Study on intraspecific genetic diversity in different plant populations of Pogostemon cabli / 中国中药杂志

<p><b>OBJECTIVE</b>To study the genetic polymorphism and intraspecific genetic differentiation of different populations of Pogostemon cablin, and find out the effective method to distinguish DNA fingerprint of different populations of P. cablin.</p><p><b>METHOD</b>Five plant populations of P. cablin were analyzed by RAPD markers. PopGen 32 software for clustering analysis and calculating. Fourteen of the 80 random primers were tested to possess the stronger detecting effect of polymorphous character.</p><p><b>RESULT</b>A total of 84 bands was amplified by the 10 primers, among them 17 bands were monomorphic. 67 of them were polymorphic. The results indicated that the genetic variations existed within the different plant populations of the same species.</p><p><b>CONCLUSION</b>It is feasible by RAPD technique with specifically primer to analyze the genetic diversity and identify 5 plant populations of P. cablin. RAPD technique has provided a new path for identification and classification of P. cablin genetic germplasm.</p>

Investigation on the influential factors of the volatile oil and main constituent content in Pogostemon cablin / 中国中药杂志

<p><b>OBJECTIVE</b>To provide scientific basis for quality evaluation by investigating the influential factors of the volatile oil and main constituent content in Pogostemon cablin.</p><p><b>METHOD</b>Several experiments were carried out to test different habitats, collection periods, processing methods, the level of spreading manure and using agricultural chemical with the volatile oil assay of pharmacopoeia and GC-MS method.</p><p><b>RESULT</b>The effect of different habitats, collection periods, processing methods on volatile oil and main constituent content was shown significantly.</p><p><b>CONCLUSION</b>Such factors as different habitats, collection periods, processing methods should be taken into account when the quality standard of medicinal materials was made.</p>