance training can improve balance function, increase the brain index and decrease lipid peroxidation level in the cerebral cortex of the mice.