ABSTRACT
The aim of the present investigation is to study the fungal flora of young and matureleaves of S. rebaudiana. Young and mature leaves of S. rebaudiana were used for isolation offungi. Phylloplane mycoflora were isolated from leaves of S. rebaudiana by using threemethods i.e. dilution plate method, leaf impression method and leaf washing method. Dilutionplate method and leaf washing method were applied for quantitative analysis and leafimpression method for qualitative analysis. A total of 35 fungal species comprisingZygomycetes, Ascomycetes and Deuteromycetes including sterile forms were isolated duringthe course of investigation. A total of thirteen fungal species viz. Aspergillus niger, A. flavus, A.fumigatus, Penicillium cyclopium, P. chrysogenum, Cladosporium elatum, C. cladosporioides,Mucor etc. were isolated from mature leaves. Eleven fungal species were found by leafimpression method, eight by leaf washing method and six by dilution plate method.Aspergillus, Penicillium and Cladosporium species were found to be dominant among all thespecies. Phylloplane fungal population on mature leaves of S. rebaudiana was 1.68×102 /cm2leaf area. Thus, the outcome of study presents a clear picture about fungal population anddiversity in phylloplane of S. rebaudiana mature leaves.
ABSTRACT
In present study, Althaea officinalis seed extracts and essential oil were screened for antimicrobial activity against five bacteria and one fungi responsible for dominant, lethal or opportunistic infection of respiratory regions. The maximum inhibition was noted by essential oil against Streptococcus pyogenes (21.3±0.28 mm) and Haemophilus influenzae (19.0±0.50 mm) at 200 mg/ml. The minimum inhibitory concentration values for methanol extract was 3.12-12.5 mg/ml. The antifungal activity noted highest with 41.28% by essential oil and 36.27% inhibition by aqueous extract represented by dosage-response curve.