MiR-33a suppresses breast cancer cell proliferation and metastasis by targeting ADAM9 and ROS1

MicroRNAs (miRNAs) are small noncoding RNAs that have a pivotal role in the post-transcriptional regulation of gene expression by sequence-specifically targeting multiple mRNAs. Although miR-33a was recently reported to play an important role in lipid homeostasis, atherosclerosis, and hepatic fibrosis, the functions of miR-33a in tumor progression and metastasis are largely unknown. Here, we found that downregulated miR-33a in breast cancer tissues correlates with lymph node metastasis. MiR-33a expression is significantly lower in the highly metastatic breast cancer cell lines than the noncancerous breast epithelial cells and non-metastatic breast cancer cells. Moreover, the overexpression of miR-33a in metastatic breast cancer cells remarkably decreases cell proliferation and invasion in vitro and significantly inhibits tumor growth and lung metastasis in vivo, whereas its knockdown in non-metastatic breast cancer cells significantly enhances cell proliferation and invasion in vitro and promotes tumor growth and lung metastasis in vivo. Combining bioinformatics prediction and biochemical analyses, we showed that ADAM9 and ROS1 are direct downstream targets of miR-33a. These findings identified miR-33a as a negative regulator of breast cancer cell proliferation and metastasis.

Treatment of Tossy III acromioclavicular joint dislocation：biomechanical change of anchor-reconstructed coracoclavicular ligament / 中国组织工程研究

BACKGROUND:The commonly used methods of repairing the acromioclavicular joint dislocation are Kirschner wire tension band internal fixation, clavicular hook internal fixation and suture anchor internal fixation. Each method has their advantages and disadvantages. Both Kirschner wire and clavicular hook require secondary surgery to remove the internal fixators, and their design is not based on the biomechanical principle. OBJECTIVE:To reconstruct coracoclavicular ligament using anchor and perform biomechanical tests. METHODS:Ten shoulder antisepsis specimens were numbered and each specimen was tested by three trials. (1) Stretching coracoclavicular ligament: Specimens were fixed on the biomechanical machine and were tensile until the ligament was ruptured, the maximum failure load was recorded. (2) Reconstruction of coracoclavicular ligament using an anchor: Coracoclavicular ligment was reconstructed using an anchor, then specimens were fixed on the biomechanical machine and were tensile until the ligament was ruptured, the maximum failure load was recorded. (3) Reconstruction of coracoclavicular ligament using two anchors: Coracoclavicular ligment was reconstructed using two anchors, then specimens were fixed on the biomechanical machine and were tensile until the ligament was ruptured, the maximum failure load was recorded. RESULTS AND CONCLUSION:Coracoclavicular ligament fracture load was (413.0±123.48) N; the failure load was (345.1±111.23) N in an anchor group and 465.3±100.64 N in two anchors group. When coracoclavicular ligament is reconstructed using an anchor, the stress is concentrated under external forces, which is prone to the extraction of anchor and the failure of surgery, biomechanical data showed that the fracture load is lower than the coracoclavicular ligament, so the reconstruction is not reliable; when coracoclavicular ligament is reconstructed using two anchors, the stress is dispersed, which meet the biological features and the biomechanical data are ideal.

Analysis of the CT signs and the clinical relevant factors of degenerative osteoarthritis of atlanto-odontoid joint / 中国医师进修杂志

Objective To analyze the CT signs and the clinical relevant factors of degenerative osteoarthritis of athnto-odontoid joint.Methods The CT signs and the clinical information of 214 patients received CT scans of skull from June 2011 to April 2012 were analyzed retrospectively.Results One hundred and twenty patients were diagnosed with degenerative osteoarthritis of atlanto-odontoid joint,while 67 of them having subaxial degenerative osteoarthritis at the same time.Forty-three patients had a history of chronic cervical and occipital pain.95.83％ (115/120) patients showed osteophytes in CT scans,while 44.17％ (53/1 20) showed calcification around dens and 24.17％ (29/120) showed joint space obliteration.The incidence rate increased with age.Seventy-seven patients [64.17％(77/120)] appeared cervical and occipital pain.Conclusions Degenerative osteoarthritis of atlanto-odontoid joint shows hyperostosis,osteophytes at the edge of joint,joint space obliteration and calcification around dens in the CT scans.The incidence rate is increased with age and correlated with cervical and occipital pain.

Analysis of influence factors of long-term efficacy of different fusion combined with pedicle fixation for lunbar spondylolysis / 中国医师进修杂志

Objective To analyze the influence factors of long-term efficacy of different fusion combined with pedicle fixation for lumbar spondylolysis.Methods The surgical and postoperative follow-up data of 196 cases with lumbar spondylolysis were analyzed retrospectively.Age,gender,body mass index ( BMI ),disease course,preoperative Japanese orthopaedic association (JOA) score,fusion segments,fusion method,immediate postoperative recovery rate and postoperative functional exercise,which might affectlong-term operation efficacy,were selected to undergo single-factor analysis and multivariate regression analysis.Results All the patients were followed up for 5-9 years and 5.4 years for average,163 cases got excellent or good curative effect,the rate of excellent and good effect was 83.2％( 163/196);33 cases got poor efficacy.There was no cerebrospinal membrane tear,cerebrospinal fluid leakage,infection,fracture fixation and other complications happened after operation.Single-factor analysis showed that disease course,BMI,preoperative JOA score and postoperative functional exercise had obvious relation with long-term efficacy (P ＜ 0.05 ) ;while age,fusion method,fusion segments,immediate postoperative recovery rate and gender had no significant relation with long-term efficacy (P ＞ 0.05).Multivariate regression analysis showed that disease course,preoperative JOA score,postoperative functional exercise were important factors influencing long-term efficacy (OR =1.423,2.089,1.320,P=0.023,0.012,0.034).Conclusions Disease course,preoperative JOA score,postoperative functional exercise are important factors influencing long-term efficacy of surgery for patients with lumbar spondylolysis.

The study of combined treatment for advanced endometrium carcinoma──A report of 45 cases / 中国癌症杂志

Purpose:To study the effect of combined treatment on advanced endometrium carcinoma. Methods:45 cases of stage Ⅲ endometrium carcinoma with combined treatment were retrospectively reviewed. Operation was the most used mode in the combined treatment. Depending on the pathology, metastatic site etc. 31 cases were treated in combination with chemotherapy, 11 cases with operation and radiotherapy, 3 cases with radiotherapy. All cases had hormonotherapy. The multi-drug chemotherapy was dominated by platinum agents. CFP 17 cases (54.83%),EFP 5 cases (16.12%), CAP 4 cases (12 90%), CP 3 cases(9.68%), others 2 cases. Survival analysis were done by Kaplan-Meier model of SPSS 8.0. Results:15 cases of Ⅲ stage were treated by operation and chemotherapy, 16 cases by operation chemotherapy and radiotherapy, 11 cases by operation and radiotherapy. The 2-year survival rate was 69.23%,78.97%, 70.71%. There was no difference in statistics. The 5-year survival rate was 30.29%,47.38%,14.14%. Chemotherapy in the combined treatment partly improved long-term cure P