ABSTRACT
Objective To analyze and compare fine particle fraction(FPF)and particle size distribution(PSD)measured by twin-stage impactor(TSI),Andersen cascade impactor(ACI)and next generation pharmaceutical impactor(NGI). Methods Self-made metered dose inhaler(MDI) of fluticasone propionate was selected as the model drug,and the FPF and PSD were determined by TSI,ACI and NGI. Results The device of TSI was simple,and fine particle drug dose with less than 6.4 micrometer aerodynamic di?ameter size was acquired quickly,and the recovery rate was relatively high under the good seal,but aerodynamic diameter distribution was not obtained. In contrast,both the aerodynamic diameter and particle size distribution were obtained with ACI and NGI. Different from ACI,for NGI it was not necessary to wash the sieves,although NGI is heavy. So,the operation of NGI is simpler. Conclusion NGI can be chosen as the priority for the determination of the aerodynamics related parameters.
ABSTRACT
Objective:To establish the HPLC fingerprint of Prunella Species from different places and evaluate it by cluster analysis.Methods:The analysis method of HPLC ngerprint was established.Alltima C18(4.6mm?250mm,5?m) was utilized for separation.The mobile phase consisted of methanol(A) and 0.5% glacial acetic acid(B) with a ow-rate of 1.0ml/min.The detection wavelength was 280nm and the injection volume was 20?l.The column temperature was 30℃.The HPLC ngerprints of Prunella Species from three di erent places were determined and analyzed by SPSS.Results:There is a big di erence among ngerprints of Prunella from di erent places.Prunella from Hubei,Anhui,Jiangxi,Guizhou,Guangxi,Sichuan were divided into a class;Henan,Jiangsu,Zhejiang were divided into another class.Conclusion:the eatsblished ngerprint showed characteristics of Prunella Species from di erent places obviously,and the attribute of Prunella Species can be distinguished e ectively by cluster analysis method.