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AIM: To investigate the effect of autologous bone marrow mononuclear cells (BM-MNCs) local transplantation (BM-LT) on a rabbit model of hindlimb ischemia. METHODS: After unilateral femoral artery and its branches were surgically ligated and excised in rabbits (n=14) 1 hour or so, autologous BM-MNCs were injected into the ischemic skeletal muscles in 7 rabbits, and phosphate buffered saline for another 7 ones as control. Hemodynamics parameters were measured at day 0, day 7 and day 28, respectively. On day 28, the animals were sacrificed in groups for pathological analysis. RESULTS: Compared with control group, color Doppler flow image in BM-LT group was recovered significantly, and its' systolic peak velocity and end-diastolic velocity were also improved (P
ABSTRACT
Percutaneous coronary intervention(PCI) can effectively restore reperfusion of ischemic myocardium in patients with coronary artery disease.But currently PCI treatment is just available for a small proportion of the patients with coronary artery disease,so there was an insufficiency of PCI in patients with coronary artery disease.From the perspective of ethics,the reasons for the phenomenon and some feasible strategies for the problem are also analyzed in this paper.
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Objective To explore the expansion and differentiation of bone marrow endothelial progenitor cells in vitro. Methods Rabbit bone marrow mononuclear cells were isolated by gradient density centrifugation.Attached cells were cultured and in endothelial cell growth supplement(ECGS) from bovine pituitary.Cell multiplication was observed,and their biological characteristics were detected. Results The number of attached cells increased 5～8 fold with each expanded.Cobblestone like structure was observed when the cells lined in single layer.Expanded cells expressed von Willebrand factor(vWF) and could ingest Dil acLDL. ConclusionEndothelial progenitor cells,which exist within adult bone marrow,have the potential to be expanded and differentiated when stimulated by ECGS in vitro.