ABSTRACT
AIM To prepare the liposomes loaded with celastrol,tanshinone Ⅱ A and tanshinone Ⅱ A sodium sulfonate and to evaluate the effects of preparation technologies and entrapment materials on the encapsulation efficiencies.METHODS The liposomes for three constituents were prepared by film dispersion method and reverse phase evaporation method,respectively.50％ Lecithin high potency,80％ egg yolk lecithin,92％ lecithin high potency and complex phospholipid (5 ∶ 1 for ratio of dipalmitoyl phosphatidylcholine to 50％ lecithin high potency) were served as entrapment materials.The encapsulation efficiencies were thus calculated based on the measured ean sizes and polydispersity of the liposomes.RESULTS Celastrol and tanshinone Ⅱ A sodium sulfonate liposomes accomplished by reverse phase evaporation method got the highest encapsulation efficiency when entrapped with 80％ egg yolk lecithin and 92％ lecithin high potency,respectively,which were higher than those prepared by film dispersion method.And celastrol liposomes exhibited an even superior performance than tanshinone Ⅱ A sodium sulfonate liposomes.Tanshinone Ⅱ A lipisome prepared by film dispersion method and then entrapped with 50％ lecithin high potency obtained its the highest value of encapsulation efficiency,which was much lower than those of another two liposomes.CONCLUSION Reverse phase evaporation method is appropriate for hydrophobic celastrol with multiple hydrogen bonds and hydrophilic sodium tanshinone Ⅱ A without hydrogen bonds to prepare their liposomes,while film dispersion method is applicable to hydrophobic tanshinone Ⅱ A without hydrogen bonds.
ABSTRACT
The pellet-preparating theories and processes of two kinds of methods of extrusion-spheronization and fluid-bed coating method were compared. The micromeritic properties, yield and in vitro dissolution of Danggui Buxue pellets prepared by the two methods were determined. Results showed that the Danggui Buxue pellets prepared by extrusion-spheronization were all spheral with smooth surface, the yield of those pellets was higher and in vitro dissolution of those pellets was more satisfactory. So the two kinds of methods were all suitable to prepare Dang-gui-bu-xue pellets, but Extrusion-spheronization was superior to fluid-bed coating method.
Subject(s)
Chemistry, Pharmaceutical , Methods , Dosage Forms , Drug Compounding , Methods , Drugs, Chinese Herbal , ChemistryABSTRACT
<p><b>OBJECTIVE</b>To study the pharmacokinetics of pueraria flavonoids(PF) from Pueraria lobata.</p><p><b>METHOD</b>An anti-oxidant pharmacodynamics effect method was quoted to detect the antioxidant ability of PF contained in rats plasm after oral delivered, measures the fluorescence intensity, and calculates the concentration of components in plasma, then we can get the plot of the change of the content of PF contained in rats plasm, and then we can get the pharmacokinetics of Pueraria Flavonoids pellets.</p><p><b>RESULT</b>The AUC of Pueraria lobata isoflavone pellets is 4.40-fold of Yufengningxin tablets.</p><p><b>CONCLUSION</b>It can enhance the bioavailability of PF by made it to pellets.</p>