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Objective To investigate the effect of inhibiting lipid synthesis on human skeletal rhabdomyosarcoma cells and its molecular mechanism.Methods The mRNA expression levels of lipid synthesis related genes sterol regulatory element binding protein 1(SREBP1)and squalene cyclooxygenase(SQLE)in human skeletal muscle cells(HSMC)and human skeletal rhabdomyosarcoma cells were detected by tumor gene expression database and verified by qRT-PCR.The concentration of methyl-β-cyclodextrin(mβCD)was determined by cell proliferation as-say.The control group consisted of three human skeletal rhabdomyosarcoma cell lines(RD,SJCRH30,A673),while the experimental group comprised three human skeletal rhabdomyosarcoma cell lines treated with 1mmol/L mβCD.Plate clone formation assay,soft agar,colony formation assay,cell migration assay and cell invasion assay,and tumor formation in nude mice employed to assess changes in proliferation,migration,invasion,and tumor growth of human skeletal rhabdomyosarcoma cells between the control group and mβCD treatment group.The mo-lecular mechanism of mβCD inhibiting the malignancy of human skeletal rhabdomyosarcoma cells was explored by lipoomics and triglyceride(TG)detection.Results Compared with HSMC,the expressions of lipid synthesis relat-ed genes SREBP1 and SQLE significantly increased in human skeleton rhabdomyosarcoma cells(P＜0.001).Compared with the control group,the proliferation,plate cloning,migration,invasion and colony formation ability of human skeleton rhabdomyosarcoma cells in the mβCD group significantly decreased(P＜0.05).The growth of tumor volume and weight in nude mice was also significantly reduced(P＜0.05).The lipidomics results and TG kit analysis revealed a significant reduction in TG content in the mβCD group compared to the control group(P＜0.01).Conclusion m βCD may inhibit the malignant biological behaviors such as proliferation,migration and in-vasion of human skeleton rhabdomyosarcoma cells by reducing TG and other lipid metabolism.
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Objective @#To investigate the effects of intra-articular injection of alendronate on the mandibular condyle in ovariectomized rats.@*Methods @#Sixty female rats were randomly divided into five groups: ovariectomy with vehicle treatment alone, early alendronate treatment at ovariectomy, late alendronate treatment at 4 weeks after ovariectomy, shamoperation with vehicle treatment, and the normal control rats. The changes in subchondral bone were evaluated by micro-computed tomography (Micro-CT), tartrate-resistant acid phosphatase (TRAP) staining and real-time quantitative polymerase chain reaction (RT-PCR). @*Results @# Compared with late alendronate treatment, early alendronate treatment improved microstructural properties of the subchondral bone, with higher bone volume ratio (46.4 + 2.5 vs 37.5 + 2.1; P= 0.038), increased trabecular thickness (47.3 + 1.7 vs 34.6 + 1.4; P = 0.029), elevated trabecular number (8.5 + 0.6 vs 6.2 + 0.3; P = 0.041) and lower trabecular separation (30.2 + 1.6 vs 37.7 + 2.6; P = 0.034). Fewer TRAP-positive cells (4.2 + 0.2 vs 6.8 + 0.4; P = 0.019) and a higher OPG/RANKL ratio (0.38 + 0.01 vs 0.25 + 0.03; P = 0.043) in the subchondral bone were observed in the animals with early treatment group compared to late treatment or ovariectomy/vehicle treatment group. @*Conclusion @#Our results suggest the therapeutic potential of intra-articular alendronate injection in the treatment of osteoporosis-associated temporomandibular disorders.
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Objective To understand the current management situation of hand hygiene (HH)among health care workers(HCWs)in stomatology medical institutions in Zunyi,and provide basis for the scientific management of HH.Methods In October-December 2014,the on-site observation and questionnaire survey were adopted to investigate current management situation of HH in 127 stomatology medical institutions in Zunyi.Results The rates of qualified installation of sink,faucet,detergent,alcohol-based hand rub,and hand-drying facilities were 60.63%, 11 .02%,92.13%,22.05%,and 37.80% respectively.Except detergent and sink,the installation of other HH supplies in stomatology medical institutions in Zunyi were not ideal.The correct rate of HH among HCWs was 33.07%(42/127),qualified rates of HH methods among HCWs in departments of stomatology,comprehensive out-patient departments, stomatological clinics,and teeth-cleaning clinics were 50.00%,23.08%,23.53%,and 0 respectively.Conclusion HH facilities are not perfect in primary stomatology medical institutions in Zunyi,health ad-ministrative departments’supervision on HCWs’HH is inadequate,the awareness rate of HH knowledge and com-pliance to HH among HCWs are both low.
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<p><b>AIM</b>To investigate the protective effect of exogenous carbon monoxide (CO) on the liver injury induced by ischemia/reperfusion (I/R) of hind limbs in rats.</p><p><b>METHODS</b>100 SD rats were divided randomly into sham operated group (S), S+ CO group (SC), I/R group (I/R), I/ R+ CO group (RC). A rat model of ischemia in hind limbs and the reperfusion liver injury was established with the occlusion of the femoral arteries for 4 h and re-opening for 6 - 72 h, 10 d. The rats in SC and RC groups were exposed to air containing CO (the volume traction of CO: 0.05%) for 2 h before and after reperfusion or the corresponding control time point, while the other two groups were exposed to the routine air. The pathologic changes of liver tissue were morphologically observed by HE stain. Serum GPT activity was tested by Automatic Biochemical Analyzer. The percentage of apoptosis, expression levels of bax and bcl-2 protein in liver tissue were detected by Flow Cytometry.</p><p><b>RESULTS</b>There was no difference between S and SC groups. Compared with SC group: (1) Pathological changes in liver tissue were significant in I/R and RC groups. (2) The serum GPT activity of I/R and RC groups was obviously increased. (3) In IR and RC groups, the percentage of apoptosis in liver tissue was all significantly increased. (4) The bax expression level was significantly increased. Compared RC group with I/R group: (1) Pathological change was slight. (2) The serum GPT activity was depressed. (3) The percentage of apoptosis and expression level of bax protein in liver tissue were depressed. (4) The expression level of bcl-2 protein in liver tissue was increased.</p><p><b>CONCLUSION</b>Exogenous CO could attenuate liver tissue injury induced by limbs I/R in rats.</p>