ABSTRACT
Aims: The main objective of this work was to observe the analgesic activity of Manilkara zapota (leaves) on mice. Study Design: The Present study was designed to observe pharmacological activities of the crude extracts of the plant Manilkara zapota (leaves). The study protocol consisted of Cold extraction at room temperature of the whole plant with distilled methanol. Afterwards, Filtration of the crude Methanolic and Petroleum ether extracts by using the Markin cotton cloth and subsequently through the filter paper and solvent evaporation. Finally, screening of analgesic activity of crude extracts on Swiss Albino mice. Place and Duration of Study: School of Science and Engineering, Department of Pharmacy, Southeast University, Bangladesh, January 2011 to August 2012. Methodology: The analgesic activity was investigated for its peripheral pharmacological actions by using acetic acid-induced writhing test in mice. Results: The Methanolic and Petroleum Ether extracts, at the dose of 200 mg/kg body weight, displayed 96.82% & 94.27% pain inhibition which was significant (p<0.001) compared to control. These results indicate that the extracts possess significant analgesic activity. Conclusion: This study suggests that the Methanolic and Petroleum Ether extract of Manilkara zapota leaves have analgesic activity in a dose dependent manner which supports it’s as an analgesic drug in folk medicine.