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OBJECTIVE@#To systematically evaluate the clinical effect of cultured milk products as adjunctive therapy in the anti- Helicobacter pylori (H. pylori) treatment. @*METHODS@#The randomized controlled trials (RCT) and Quasi-randomized controlled clinical trials (Quasi-RCT), which were used to evaluate the efficacy and safety of eradicating H. pylori by fermented milk-based routine treatment, were searched and collected in Pubmed, Embase, CNKI (China National Knowledge Infrastructure), CBM (Chinese BioMedical Literature Database), Wangfang Database, VIP (VIP Citation Database) from establishment of these database to February 2015. The combined relative risk (RR) of H. pylori eradication rate and the rate of side effects were analyzed. Sub-group and sensitivity analysis was performed, and the publication bias was also tested. @*RESULTS@#A total of 9 studies including 1 644 cases were identified. The H. pylori eradication rate was 79.5% in fermented milk products combined with routine therapy, and 67.0% in routine therapy. The combined RR of H. pylori eradication rate was 1.186 (95% CI 1.118-1.257), and the combined RR of total side effects was 0.706 (95% CI 0.373-1.340). @*CONCLUSION@#Cultured milk products as adjunctive therapy is effective in improving the eradication rate during eradication therapy for H. pylori. However, it could not effectively decrease the risk of side effects.
Subject(s)
Animals , Humans , China , Combined Modality Therapy , Fermentation , Helicobacter Infections , Helicobacter pylori , Milk , Randomized Controlled Trials as TopicABSTRACT
<p><b>OBJECTIVE</b>To investigate relations between milk consumption and lactose intolerance (LI) in adults and to explore the effect of milk consumption on lactase activity.</p><p><b>METHODS</b>Total of 182 subjects aged 20-70 years were recruited and interviewed by questionnaires, and their accumulative cow's milk intake (AMI) was calculated. LI was evaluated by hydrogen breath test (HBT).</p><p><b>RESULTS</b>A negative correlation was found between AMI and severity of observed LI symptom (r=-0.2884; P<0.05). Binary logistic regression analysis showed a negative correlation between LI and duration and frequency of milk consumption (OR, 0.317 and 0.465, respectively; both P<0.05) and a positive correlation between LI and amount of milk consumed per sitting (OR, 6.337; P<0.05).</p><p><b>CONCLUSION</b>LI is related to various milk consumption behaviors. Most Chinese adults with LI may tolerate moderate milk consumption <160 mL.</p>
Subject(s)
Adult , Animals , Humans , Breath Tests , China , Epidemiology , Drinking Behavior , Hydrogen , Lactase , Metabolism , Lactose , Lactose Intolerance , Diagnosis , Epidemiology , Milk , PrevalenceABSTRACT
Objective To assess iron bioavailability of three iron-biofortified maizes using an in vitro digestion/ Caco-2 cell culture model. Method Three maize varieties rich in iron (Zhongtie 2, 3 and 4) and two maize varieties with lower content of iron (Zhengda 818 and 619) were pulverized, and the content of iron and phosphorus in each maize variety was measured. After digestion the iron bioavailability per g food was determined by using Caco-2 cell ferritin formation per mg cell protein as indicator. Results The iron content (33.250 mg/kg), iron bioavailability and the iron bioavailability per g food of Zhongtie 2 maize was the highest among all varieties, but its phosphorus content was lower. The maize varieties with the next higher iron bioavailability were Zhongtie 3 and Zhengda 818, and the lowest was Zhengda 619. Conclusion Zhongtie 2 maize contained the hightest content and bioavailability of iron,so it was optimal to use for the following human trials.
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In the present work the relative biological availability (RBA) of iron in the blood and the liver paste of swine has been studied by means of the hemoglobin regeneration technique in chickens. One day-old chickens were first made anemic on a low iron basal diet for 3 weeks, and were then divided into groups to which basal diets with various iron levels (10, 20, 40 ppm Fe) from different iron sources (ferrous sulfate reference standard, whole blood meal of swine and liver paste of swine) were subsequently fed respectively.After 16 days on test diets hemoglobin levels and hematocrit values in the blood of the chickens were measured. The chickens were then sacrificed for measurement of the iron concentration in their livers.Regression lines of each iron source were fitted by plotting final hemo- globin concentrations against the corresponding iron concentrations of the diet fed. Let the slope of the regression line from ferrous sulfate be 100, (i.e. relative bioavailability of ferrous sulfate), the RBA of the other 3 iron sources was as follows; processed whole blood meal of swine was 90.2; raw blood meal of swine 64.6; and the live paste of swine 78.4; but the differences between any two of them are statistically non-significant; i.e. their effects on hemoglobin regeneration are of the same extent.
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Objective: To assay the content and stability of vitamin C(VC) in serum by a simple, specific method. Methods: Serum samples were extracted by metaphosphoric acid, and analyzed on a column C 18 of HPLC with DAD detector at 234 nm. Mobile phase was 100 mmol/L KH 2PO 4, pH3.00. The flow rate was 1 ml/min. Results: Under this condition, the retention time of VC was 5.76 min. The within run and between run reproducibilities tests were 0.42% and 2.11% respectively, and the recovery rate ranged from 82% to 88.5%. Stored at -70 ℃ and compared to that of its metaphosphoric acid extract, serum VC content was stable only for two days, and then decreased rapidly, whereas it did not decrease in metaphosphoric acid extract until the fifth day. Conclusion: A simple, sensitive and reliable method of HPLC with DAD detector is established for measuring VC in either serum or fruit, such as orange juice. VC should be determined immediately or should be extracted with metaphosphoric acid as soon as possible, then the extracts are kept at -70 ℃, and analysed within 5 d.
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Objective: To investigate the effects of vitamin B 1, C and orange juice on human lung cancer cell proliferation in vitro. Methods: Vitamin C degradation in culture medium was evaluated. The methods included: methy thiazolyl tetrazolium (MTT) assay, colony forming assay and 3H TdR incorporation test. The final concentrations of factors in medium were: orange juice (vitamin C 30,60,120 ?g/ml), vitamin C(30,60,120 ?g/ml), vitamin B 1 (0.01,0.1,1 ?g/ml). Results: (1) In MTT test, orange juice at each level had significant inhibitory effect on the growth of lung cancer cells (P
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Seventy two diabetic subjects type Ⅱ were given konjac food for 65 days.The data analysed by multiple F-test indicated that the fasting blood glucosc(FBG), 2-h postprandial blood glucose (PBG) at the 30th and 65th day after consuming the food were significantly reduced (P = 0.001, P200mg%) decreased on the average by 51.8 and 84.6 mg% respectively, those with FBG-O 150-200 mg% by 24.1 and 68.7mg%, and those with FBG-0200 mg%).It could be concluded that konjac food was very useful in both prevention and treatment of hyperglycemia.
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In the present article the effects of animal blood and liver on the status of iron nutrition in preschool children were studied.71 apparently normal children in a kindergarten, aged 3-7 years, were selected for feeding trial. All of them were undergone physical examination to verify the absence of hookworm infestation and chronic diseases, but having a bit lower hemoglobin concentration (Hb
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Objective: To study the relationship between colonic microflora and lactose intolerance symptoms. Methods: 42 volunteers were chosen and divided into lactose intolerance(LI) and lactose malabsorption groups according to the 25 g lactose-challenge test and symptoms questionnaires. Fresh fecal samples were collected from the volunteers, and the fluorescence in situ hybridization (FISH) technique with group-specific 16S rRNA-targeted oligonucleotide probes was used to quantify the total bacterial and predominant groups of bacteria in fecal samples. The effect of milk-drinking habit on the number of Bifidobacteria was analyzed. Results: It showed that there was significant difference in the number of total bacteria and Bifidobacteria between lactose intolerance groups and malabsorption group(P
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With food containing konjac flour, a 45-day-long feeding trial on 110 old people suffering from hyperlipidemia was conducted to observe the effect of konjac food on lipid metabolism of human beings.The konjac flour was from Amorphophallus konjac, a kind of traditional food, with trace protein and very low energy, but rich in dietary fibre composed mainly of gluco-mannan.The results obtained were as follows;1.After having konjac flour 5g/day as a supplement of their regular meal for 45 days, concentration of TG, TC and LDLC in the serum of the experimental subjects decreased significantly (P
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Objective: To compare the methods of metabolic balance (MB) and fecal monitoring (FM) for evaluating the dietary zinc (Zn) absorption in Tibetan men. Methods: In 14 d field trial on 16 adult Tibetan men, capsules of carmine were given to mark the feces from D4 to D12, and samples of diet, water, feces and urine were collected during the period. In addition, 4.0 mg zinc tracer (enriched with 67Zn) and 1.0 mg recovery indicator ytterbium (Yb) were orally administrated to the subjects in the evening meal of the D5. The ratio of 67Zn/68Zn in fecal samples was determined by inductively coupled plasma mass spectrometry, and then the zinc absorption was calculated based on the principle of isotope dilution. Results: The dietary zinc absorption in Tibetan men was (23.8?3.9) % evaluated by MB and (21.4?4.3) % by FM with significant difference (by paired-samples t test) and linear correlation (Pearson). The unabsorbed zinc tracer and Yb had the similar behavior through the digestive tract, mostly excreted within5 d following the intake. Conclusion: In the 14d metabolic period, the dietary zinc absorption from MB was a little higher than that from FM. Using FM, the metabolic period can be shortened to 4-5 days according to excretion of Yb. Both methods suggested that the dietary zinc absorption in the adult Tibetan men was good.
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Objective: To study the absorption and utilization of dietary iron, zinc and calcium of male adults of Yi nationality, for basic information to prevent and cure diseases related to mineral deficiencies. Method: Twelve young men aged between 20 and 22 from the region lived by Yi nationality were studied in 14 d period with metabolic balance technique. At the beginning of study, their fasting blood hemoglobin and hematocrit, serum ferritin, plasma zinc and calcium concentration were deteimined. During the 4-12 d of the study, experimental diet, subsequent feces and urine samples were also collected for determining the contents of iron, zinc and calcium by flame atom absorption spectrum method, and then apparent absorption rate, apparent utilization rate and metabolic balance of these minerals were calculated. Results: The apparent absorption rate of iron, zinc and calcium was (15.8?6.2)%, (24.5?4.1)% and (32.3?9.7) % and the apparent utilization rate was (1.9?5.7)%, (12.8?4.3)% and (5.5?15.9)% respectively. The metabolic study showed iron balance, and zinc balance, but calcium balance was variable. Conclusion: The intakes of iron and zinc from the diet can meet the requirements, but calcium cannot. More intakes of foods rich in calcium are recommended for Yi nationality.
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Objective: To study the energy metabolism on adults with abnormal body weight. Method: Twenty four men aged 20-30 years old were chosen as the subjects whose BMI were more than 25 in obesity group, less than 18.5 in lean group and from 18.5 to 23 in normal group. K4b2 expiry monitor was used to study the energy metabolism of the subjects. Results: The total food intakes, the total energy intakes and the basal metabolism expenditure of the obese subjects were significantly higher than those in the normal and the lean group (P