ABSTRACT
Objective To observe the effects of hyperbaric oxygen (HBO) on learning and memory ability and on the level of myelin basic protein (MBP) in the brain tissue of rats with delayed neurological sequelae (DNS) after acute carbon monoxide poisoning (COP).Methods Forty-eight male SD rats had their cognitive performance assessed with the Morris water maze.After basic training with the Morris water maze and screening,the rats were randomly divided intoanormalcontrol (NC) group (n=11),a COP group (n=17) and an HBO group (n=17).Pure CO gas was injected intraperitoneally to establish acute COP in the latter two groups.The NC group received a similar injection of air.The HBO group was given HBO therapy.The rats in each group were tested for changes in their learning and memory abilities using the Morris water maze.On the 21 st day after the treatment,paraffin tissue sections of the rats' brains were subjected to immunohistochemical (IHC) examination and Western blotting (WB) was used to detect any expression of MBP in the brain tissue.Results After 21 days,morbidity among the COP group was 64.3％,while it was 26.7％ in the HBO group,a significant difference.The average maze escape latency in the COP group was significantly longer than in the HBO group.IHC staining and Western blotting showed that MBP in the hippocampal tissue of the COP group was significantly lower than that in the HBO group.In gray scale comparisons of the rats' brain tissue,that from the NC group was significantly better than that from the COP and HBO groups,but that from the HBO group was significantly better than that from the COP group.Conclusion HBO can effectively reduce DNS after acute COP,mitigate the severity of DNS,reduce demyelination of brain tissue and thus play an important role in protecting brain cells.
ABSTRACT
Objective To explore the influence of hyperbaric oxygen(HBO)on the life-spans of tumor-bearing mice. Methods Twenty-eight male Balb/c mice were randomly and evenly divided into:a celiac-tumorgroup(inoculated with tumor cells in the abdominal cavity);a celiac-tumor-HBO group(inoculated and then ex-posed to hyperbaric oxygen);a back-tumor group(inoculated under the skin of the back);and a back-tumor-HBOgroup(inoeulatedunder the skin of the back and then exposed to hyperbaric oxygen).S-180 carcinoma cells were in-oeulated,and then HBO was administered once a day.The life-spans and any skin ulceration were observed. Re-sults The average life-spans were(27.6±4.5)days in the celiac.tumor group,(24.0±2.9)days in the celiac-tumor-HBO group,(63.0±21.8)days in the back.tumor group and(35.0±8.9)days in the back-tumor-HBOgroup.The difference in average life-span between the celiac-tumor group and the celiac-tumor-HBO gmup was not significant.The difference in life-span between the back-tumor group and the back.tumor.HBO group was significant.Ulcers occurred in 4 mice in the back-tumor group and 4 in the back-tumor-HBO group.The times of ulcer formation were significantly different between the back-tumor group and the back-tumor-HBO group. Conclusions The life-spans of mice in the back-tumor-HBO group were shortened by HBO exposure,but life-spans in celiac-tumor-HBOgroup were not.Ulcer formation was postponed by HBO in the back-tumor-HBO group.