ABSTRACT
BACKGROUND: In recent researches the concentration of S100 proteins and neuron-specific enolase have been proved positively correlated with the degree of brain injury, and can be used for assessing the degree and prognosis of brain injury. But whether the level of serum S100 proteins and phosphopyruvate hydratase can predict the role of glucocorticosteroid in the treatment of CNS damage is unclear.OBJECTIVE: To observe the changes of serum S100 proteins and phosphopyruvate hydratase level, aiming to explore the role of methylprednisolone in the treatment of craniocerebral inju ryin rats.DESIGN: Randomized controlled study.SETTING: The First Affiliated Hospital of Sun Yat-sen University.MATERIALS: This study was carried out at the Neurological Research Institute of Guangzhou Medical College in September 2003. Totally 72 SD rats were enrolled in this study.METHODS: Totally 72 SD rats were randomly divided into three groups,namely normal group of 8 rats, comparison group and treatment group of 32 rats respectively, the latter two groups were then subdivided into post-trautablishment: Brain contusion and laceration was made in rats according to Feeney's method, rats in normal group were only subjected to craniotomy group, rats were given intraperitoneal injection of methylprednisolone by dosage of 30 mg/kg instantly after injury, which replace by physical saline of the same dosage in comparison group. Rats in normal group were not given intraperitoneal injection, blood specimen was obtained instantly after put to death by cutting off head; in comparison group and treatment group,blood sample was obtained from rat at post-traumatic 1, 6, 12 and 24 hours and centrifuged for collecting superuatant, the level of serum S100 proteins and phosphopyruvate hydratase was detected by using ELISA method.RESULTS: Totally 72 rats were enrolled in this experiment and all regroup was (0.35±0.029) μg/L. The difference of serum S100 proteins was not obvious between normal group and treatment group at post-traumatic 24 hours, but the level of S100 proteins in treatment group and comparison group was significantly higher than normal group (P ＜ 0.01 or P ＜ 0.05);moreover in contrast with comparison group, the level of S100 proteins was found obviously lower in treatment group at post-traumatic 6, 12 hours and hydratase was (8.35±1.01) μg/L in normal group, the difference of serum phosphopyruvate hydratase level between comparison group and treatment group was higher than normal group, displaying statistical significance at all post-traumatic time points (P ＜ 0.01), except for not obvious difference between comparison group at post-traumatic 24 hours and treatment group.While comparing to comparison group, the level of serum phosphopyruvate hydratase was found obviously lower in treatment group at post-traumatic 1,6 and 12 hours time point (P ＜ 0.01 or P ＜ 0.05).CONCLUSION: The level of serum S100 proteins and phosphopyruvate hydratase can be used as predictors for the degree of brain injury in rats, in addition methylprednisolone might exert therapeutic effect on traumatic brain.
ABSTRACT
Objective To observe the role of methylprednisolone in treatment of brain injuries through comparing the changes of serum S 100B protein and neuron specific enolase (NSE) levels. Methods Seventy two rats with brain contusion made by frontoparietal bone windows plasty with extradural hitting were divided into three groups: control group ( n =32), normal group ( n =8) and treatment group ( n =32) that were subdivided into groups 1, 6, 12 and 24 hours after injury. The levels of S 100B protein and NSE were measured at different time points after hitting by ELISA. Results ①The levels of serum S 100B protein and NSE was (0.35?0.03) ?g/L and (8.35?1.01) ?g/L, respectively in normal group. The levels of serum S 100 B protein and NSE in control group and treatment group (6 24 hours post injury) were higher than those in normal group ( P