ABSTRACT
Prevalences of human herpesvirus 6 (HHV-6) and human herpesvirus 7 (HHV-7) DNA were investigated in normal Thai population. Peripheral blood mononuclear cells (PBMC) and saliva were collected from 238 healthy adults in five provinces which might be a representative of each part of the country, and 120 normal children in one province. Prevalences of HHV-6 DNA PBMC were 45.5-74.3% in adults and 78.3% in children, and in saliva, very low prevalences were detected; 5.7-8.6% in adults and 15.0% in children, respectively. Additionally, all HHV-6 DNA detected in this study were variant B. Comparingly to those of HHV-7 DNA, the prevalences were significantly higher than those of HHV-6, ie, 82.9-91.4% in PBMC of adults, 85% in PBMC of children, 84.8-89.0% in saliva of adults and 92.5% in saliva of children. HHV-6 and HHV-7 isolation from saliva specimens were also performed. No HHV-6 could be isolated from any samples, whereas, in the present study, HHV-7 could be isolated as 90.0% from children and as 20.0-54.5% from adults.
Subject(s)
Adolescent , Adult , Age Distribution , Blotting, Southern , Child , Child, Preschool , Herpesviridae Infections/epidemiology , Herpesvirus 6, Human , Herpesvirus 7, Human , Humans , Middle Aged , Polymerase Chain Reaction , Prevalence , Thailand/epidemiologyABSTRACT
The DNAs of 17 isolates of varicella-zoster virus (VZV) obtained from 17 Thai individuals with normal varicella or zoster infections (no underlying diseases) were compared by restriction endonuclease analysis using BglI, PstI, EcoRI, SmaI and BamHI. The DNA of the Japanese strain, Kawaguchi, was also conducted as a reference DNA. All of virus isolates were confirmed for existence of VZV and VZV-DNA by immunofluorescent test and DNA-hybridization, respectively. Almost all of the Thai epidemiologically unrelated isolates and the Kawaguchi strain could be individually differentiated using BglI, PstI, and EcoRI. The other two isolates were identical in restriction profiles even after five endonuclease digestions which SmaI and BamHI were the two more enzymes used, therefore, they could be discriminated totally into 16 strains from overall 17 isolates. These findings demonstrate the strain variation of wild-type varicella-zoster viruses isolated in Thailand.