ABSTRACT
Objective To investigate the effect of tripterygium glycosides(TG) combined with tanshinone Ⅱ A(Tan Ⅱ A) on the level of tumor necrosis factor-α(TNF-α) ,yon willebrand factor(vWF) in serum in adjuvant ar-thritis(AA) mice. Methods Animal model of adjuvant arthritis was indwecd,after 3 weeks administration the mice were kiued and get serum. TNF-α, vWF were assayed by ELISA. Results TNF-α: model group and Tan Ⅱ A group are significantly higher than control group,TG + TanⅡ A group are lower than the other groups,there is no significant difference between control group, MTX group,TG group; vWF: model group have the highest vWF level, has significant difference between control group ,MTX group, TG group, TG + Tan Ⅱ A group and Tan Ⅱ A group, TG + Tan Ⅱ A group are lower than the other group. Conclusion TG + Tan Ⅱ A can improve cardiovascular damage of rheumatoid arthritis by means of inhibiting inflammatory factor expression.
ABSTRACT
Objective To explore the mechanism of apeptosis in rheumatoid arthritis synoviocyte induced by dihydroartemisinin. Methods Synovial tissues were cut from rheumatoid arthritis patients when who was under knee prosthesis. Apoptosis was detected with flow cytometry. Western blot was performed to assess ser473-phosphorylated Akt. EMSA (electrophoretic mobility shift assay) was used to ana-lyze NF-κB activation. Results Dihydroartemisin can induce apoptosis in rheumatoid arthritis synoviocyte in a dose-dependent manor from 2.5μmol/L to 10μmol/L. Rheumatoid Arthritis synoviocyte cultured with dihydroartemisinin in 5μmol/L or 10μmoL/L can significantly in-hibit serine 473 phosphorylation in Akt and activation of NF-κB. Conclusion Dihydroartemisinin can induce apoptosis in rheumatoid arthri-tis synoviocyte through Akt signal pathway.
ABSTRACT
AIM: To explore the role of synovium macrophage of adjuvant arthritis (AA) rats in articular damage. METHODS: AA was induced in female Wistar rats by means of intradermal injection with complete Freud's adjuvant. X-ray on hind paws of AA rats was took at different time points and radiograph index (RI) was then evaluated. Synovium macrophages were detected through immunohistochemistry with ED 1 monoclonal antibody. The correlations between RI and synovial lining layer count/synovium macrophage count were also analysed. RESULTS: Synovial lining layer count, both macrophage count in synovial lining layer and sublining layer were positively correlated with RI. CONCLUSION: Synovium macrophages of AA rats may play an important role in articular damage.