ABSTRACT
OBJECTIVE@#To explore the eff ect of pulchinenoside (PULC) on fi broblast-like synoviocytes (FLS) apoptosis in adjuvant arthritis (AA) rats.@*METHODS@#A total of 60 SD rats were randomly divided into 8 groups: A normal control group, an AA group, a low PULC group (50 mg/kg), a middle PULC group (100 mg/kg) or a high PULC group (150 mg/kg) and an ibuprofen (8 mg/kg) group (n=10 per group). FLS from the AA rats was cultured. The expression of Bcl-2, Bax, caspase-3 and the FLS proliferation were detected by the real time qPCR and MTT, respectively. The expression of IL-6 and IL-8 in culture medium was detected by ELISA.@*RESULTS@#Compared with the AA group, the Bcl-2 expression was down-regulated (all P<0.05), the Bax and caspase-3 expression was up-regulated (all P<0.05), and the FLS proliferation was inhibited (all P<0.05). The IL-6 and IL-8 expression was suppressed in the FLS in the PULC groups at different dosages (all P<0.05) as well as in the ibuprofen group (P<0.05).@*CONCLUSION@#PULC may inhibit the FLS proliferation in AA rats by increase in FLS apoptosis.
Subject(s)
Animals , Rats , Apoptosis , Arthritis, Experimental , Caspase 3 , Metabolism , Fibroblasts , Cell Biology , Interleukin-6 , Metabolism , Interleukin-8 , Metabolism , Proto-Oncogene Proteins c-bcl-2 , Metabolism , Pulsatilla , Chemistry , Rats, Sprague-Dawley , Synovial Membrane , Cell Biology , bcl-2-Associated X Protein , MetabolismABSTRACT
0.05). In comparison to control group (26.5%?14.3%), the fatigue index (FI) decreased remarkably in CMV+PEEP group (12.2%?6.4%, P