ABSTRACT
Objective To investigate the relationship between gene polymorphism of interleukin (IL) -1 and peri-implantitis. Methods A case-control experimental design was used in this study. Moreover, using a bioinformatics analysis, we selected three tagged single nucleotide polymorphisms (SNP) suitable for the Chinese population. Patients with peri-implantitis (n = 116) and 87 patients without peri-implantitis were included. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to tag SNP of IL-1α, IL-1β, and IL-1γn. Results The rs2856838 locus of the AA genotype of IL-1α was associated with a higher risk of implant periodontitis than the GG genotype. There was no statistical difference between the two groups with regard to the other SNP. Conclusion The AA genotype of rs2856838 is associated with periprosthetic inflammation.
ABSTRACT
BACKGROUND: It has been reported that 26-1 ferrite stainless steel has excellent biological characteristics, but the report of its biocompatibility is rare, especially the evaluation of biocompatibility by apoptosis mechanism. OBJECTIVE: To study the pattern of call death induced by magnetic-attached 26-1 ferrite stainless steel and to evaluate whether 26-1 ferrite stainless steal meats the biocompatibility in clinical application. DESIGN, TIME AND SETTING: A contrast observational study at the level of molecular biology was performed at Central Laboratory of China Medical University from June 2004 to June 2005.MATERIALS: 26-1 ferrite stainless steel (Fe - 26% Cr - 1% Mo), Tilite [70% Ni - 16% Cr - (4%-6%) Ti - ether alloy elements] and Co-Cr alloy (Co - 30% Cr - 5% Mo) were used in this study. Embed and cast metal materials then were cut into roll discs whose diameters were 12 mm.METHODS: ① The fibroblast L929 cells were co-cultured with the three materials, while cells in the negative control group were cultured in RPMI1640 media. At 18 and 24 hours after culture, types of cell death were quantitatively detected using fluorescein isothiocyanate and propidium iodide (PI) staining. ② Apoptosis rate and cycle distribution of L929 cells were detected using flow cytometry and Pl staining at 24 hours after respectively culturing in 25%, 50%, and 100% leaching liquor. MAIN OUTCOME MEASURES: Ratio between apoptosis and necrosis of L929 cells induced by different materials; apoptotic rate and cycle distribution of L929 cells.RESULTS: All the three materials could decrease the survival rate of L929 cells: in particular, there was no significant difference in necrosis rate between different concentration material groups and negative control group, suggesting that call apoptosis was the major factor to induce cytopathic effect, but there was no significant difference between the three material groups. Apoptosis of L929 cells indicated a great fashion of time- and concentration-dependence. There was no significant difference in apoptosis rate of L929 cells betwean the three material groups.CONCLUSION: Cell apoptosis is a major pattern induced by 26-1 ferrite stainless steel which meets the biocompatibility as a clinical material and can be safely applied in dinic.
ABSTRACT
Objective To investigate the possibility of occlusal pad in treating disorder of temporomandibular joint syndrome.Methods We treated 25 patients with disorder of temporomandibular joint syndrome using occlusal pad made of elastic resin,and evaluated the effects.Results Total effective rate was 96% .Clicking and pain dissappeared in all patients.Conclusion Occlusal pad made of elastic resin could be used for treating disorder of temporomandibular joint syndrome in clinic.