ABSTRACT
Objective To evaluate the risk of in-stent restenosis (ISR) after coronary artery stenting according to features of plaques on coronary artery CTA.Methods Totally 166 patients underwent coronary artery CTA before and 6-18 months after stent placement.Then the patients were divided into ISR group (n=16) and non-ISR group (n=150).The stenosis degree and plaque features were observed and compared between the two groups.Multivariate Logistic regression analysis was performed to evaluate ISR risk factors,and ROC curve was used to evaluate the diagnostic efficacy of plaques features in prejudgement of ISR.Results The lesion length,the ratio of noncalcified plaque,spotty calcium,positive remodeling and positive remodeling index in ISR group were higher than those in non-ISR group (all P ＜ 0.05).Multivariate Logistic regression analysis showed that noncalcified plaque (B=1.89,odd rate [OR] =6.63,P=0.01),spotty calcium (B=1.28,OR=3.59,P=0.01),positive remodeling (B=2.17,OR=8.71,P＜0.01) and lesion length (B=0.05,OR=1.05,P=0.04) were the risk factors of ISR.The area under the ROC curve (AUC) of lesion length and positive remodeling index for diagnosing ISR was 0.70 and 0.82 (both P＜ 0.01),respectively.The AUC of the combination of above plaque features for prejudgement of ISR was 0.87 (P＜0.01).Conclusion Lesion length,positive remodeling,noncalcified plaque and spotty calcium can be used to evaluate ISR after coronary artery stent placement.
ABSTRACT
Objective To report our first clinical experience with a novel modified culotte technique for the treatment of true coronary bifurcation lesions. Methods The novel modified culotte technique (the mono-ring culotte) stenting was done in which the side branch (SB) stent was deployed firstly followed by ex vivo wiring of a most proximal cell of SB stent with the hard end of main branch (MB) wire. Secondly, the MB stent was deployed through the most proximal cell of SB stent. The procedure was ended with kissing balloon dilation. From June 2014 to March 2015, 15 patients with true coronary bifurcation lesion were treated with mono-ring culotte stenting in our center. Results The procedures were successful in all cases without procedural complication and in-hospital major adverse cardiovascular events. The procedural time was (34. 3 ± 9. 6) min, fluoroscopic time was (18. 1 ± 3. 8) min, and contrast volume was (112. 0 ± 24. 5) ml, respectively. Post-procedurally, the residual stenosis of the main and the side branch were (10. 0 ± 2. 5)% and (10. 2 ± 5. 3)% , respectively. Conclusions The mono-ring culotte stenting is safe and feasible for treatment of true coronary bifurcation lesions, and may be superior to the conventional culotte stenting.
ABSTRACT
Objective:To explore influence of large dose atorvastatin therapy on ventricular remodeling in rats after myocardial infarction.Methods:A total of 48 male SD rats were randomly and equally divided into myocardial in-farction (MI)control group (received deionized water gavage),routine dose group (received atorvastatin 20mg/kg gavage four weeks before and after MI model was established)and large dose group (received atorvastatin 80mg/kg, in the same way as routine dose group).On two days after MI,hematoxylin eosin (HE)staining was used to detect pathological changes of myocardial tissue;four weeks later,Masson staining was used to evaluate myocardial fibrosis while echocardiography was used to assess cardiac chamber size and heart function,the results were compared among three groups.Results:Pathologic change:On two days after MI,color of stained myocardial tissue was uneven, muscular fiber arranged in turbulence,there were partial dissolved muscle focus and blockbuster collagenous fiber in MI control group;color of stained myocardial tissue was lack of even,arrangement of muscular fiber was irregular, there were less dissolved muscle focus and collagenous fiber in routine dose group;color of stained myocardial tissue was slightly uneven,muscular fiber still arranged in rules and there was very few dissolved muscle focus and still more survived myocardium in infarct area,and small amount of collagen fibrosis was found in myocardial cell clear-ance after four weeks in large dose group.Change of left ventricular structure and function:Compared with MI con-trol group and routine dose group,the left ventricular end-diastolic dimension [LVEDd,(7.68±0.54)ml vs.(7.06 ±0.68)ml vs.(6.37 ± 0.40)ml] significantly reduced in large dose group, left ventricular ejection fraction [LVEF,(33.0±7.9)% vs.(39.3±2.8)% vs. (48.7%±9.1)%]significantly rose (P <0.05,or <0.01);but those indexes no significant difference between MI control group and routine dose group (P =0.059,0.146 respec-tively).Conclusion:Large dose atorvastatin may significantly relieve ventricular remodeling after infarction.
ABSTRACT
Objective:To explore the influence of nephroblastoma over expressed gene (CCN3)on differentiation of bone marrow mesenchymal stem cells (BM-MSCs)into endothelial cells.Methods:BM-MSCs differentiation into endothelial cells induced by condition medium containing vascular endothelial growth factor (VEGF,50ng/ml)was regarded as positive control group.BM-MSCs differentiation into endothelial cells induced by condition medium con-taining recombined CCN3 protein (100 ng/ml)and VEGF (50 ng/ml)was regarded as CCN3 group,and BM-MSCs incubated in pure complete medium was regarded as negative control group.Immunofluorescence staining and semi-quantitative reverse transcription-polymerase chain reaction method (RT-PCR)were used to measure expression of von Willebrand factor (vWF)after 16d to evaluate endothelial cell differentiation,and semi-quantitative RT-PCR method was used to measured expression of Notch1 gene mRNA before and after induced BM-MSCs differentiation. Results:On 16d after induced BM-MSCs differentiation,some cells of positive vWF fluorescence staining were ob-served in positive control group,those of CCN3 group was significantly more than those of positive control group, and expression of vWF mRNA [ratio of optical density (OD): (0.550±0.090)]of CCN3group was significantly higher than that of positive control group (0.358±0.080),(P0.05 all),on 16d after induced differentiation, expressions of Notch1 gene mRNA in positive control group (0.232±0.047)and CCN3 group (0.352±0.029)were significantly higher than that of negative control group (0.132±0.033),P<0.01 all,it of CCN3 group was signifi-cantly higher that of positive control group (P<0.01).Conclusion:CCN3 can enhance BM-BMCs differentiation into endothelial cells by activating Notch1 signal pathway.
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the role of connexin 43-formed hemichannels in cell volume regulation induced by simulated ischemia/reperfusion (SI/R).</p><p><b>METHODS</b>Mouse cardiomyocytes isolated on a Langendorff apparatus with enzyme solution were aliquoted into control, SI/R and SI/R +octanol groups. Calcein-AM was used to stain the cells and the cell volume was measured with confocal microscope by stack scanning. Trypan blue was used to measure the cell viability after the treatments.</p><p><b>RESULTS</b>Calcein-AM staining and cofocal microscopy yielded stable and reproducible results for cell volume measurement. Mouse cardiomyocytes subjected to simulated SI/R showed obvious cell swelling as compared with the control cells [(126∓6)% vs 100%, P<0.05], and octanol preconditioning significantly attenuated the cell swelling [(113∓6)%, P<0.05]. SI/R caused a significant reduction of the cell viability compared to the control cells [(19∓2)% vs (45∓3)%, P<0.01], and octanol preconditioning obviously reduced the viability of the cells with SI/R challenge [(31∓2)%, P<0.01].</p><p><b>CONCLUSION</b>Connexin 43-formed hemichannels are involved in the regulation of cardiomyocyte volumes induced by SI/R challenge, and octanol can alleviate the cell swelling to enhance the viability of the cardiomyocytes following SI/R.</p>
Subject(s)
Animals , Mice , Cell Line , Cell Size , Cell Survival , Connexin 43 , Metabolism , Ischemic Preconditioning, Myocardial , Methods , Mice, Inbred C57BL , Myocardial Reperfusion Injury , Metabolism , Pathology , Myocytes, Cardiac , Pathology , Octanols , PharmacologyABSTRACT
Objective To compare the procedural difficulty index (PDI) and immediate outcome (IM) of percutaneous coronary intervention (PCI) in patients with various stages of myocardial infarction.Methods Ninety-four patients with myocardial infarction were divided into three groups, direct PCI(n=38), delayed PCI(n=22) and late PCI(n=34). The characteristics of infarct-related coronary artery, PDI and IM of PCI were evaluated angiographically, and severe procedural complications (SPC) and major adverse cardiac events (MACE) during hospitalstay were documented. Results In the three groups, PDI was 1.47 ?1.79, 1.82 ?1.72 and 2.85 ?2.83, respectively (P