ABSTRACT
Dopamine is one of the bioactive amines which regulate many physiological functions. In order to study its localization and quantification in tissues with immunohistochemistry and radioimmunoassay, authors immunised New Zealand rabbits with dopamine-BSA (bovine serum albumin) complex and succeeded in the production of the specific antiserum. The antiserum was tested on routine paraffin sections of Bouin's fixed guinea-pig digestive tract with the ABC technique. The titre of the antiserum was 1:1000-1:2000. Results of substitution, absorption and cross-absorption tests proved that the positive staining obtained with the antiserum demonstrated the specific immunoreaction between the antibody to dopamine and the antigen in the tissues.
ABSTRACT
Localization and quantitation of GnRH, somatostatin (SS), and ?-endorphin (?-EP) in human placentalvilla were studied using immunogold-silver staining method. GnRH and SS immunoreactive positive substance existed in the cytotrophoblasts of many placental villi and in syncytiotrophoblasts of few placental villi, ?-EP immunoreactive positive substance localized in syncytiotrophoblasts of many placental villi and in cytotrophoblasts of few placental villi. These results suggest that the cytotrophoblasts and syncytiotrophoblasts are able to synthesize these three kinds of regulatory peptides, however, SS and GnRH may be synthesized mainly in the cytotrophoblasts, ?-EP mainly be synthesized in the syncytiotrophoblasts. The amount of GnRH and ?-EP in placenta villi show obvious change with progress of pregnancy. This change show a negative relationship between the GnRH and ?-EP, which suggested that there may be a functional reciprocal inhibition between the GnRH and ?-EP.
ABSTRACT
The distribution of dopamine-immunoreactive endocrine cells and nerves in the digestive tract of guinea-pigs was examined with the ABC immunostaining technique. Dopamine-immunoreactive endocrine cells were observed in the pyloric antrum, small intestine and colon. The highest density of the positive ceils was found in the duodenum. From the jejunum to the colon the number of immunoreactive cells gradually decreased. A similar population of the positive cells was seen in the antrum and colon. Dopamine-immunoreactive nerve fibres were mainly located in the lamina propria and submucosa of the digestive tract. There were more positive fibres in the small intestine than in the stomach and colon. The dense networks of immunoreactive fibres were demonstrated in the villi. Some positive nerve fibres penetrated into the superficial and glandular epithelia. This representation indicated that the epithelial cells were directly innervated with the dopamine containing nerves.