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Rev. bras. pesqui. méd. biol ; Braz. j. med. biol. res;47(12): 1050-1056, 12/2014. graf
Article in English | LILACS | ID: lil-727667

ABSTRACT

People who suffer from traumatic brain injury (TBI) often experience cognitive deficits in spatial reference and working memory. The possible roles of cyclooxygenase-1 (COX-1) in learning and memory impairment in mice with TBI are far from well known. Adult mice subjected to TBI were treated with the COX-1 selective inhibitor SC560. Performance in the open field and on the beam walk was then used to assess motor and behavioral function 1, 3, 7, 14, and 21 days following injury. Acquisition of spatial learning and memory retention was assessed using the Morris water maze on day 15 post-TBI. The expressions of COX-1, prostaglandin E2 (PGE2), interleukin (IL)-6, brain-derived neurotrophic factor (BDNF), platelet-derived growth factor BB (PDGF-BB), synapsin-I, and synaptophysin were detected in TBI mice. Administration of SC560 improved performance of beam walk tasks as well as spatial learning and memory after TBI. SC560 also reduced expressions of inflammatory markers IL-6 and PGE2, and reversed the expressions of COX-1, BDNF, PDGF-BB, synapsin-I, and synaptophysin in TBI mice. The present findings demonstrated that COX-1 might play an important role in cognitive deficits after TBI and that selective COX-1 inhibition should be further investigated as a potential therapeutic approach for TBI.


Subject(s)
Animals , Brain Injuries/complications , Cerebral Cortex/injuries , Cyclooxygenase 1/physiology , Cyclooxygenase Inhibitors/therapeutic use , Learning/drug effects , Memory Disorders/drug therapy , Pyrazoles/therapeutic use , Blotting, Western , Brain-Derived Neurotrophic Factor/metabolism , Cerebral Decortication , Cyclooxygenase 1/metabolism , Disease Models, Animal , Dinoprostone/analysis , Dinoprostone/metabolism , Enzyme-Linked Immunosorbent Assay , Hippocampus/metabolism , /blood , Maze Learning/drug effects , Memory Disorders/etiology , Memory Disorders/metabolism , Proto-Oncogene Proteins c-sis/metabolism , Recovery of Function/drug effects , Synaptophysin/analysis , Synaptophysin/metabolism
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