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ABSTRACT: The mysterious ancient Mesoamerican Indian crop chia (Salvia hispanica) is revived and expanding worldwide due to its richness of valuable nutraceuticals such as α-linolenic acid (ALA), antioxidants, food fiber, gels, and proteins. We carried out a pilot experiment on chia planting in non-frost Sichuan Basin, at Hechuan Base (30˚0′ 43″ N, 106˚7′ 41″ E, 216 m), Southwest University, Chongqing, China. The split-plot trial contained two factors, 3 spring-summer sowing times as main plots, and 6 densities as subplots, with 3 replicates. Phenological, botanical, adversity, yield, and seed quality traits were investigated. Plants were very tall, suffered from lodging, and flowered in mid-October. Sichuan Basin can be considered as a north edge for growing chia, with low yield (680 kg/hectare) because of insufficient seed filling and maturation in autumn-winter season (1000-seed weight of 1.14 g). However, its ALA content is 5 percent points higher than the seed-donor commercial bottle (65.06%/63.96% VS 59.35%/59.74% for black/white seeds), accompanied by decrease oleic and stearic acid, while linoleic acid and palmitic acid are equivalent. Considering its short-day habit, it is recommended to try sowing in middle summer (from late June to early August) to avoid too long growing period, excessive vegetative growth, and waste of field and climate resources caused by spring-summer sowing. Furthermore, winter sowing of chia with mulch cover could also be tried, with an expectation of harvesting in summer. Most importantly, only when the photoperiod-insensitive early flowering stocks are created, chia can be recommended as a low-risk crop to the farmers of this region.
RESUMO: A chia (Salvia hispanica) é cultivada em todo o mundo por sua riqueza de nutrientes nutracêuticos valiosos, tais como a-ácido linolênico (ALA), antioxidantes, fibras alimentares, géis e proteínas. Entretanto, não há informações sobre sua performance agronômica se cultivada aos 30˚N na China. Assim, realizou-se um experimento com o cultivo de chia na base Hechuan (30°0'43"N, 106°7'41"E, 216m, que não apresenta geada) da Southwest University, Chongqing, China. O delineamento em parcela subdividida contém dois fatores,três épocas de semeadura na primavera-verão como parcelas principais e seis densidades de sementes como subparcelas, com três repetições. Foram investigados os caracteres fenológicos, botânicos, de adversidade, rendimento e qualidade da semente. As plantas se tornaram altas, acamarame floresceram em meados de outubro. A bacia de Sichuan pode ser considerada como uma fronteira limítrofe norte para o crescimento da chia, com baixo rendimento (680kg ha-1) devido ao enchimento e amadurecimento insuficientes na estação outono-inverno (peso de 1000 sementes de 1,14g). No entanto, o seu conteúdo de ALA é de 5 pontos percentuais mais elevado do que a semente comercial, 65,06%/63,96% contra 59,35%/59,74% para as sementes pretas/brancas, respectivamente, acompanhado por diminuição de ácido oleico e ácido esteárico, enquanto que o ácido linoleico e o ácido palmítico são equivalentes. Considerando o seu hábito de dia curto, recomenda-se semear no meio do verão,de junho a início de agosto, para evitar um tempo de cultivo muito longo, desenvolvimento vegetativo excessivo e desperdício de recursos de campo e clima causados pela semeadura de primavera-verão. Além disso, a semeadura de inverno da chia com cobertura morta também poderia ser realizada, com expectativa de colheita no verão. Mais importante ainda, somente quando os estoques de floração precoce insensíveis ao fotoperíodo são criados, pode-se recomendar como uma cultura de baixo risco para os agricultores desta região.
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The mediastinal lymph node tuberculous abscesses (MLNTAs) are secondary to mediastinal tuberculous lymphadenitis.Surgical excision is often required when cold abscesses form.This study was aimed to examine video-assisted thoracoscopic surgery (VATS) for the treatment of MLNTA.Clinical data of 16 MLNTA patients who were treated in our hospital between December 1,2013 and December 1,2015 were retrospectively analyzed.All of the patients underwent the radical debridement and drainage of abscesses,and intrathoracic lesions were removed by VATS.They were also administered the intensified anti-tuberculosis treatment (ATT),and engaged in normal physical activity and follow-up for 3 to 6 months.The results showed that VATS was successfully attempted in all of the 16 MLNTA patients and they all had good recovery.Two patients developed complications after surgery,with one patient developing recurrent laryngeal nerve injury,and the other reporting poor wound healing.It was concluded that VATS is easy to perform,and safe,and has high rates of success and relatively few side-effects when used to treat MLNTA.
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@#Objective To explore the diagnostic and treatment value of computed tomography (CT)-guided embolization coil localization of pulmonary nodules accurately resected under the thoracoscope. Methods Between October 2015 and October 2016, 40 patients with undiagnosed nodules of 15 mm or less were randomly divided into a no localization group (n=20, 11 males and 9 females with an average age of 60.50±8.27 years) or preoperative coil localization group (n=20, 12 males and 8 females with an average age of 61.35±8.47 years). Coils were placed with the distal end deep to the nodule and the superficial end coiled on the visceral pleural surface with subsequent visualization by video-assisted thoracoscopic (VATS). Nodules were removed by VATS wedge excision using endo staplers. The tissue was sent for rapid pathological examination, and the pulmonary nodules with definitive pathology found at the first time could be defined as the exact excision. Results The age, sex, forced expiratory volume in the first second of expiration, nodule size/depth were similar between two groups. The coil group had a higher rate of accurate resection (100.00% vs. 70.00%, P=0.008), less operation time to nodule excision (35.65±3.38 min vs. 44.38±11.53 min, P=0.003), and reduced stapler firings (3.25±0.85 vs. 4.44±1.26, P=0.002) with no difference in total costs. Conclusion Preoperative CT-guided coil localization increases the rate of accurate resection.
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<p><b>OBJECTIVE</b>To generate phage-displayed anti-idiotypic antibody single chain variable fragments (anti-Id ScFv) to MG7 monoclonal antibody (McAb) directed against gastric carcinoma so as to lay a foundation for developing anti-Id ScFv vaccine of the cancer.</p><p><b>METHODS</b>Balb/c mice were immunized i.p. with MG7 McAb conjugated with keyhole limpet hemocyanin (KLH), and mRNA was isolated from the spleens of the immunized mice. Heavy and light chain (VH and VL) genes of antibody were amplified separately and assembled into ScFv genes with a linker DNA by PCR. The ScFv genes were ligated into the phagemid vector pCANTAB5E and the ligated sample was transformed into competent E. coli TG1. The transformants were infected with M13K07 helper phage to yield recombinant phages displaying ScFv on the tips of M13 phage. After 4 rounds of panning with MG7, the MG7-positive clones were selected by ELISA from the enriched phages. The types of the anti-Id ScFv displayed on the selected phage clones were preliminarily identified by competition ELISA.</p><p><b>RESULTS</b>The VH, VL and ScFv DNAs were about 340 bp, 320 bp and 750 bp respectively. Twenty-four MG7-positive clones were selected from 60 enriched phage clones, among which 5 displayed beta or gamma type anti-Id ScFv.</p><p><b>CONCLUSION</b>The anti-Id ScFv to MG7 McAb can be successfully selected by recombinant phage antibody technique, which paves a way for the study of prevention and cure of gastric carcinoma by using anti-Id ScFv.</p>