Identification and isolation of ATP transport protein in mycobacillin sensitive Aspergillus niger.

The temperature sensitive release and uptake of ATP through the Aspergillus niger G3Br membrane vesicles followed saturation kinetics. Both the processes which occurred in the absence of mycobacillin were greatly enhanced by its presence. Liposomes prepared with antifilipin sterol and lipid showed the release and uptake of ATP in the presence of filipin, but no such uptake and release was seen with antimycobacillin sterol and lipid in the presence of mycobacillin. However the liposomes supplemented with Aspergillus niger membranes protein (s) showed the release and uptake of ATP, implicating membrane protein as a carrier in the transport process.