ABSTRACT
In the study, fluorescent enzyme-linked immnoabsorbent assay for detection of Staphylococcus aureus was established with IgG from pig as capture antibody and quantum dot nanobeads (QBs) labeled vancomycin (QB-Vans) as testing antibody. Quantum dot of about 100 nm partical size nanobeads were prepared and linked with vancomycin. The optimum concentrations of salt ions were 0.01 mol/L, and the optimum pH was 6.0. Under the optimum conditions, the detection sensitivity for S. aureus was 10⁴ CFU/mL, and there was no cross-reaction with other pathogenic bacteria. Thus, the method could be used for rapid screening of S. aureus, for the clinical monitoring and foodborne pathogens detection.
ABSTRACT
OBJECTIVE:To compare the five kinds of Sini powder prepared in accordance with the standards for antiquity decoction, Japanese standard decoction, traditional recipe, hospital medicine decocting or traditional Chinese medicine granules so as to provide theoretic bases for the experimental study of the decoction and the standardization of its clinical practice. METHODS: HPLC was applied with the comprehensive scores on the contents and the extractum yield of peoniflorin, naringin, ammonium glycyrrhizinate salt and saikoside a as index. RESULTS: The Sini powder decoction prepared in accordance with the traditional recipe showed the highest comprehensive scores. CONCLUSION: The traditional recipe decoction method was established as the preparation technology for the standard Sini powder decoction.