ABSTRACT
<p><b>OBJECTIVE</b>To investigate the protective effect and mechanism of Xingnaojing(Traditional Chinese Medicine) injection on brain injury in rats.</p><p><b>METHODS</b>Sixty-three healthy adult male SD rats were randomly divided into 3 groups (n = 21): sham operation group, model group, xingnaojing group. The model of traumatic brain injury model group and Xingnaojing group used the free fall impact injury method, the sham operation group underwent craniotomy, did not cause brain damage. Xingnaojing group in rats after 10 min by tail vein injection Xingnaojing injection 10 ml/(kg x d), model group and sham operation group were intravenously injected with 0.9% sodium chloride solution, three groups were administered continuously for 7 days. At administration of the seventh days compared the S-100B protein in the serum and neuro specific enolase (NSE) level, the water content of brain tissue, serum superoxide dismutase (SOD), malondialdehyde (MDA) and glutathione peroxidase (GSH-Px) content, and neurological function of rats among groups.</p><p><b>RESULTS</b>Compared with the sham operation group, the nerve defect, brain water content, MDA, S100B protein and NSE levels were obvigusly increased in Xingnaojing group and model group; SOD, GSH-Px content decreased significantly; In Xingnaojing group nerve impairment and brain moisture were significantly lower than those of model group, the serum MDA, S-100B protein and NSE levels were significantly lower than those in the model group, the SOD, GSH-Px activity was significantly higher than that in the model group.</p><p><b>CONCLUSION</b>Xingnaojing injection has protective effects on rat brain injury, and its mechanism may be related to reduce brain edema after traumatic brain injury and inhibit the reaction of oxygen free radical, protect nerve cells.</p>
Subject(s)
Animals , Male , Rats , Brain Injuries , Metabolism , Disease Models, Animal , Drugs, Chinese Herbal , Pharmacology , Glutathione Peroxidase , Metabolism , Malondialdehyde , Metabolism , Phosphopyruvate Hydratase , Metabolism , Rats, Sprague-Dawley , S100 Calcium Binding Protein beta Subunit , Blood , S100 Proteins , Metabolism , Superoxide Dismutase , MetabolismABSTRACT
<p><b>OBJECTIVE</b>To explore the role and function of stromal cell-derived factor-1 (SDF-1) in stem cells migrating into injured brain area.</p><p><b>METHODS</b>Rat-derived nerve stem cells (NSCs) were isolated and cultured routinely. Transwell system was used to observe the migration ability of NSCs into injured nerve cells. Immunocytochemistry was used to explore the expression of chemotactic factor receptor-4 (CXCR-4) in NSCs. In vivo, we applied immunofluorescence technique to observe the migration of NSCs into injured brain area. Immunofluorescence technique and Western blotting were used to test expression level of SDF-1. After AMD3100 (a special chemical blocker) blocking CXCR-4, the migration ability of NSCs was tested in vivo and in vitro, respectively.</p><p><b>RESULTS</b>NSCs displayed specific tropism for injured nerve cells or traumatic brain area in vivo and in vitro. The expression level of SDF-1 in traumatic brain area increased remarkably and the expression level of CXCR-4 in the NSCs increased simultaneously. After AMD3100 blocking the expression of CXCR-4, the migration ability of NSCs decreased significantly both in vivo and in vitro.</p><p><b>CONCLUSIONS</b>SDF-1 may play a key role in stem cells migrating into injured brain area through specially combining with CXCR-4.</p>