ABSTRACT
BACKGROUND: The aim of this experimental study was to investigate the expression of nNOS following ischemia/reperfusion injury in rat spinal cord. METHODS: Spraque-Dawley rats (250-300 gm) were classified into two groups according to experimental methods. Control group (n = 5) received sham operation. Ischemia in experimental group (n = 5) was induced by clamping the abdominal aorta just below the left renal artery and the abdominal aorta just above the aortic bifurcation for 20 minutes followed by 20 minutes reperfusion. The spinal cord was obtained 7 days after operation. The expression of nNOS was examined in gray and white matter with confocal microscope and Image Analyzer. RESULTS: There is no difference in nNOS expression of nNOS between gray and white matter in control group. Comparing to control group, experimental group showed higher levels of nNOS expression (P < 0.05). CONCLUSIONS: nNOS expression increased in the rat spinal cord after ischemia/reperfusion injury. We suggest that overproduction of NO may play a role in progressive nerve cell damage.
Subject(s)
Animals , Rats , Aorta, Abdominal , Constriction , Ischemia , Neurons , Nitric Oxide Synthase Type I , Renal Artery , Reperfusion , Spinal CordABSTRACT
Airway management is important during general anesthesia. Difficulty with a direct laryngoscopy can be routinely successfully managed using a laryngeal mask airway. However, a laryngeal mask airway may not be suitable for the patients having a small oral cavity volume or narrow interincisor distance. A 19-year-old man, scheduled for right clavicular fracture had no specific underlying medical problems. However, he had difficultly opening his mouth, and was Mallampati class IV. Also, he had difficultly in extending his neck. Airway management was successfully performed using a laryngeal tube instead of a laryngeal mask airway. The patient was successfully managed, without complications, until the end of the surgery.
Subject(s)
Humans , Young Adult , Airway Management , Anesthesia, General , Intubation , Laryngeal Masks , Laryngoscopy , Mouth , NeckABSTRACT
No abstract available.
Subject(s)
Female , Pregnancy , Chorion , Chorionic Villi Sampling , Chorionic VilliABSTRACT
Human papillomavirus(HPV) has implicated in the development of cervical cancer. Several studies has suggested a strong correlation between HPV 16, 18 and cervical intraepithelial neoplasia(CIN). For detecting and typing HPV DNA in cervical tissues, recently the chemiluminescent molecular hybridization assay method has been widely used. This study was performed to determine the usefulness of hybrid capture assay for detecting high-risk HPV in cervical epithelial cells, and to compare the correlation among cervical cytology, biopsy finding and HPV infection, and to determine whether the addition of the hybrid capture assay to cytologic test would improve the ability to identify significant lesions. This study included 267 patients who visited the colposcopic clinic of the department of obstetrics and gynecology, Chonbuk University Hospital from May, 1997 to October 199S. Pap smears hybrid capture assays, and colposcopically directed biopsy were performed concurrently on al1 women. The results obtained were as follow; l. Using hybrid capture assay, the detection rate of high-risk HPV of all patients was 37.1%(99/267). There was no statistical significance in the detection rate of HPV between the age groups. 2. The false negative rate of Pap Smear was 53.3% and showed significant discrepancies between the cytologic and histologic diagnosis. 3. According to the cytologic diagnosis, the detection rates of high-risk HPV were 7.1% in normal, 25.3% in ASCUS or LSIL, and 61.6% in HSIL. In each cytologic group, the patients who had positive results for high-risk HPV showed higher incidence rate of high grade lesions than those with negative results(P<0.05). 4. According to the histologic diagnosis, the detection rates of high-risk HPV were 0.1% in normal, 11.1% in CIN I, and 72.7% in CIN II or CIN III. In each histologic group, the patients who had positive results for high-risk HPV showed higher incidence rate of high grade lesions than those with negative results(P<0.05). 5. According to the comparison of histologic diagnosis between positive and negative results of high-risk HPV test due to each cytology, there was no statistical significance in the incidence rate of cervical neoplasia, Above results suggest that high-risk HPV test using hybrid capture assay may be a useful method in supplement the pitfalls of cervical cytology. This test might also have prognostic value in the management of patients with CIN.