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Proteomics was first proposed by Marc Wikins, et al in 1995. It refers to the investigation of all the proteins expressed in a set of genomes. With the development of mass spectrometry technology, it more and more extensively apply to the field of radiation. In particular, due to the widespread application of nuclear and radiation technologies has greatly increased, the probability of nuclear and radiation accidents, and the exposure of large-scale populations are increased. Therefore, simple, rapid, and high-throughput measurement of acute radiation exposure is necessary. The application of proteomics technology to study the molecular biological effects of radiation and the discovery of radiation biomarkers provide the possibility for rapid high-through put dose assessment. We reviewed the recent development of proteomics and its application in the field of radiation.
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Objective To investigate the risk factors of malignancy from gallbladder polyps (GBPs).Methods The retrospective case-control study was conducted.The clinicopathological data of 604 patients with GBPs who were admitted to the Chinese PLA General Hospital between January 2011 and October 2016 were collected.There were 255 males and 349 females,aged from 19 to 88 years,with an average age of 47 years.There were 565 of 604 patients with benign GBPs and 39 with malignant GBPs.Observation indicators:(1) risk factors analysis of malignancy from GBPs;(2) the predictive ability of polyp diameter for malignancy from GBPs.Measurement data with normal distribution were expressed as Mean ± SD,measurement data with skewed distribution were described as M (range),and the univariate analysis was done using the t test or rank-sum test.Count data were described by the absolute amount,and the univariate analysis was done using the chi-square test or Fisher exact probability.The indicators with P＜0.05 in the univariate analysis based on clinical application were used in the Logistic regression models for multivariate analysis.The receiver operating characteristic (ROC) curve was drawn.The Youden index was calculated to analyze the predictive ability of polyp diameter for malignancy from GBPs.Results (1) Risk factors analysis of malignancy from GBPs:results of univariate analysis showed that age,polyp diameter,polyp number,chronic cholecystitis and carcinoembryonic antigen (CEA) level were related factors affecting malignancy from GBPs (t=-5.50,Z=-9.65,x2=15.92,312.65,Z=-1.78,P＜0.05).The results of multivariate analysis showed that age,polyp diameter and polyp number were independent factors affecting malignancy from GBPs (odds ratio =1.088,45.190,9.655,95％ confidence interval:0.974-1.159,4.312-121.139,0.890-117.551,P＜0.05).(2) The predictive ability of polyp diameter for malignancy from GBPs.The sensitivity and specificity predicting malignancy from GBPs were 94.9％ and 81.2％ in patients with polyp diameter =10 mm and Youden index =0.761,89.7％ and 90.6％ in patients with polyp diameter =12 mm and Youden index =0.803,84.6％ and 92.6％ in patients with polyp diameter =13 mm and Youden index =0.772,respectively.Conclusions The age,polyp diameter and polyp number are the independant factors affecting malignancy from GBPs.The malignancy possibility from GBPs is higher in patients with the age ＞ 50 years,polyp diameter ＞ 12 mm,solitary polyp,and should undergo surgical therapy actively.
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<p><b>OBJECTIVE</b>To set up the fingerprint of HPCE for medicinal material Eupolyphaga Steleophaga.</p><p><b>METHOD</b>Separation was performed at 25 degrees C on an Agilent uncoated silica capillary column (40 cm x 75 microm) with 20 mmol x L(-1) borax buffer solution (pH 9.44) as CE buffer. The isolating voltage was 13 KV, and the DAD detection was set at 265 nm.</p><p><b>RESULT</b>The characteristic peak of fingerprint of Eupolyphaga Steleophaga was consisted of 6 common peaks.</p><p><b>CONCLUSION</b>The method can be used for the quality control of Eupolyphaga Steleophaga.</p>
Subject(s)
Animals , Female , Electrophoresis, Capillary , Methods , Insecta , Chemistry , Materia Medica , Medicine, Chinese Traditional , Methods , Quality Control , Reference Standards , UracilABSTRACT
Objective To study the expression of elastic fibers,Collagen type Ⅳ and CD44v6 in the metastasis of the pulmonary adenocarcinoma.Methods In paraffin-embedded specimens from 56 cases of pulmonary adenocarcinoma,the elastic fibers were stained with histochemical method and the expressions of collagen type Ⅳ and CD44v6 were detected with immunohistochemical method.Results The positive expression rates of elastic fibers,collagen type Ⅳ and CD44v6 were 11.5%(3/26),11.5%(3/26) and 80.8%(21/26)in the group with lymph node metastasis,and were 69.0%(20/29),55.2%(16/29) and 24.1%(7/29) in the group without lymph node metastasis,respectively.The expression of elastic fibers,collagen type Ⅳ and CD44v6 were significant difference in the two groups with and without lymph node metastasis(P=0.000).There was statistically significant correlation among elastic fibers,collagen type Ⅳ,CD44v6 and lymph node metastasis(all P