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Aim To explore the role of the silent information regulator (silent information regulation 1, SIRTl)-mediated apoptotic pathway in the protection of sevoflurane postconditioning in hippocampal neuronal injury of mice induced by hemorrhagic shock resuscitation (HSR). Methods A mouse model of HSR was established. The male C57BL/6J mice were randomly divided into; sham operation group (Sham group), HSR group (Shock group), sevoflurane treatment group (Sevo group), sevoflurane combined with SIRTl specific inhibitor treatment group (EX527 + Sevo group) and EX527 treatment group (EX527 group). The volume of cerebral infarction was detected by TTC staining method. The changes of hippocampal nerve cells in each group of mice were detected by TUNEL staining method. The learning and memory abilities were detected by Morris water maze test. The expression of SIRTl and apoptosis-related protein levels were delected by Western blot analysis. Results The latency of the model mice reaching the platform in Morris water maze test was prolonged, while the movement distance in the target quadrant was similarly reduced. Besides, the cerebral infarction volume remarkably increased. The number of TUNEL staining positive cells increased, and the expression of SIRTl and Bel-2 decreased while the pro-apoptosis protein Bax, and cleaved-caspase 3 expression level increased; sevoflurane treatment improved nerve injury in HSR. After combined treatment with sevoflurane and SIRTl inhibitor EX527, the protective effect of sevoflurane was attenuated on nerve injury in HSR. Conclusion Sevoflurane may play a protective role against hippocampal neuronal injury caused by HSR mediated by SIRTl apoptosis-related pathway.
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Objective To investigate the effect of the bletilla striata polysaccharide on the expression of ovarian vascular endothelial growth factor(VEGF) and serum malondialdehyde(MDA)and endometrial morphology in rat during perimenopause period.Methods The animal model of perimenopause rats was established by unforced aging.A total of 40 female SD rats(12~14 months old)were randomly divid-ed into four groups:Chinese medicine low(0.5 g/kg),medium(1 g/kg),high(2 g/kg) dosage group and the elder model group.The rats were given with corresponding drugs for 8 weeks.The content changes of ovarian VEGF protein was detected by western blot;serum MDA con-tent was detected by thiobarbituric acid;the morphology of endometrium in rats was observed by HE staining.Results Eight weeks later, compared with the elder model group,the VEGF protein expression in Chinese medicine low,medium,high dosage group increased,the serum MDA content in Chinese medicine low,medium,high,dosage group reduced,the differences were statistically significant( P<0.01),with dose dependence.Endometrial thickening and glandular increasing with the increase of dose.Conclusion Bletilla striata polysaccharide can upregulate the expression of VEGF protein,and down regulate the level of serum MDA,which improves the uterine function so as to delay the process of perimenopause in rats.
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<p><b>OBJECTIVE</b>To explore the clinical effects of cervical posterior pedicle screw internal fixation and interbody fusion for the treatment of atlanto-axial vertebral instability fractures.</p><p><b>METHODS</b>From July 2008 to July 2013, 21 patients with atlanto-axial vertebral instability fractures were treated with vertebral pedicle screw internal fixation and interbody fusion through posterior approach. There were 14 males and 7 females, aged from 20 to 55 years old with an average of 32 years. Lifted and guided the atlanto-axial vertebral pedicle screw to reduce atlanto-axial vertebral displacement.</p><p><b>RESULTS</b>All patients were followed up from 6 to 24 months with an average of 12.5 months. Wounds got healed without complication of infection. Clinical symptoms were relieved at 6 months after operation. According to ASIA score standard to assess at 6 months after operation, the items of motion, light touch and needle score had obviously improved, and respectively were 99.45 ±0.27, 111.09 ± 0.47,111.11 ± 0.58. VAS and NDI scores also had obviously improved, and respectively were 1.04 ± 0.38 and 12.56 ± 2.24. Imaging examinations showed internal fixation locations were good, without atlanto-axial joint instability.</p><p><b>CONCLUSION</b>Cervical posterior pedicle screw internal fixation and interbody fusion can effectively restore the stability of atlanto-axial joint, reduce complication and obtain satisfactory effects.</p>
Subject(s)
Adult , Female , Humans , Male , Middle Aged , Young Adult , Atlanto-Axial Joint , Wounds and Injuries , General Surgery , Fracture Fixation, Internal , Methods , Joint Instability , General Surgery , Pedicle Screws , Spinal Fractures , General Surgery , Spinal Fusion , MethodsABSTRACT
<p><b>OBJECTIVE</b>To investigate clinical efficacy and feasibility of double-plating fixation via anteriormiddle approach in treating type C3 distal femoral fractures.</p><p><b>METHODS</b>From August 2008 to August 2011, 12 cases with type C3 distal femoral fractures were treated, including 5 open fractures and 7 closed fractures. Among them, there were 8 males, 4 females with an average of 40 years (ranged, 25 to 55 years). There were 7 in left side, 5 in right side. Nine cases were caused by car accident, 3 cases by falling down. The duration from injury to hospital was form 20 minutes to 5 days (mean 135 min). After tibia bone traction for 5 to 8 days, the operation were performed by double-plating fixation via anteriormiddle approach, and autograft of iliac bone or allograft bone grafting were given to bone defect. Knee joint function was evaluated according to Merchanetal criteria.</p><p><b>RESULTS</b>The operation time was from 110 to 160 min, with an average of 135 min, the blood loss was from 300 ml to 500 ml,with an average of 400 ml. Post-operative wound were stage I healing. All patients were followed up from 16 to 36 months (mean 24 months). No infection, reduction loss, nonunion, deep vein thrombosis occurred. Bone healing time was for 18 to 24 weeks with an average of 21 weeks. According to the Merchanetal criteria, 4 cases got excellent results, 6 good, 1 fair and 1 poor.</p><p><b>CONCLUSION</b>Double-plating fixation via anteriormiddle approach for type C3 distal femoral fractures is an effective way, which has advantages of obvious exposure, simple manipulation, anatomical reduction, stable fixation. However,operation indications and operating instructions should be strictly followed.</p>