ABSTRACT
Aim: To study multiple antimicrobial resistances in Vibrio spp. isolated from river and aquaculture water sources in Imo State Nigeria. Methodology: A total of 157 Vibrio isolates from river and aquaculture water sources were analysed for multiple antimicrobial resistance during a 6 month period. Antimicrobial resistance profile was determined by the Kirby-Bauer technique, while the phenotypic expression of β-lactamase production was performed by the double disk diffusion method. PCR was used to screen isolates for the presence of β-lactamase resistance genes. Results: The isolates from river water expressed high resistance rates (81.3 to 97.8%) to the following antimicrobials: mezlocillin, doxycycline, tetracycline, carbenicillin and ampicillin, while resistance rate to kanamycin was moderate at 40.9%. Resistance rates for the aquaculture water Isolates were also high for the same antibiotics as the river water isolates, while resistance rate to kanamycin was low to moderate at 32.8%. Phenotypic screening of isolates for ESβL production showed the isolates were resistant to β-lactam antimicrobials and the β-lactamase inhibitor of amoxicillin/clavulnic acid combination. Gel electrophoresis of PCR products showed amplification for blaTEM of size 964bp. Conclusion: Results showed the presence of highly resistant Vibrio isolates from the sampled environmental sources. The presence of resistance markers among the isolates in this study infers that they could be agents of transfer of resistance to other bacterial pathogens found in river and aquaculture water.
ABSTRACT
Background: In Nigeria; concerns on the quality and financing of health-care delivery especially in the public sector have initiated reforms including support for public-private partnerships (PPP) at the Federal Ministry of Health. Likewise; Enugu State has developed a draft policy on PPP since 2005. However; non-validation and non-implementation of this policy might have led to loss of interest in the partnership. Aim: The aim of this study was to provide evidence for planning the implementation of PPP in Enugu State health system via a multi-sectoral identification of challenges; constraints and prospects. Subjects and Methods: Pre-tested questionnaires were administered to 466 respondents (251 health workers and 215 community members); selected by multi-stage sampling method from nine Local Government Areas of Enugu State; Nigeria; over a study period of April 2011 to September 2011. Data from the questionnaires were collated manually and quantitative data analyzed using SPSS version 15 (Chicago; IL; USA). Results: Only 159 (34.1; 159/466) of all respondents actually understood the meaning of PPP though 251 (53.9) of them had claimed knowledge of the concept. This actual understanding was higher among health workers (57.8; 145/251) when compared with the community members (6.5; 14/215) (P 0.001). Post-PPP enlightenment reviews showed a more desire for PPP implementation among private health-care workers (89.4; 101/113) and community leaders/members (55.4; 119/215). Conclusion: PPP in health-care delivery in Enugu State is feasible with massive awareness; elaborate stakeholder's engagements and well-structured policy before implementation. A critical challenge will be to convince the public sector workers who are the anticipated partners to accept and support private sector participation