ABSTRACT
Introduction: It is a known fact that human sperms, producereactive oxygen species in small amounts, which is usefulfor normal functioning of the sperm mainly sperm oocyteinteraction acrosome reaction and capacitation. Recentresearch has shown that this ROS in increased amounts is acause of male infertility.Material and methods: It is a prospective control trial todetermine the abnormal pattern of reactive oxygen speciesin semen sample of patients with male factor infertility andto determine the effects of antioxidants in semen sampleof patients with high ROS in semen sample. 150 patientswere selected who were undergoing IVF treatment. Patientswere divided into three groups according to the severity ofabnormality in the semen analysis. The ROS levels of all thepatients were measured and compared with 50 normal fertiledonors. The patients whose ROS levels was found to be highwere then given antioxidants for 2 months and their ROSlevels in semen measured for improvement.Results: The ROS levels were significantly high in all thethree groups compared to fertile donors. The patients withhigh R0S levels were then treated with antioxidants and it wasseen that all the three groups had significant improvement intheir ROS levels.Conclusion: So, to conclude it is hence proved by my studythat ROS level measurement is useful for patients with malefactor infertility and the use of antioxidants significantly helpsin reducing ROS levels in semen.