ABSTRACT
This review made a brief summary of several pharmacokinetic methods of traditional Chinese medicine (TCM ) prescription including the methods of biological effects ,plasma concentration ,integrated Pharmacokinetics of multiple effective components of TCM prescription and integrated pharmacokinetic‐pharmacodynamic of TCM prescription .The corresponding characteristics ,advantages and disadvantages of each of these methods were analyzed .And the progresses of the pharmacokinetic methods of TCM prescription were concluded and prospected .
ABSTRACT
Objective To build hypoxia/reoxygenation injury model in cultured neonatal rat cardiomyocyte and screen active components from Shexiang Baoxin Pill ( SBP) absorbed in blood against hypoxia/reoxygenation injury .Methods Cardiomyocytes were isolated and purified from hearts of neonatal Sprague Dawley rats (1~3 days old) and were used to build hypoxia/reoxygenation injury model.The components of SBP absorbed in blood were screened by methyl thiazolil tetracolium (MTT) colorimetic method.Results SBP showed significant protective effect against cardiomyocytes hypoxia /reoxygenation injury atthe concentration of 50 μg/ml.Ginsen-oside Rb1, Rb2, bufalin and muscone of twenty components from SBP absorbed in blood also possessed significant protective effect a -gainst cardiomyocytes hypoxia/reoxygenation injury .Conclusion SBP have the protective activity against cardiomyocytes hypoxia /reoxygenation injury , and ginsenoside Rb1, Rb2, bufalin, muscone are the main active components of SBP .This experiment offered basis for further pharmacodynamics and mechanism study of SBP .