The inhibition effect on methicillin resistant staphylococcus aureus N31 5 of human βdefensin 3 combined with antibiotics / 预防医学

Objective To analyze the inhibition effect of antimicrobial peptide human βdefensin 3 (hBD3 )against methicillin resistant Staphylococcus aureus (MRSA)N31 5.Methods Antimicrobial peptide hBD3 was synthesized.The direct inhibition on N31 5 was detected by the MIC (minimal inhibitory concentration,MIC)test,antibacterial activity test and the analysis on some important functional genes.The peptide was combined with penicillin,tetracycline,cephradine, rifampin,vancomycin,cefuroxime and chloramphenicol to evaluate the effects on antibiotic MIC values.Results The MIC of hBD3 against N31 5 was 8 μg/mL and the bactericidal activity was observed while the hBD3 concentration reached 2 μg/mL.The peptide hBD3 could improve the expression of agr and reduce the expression of icaA.With 5 μg/mL peptide hBD3,the MIC values against N31 5 of penicillin,cephradine,vancomycin,cefuroxime and chloramphenicol were dropped down.Conclusion There is considerable bactericidal activity of antimicrobial peptide hBD3 against MRSA N31 5. Antibiotic peptide hBD3 could be used to prevent the spread of antibiotic resistant bacteria in the future.

Mitochondrial proteomic analysis of isopsoralen protection against oxidative damage in human lens epithelial cells / 中国结合医学杂志

<p><b>OBJECTIVE</b>To investigate the protective effects of the natural medicinal monomer isopsoralen (ISR) with estrogenic activity against oxidative damage in human lens epithelial cells B3 (HLE-B3) caused by hydrogen peroxide (H(2)O(2)) and to pursue the possible mitochondrial proteomic regularity of the protective effects.</p><p><b>METHODS</b>HLE-B3 cells were treated with H(2)O(2) (300 μ mol/L), β-estradiol (E(2): 10(-8) mol/L) and H(2)O(2), ISR (10(-5) mol/L) and H(2)O(2), or left untreated. Altered expressions of all mitochondrial proteins were analyzed by protein array and surfaceenhanced laser desorption ionization time of flight mass spectrometry (SELDI-TOF-MS). The mass/charge (m/z) ratios of each peak were tested by the Kruskal-Wallis rank sum test, and the protein peak value of the m/z ratio for each treatment by pair comparison was analyzed with the Nemenyi test.</p><p><b>RESULTS</b>H(2)O(2) up-regulated the expressions of two protein spots (with m/z of 6532 and 6809). E(2) mitigated the oxidative damage, and the expression of one protein spot (m/z 6532) was down-regulated. In contrast, ISR down-regulated both of protein spots (m/z 6532 and 6809).</p><p><b>CONCLUSIONS</b>ISR could effectively inhibit H(2)O(2)-induced oxidative damage in HLE-B3 cells. The protein spot at m/z of 6532 might be the target spot of ISR against oxidative damage induced by H(2)O(2).</p>

Effects of ecdysterone on the expression of NF-kappaB p65 in H2O2 induced oxidative damage of human lens epithelial cells / 中国中西医结合杂志

<p><b>OBJECTIVE</b>To study the effects of ecdysterone (ECR) on the expression of nuclear factor (NF)-kappaB in H2O2 induced oxidative damage of human lens epithelial cells (HLECs).</p><p><b>METHODS</b>The cultured HLECs were divided into 5 groups, i.e., the control group, the H2O2 group, the beta-estradiol (E2) group, the ECR group, and the pyrrolidine dithiocarbamate group (PDTC) group. The expression rate of NF-kappaB p65 in the HLECs were detected by flow cytometer (FCM).</p><p><b>RESULTS</b>The expression of NF-kappaB p65 occurred in normal HLECs (9. 53%). The expression rate of NF-kappaB p65 in the H2O2 group obviously increased (39.87%, P < 0.01). The expression rate of NF-kappaB p65 in the PDTC group obviously decreased (5.90%, P < 0.01). The expression rates of NF-kappaB p65 in the ECR group (13.99%) and the E2 group (25.18%) ranged between the control group and the H2O2 group, but still lower than that of the H2O2 group (P < 0.01).</p><p><b>CONCLUSIONS</b>The activation of NF-kappaB in the HLECs could be induced by H2O2 ECR with the estrogenic activity could effectively inhibit the activation of NF-kappaB.</p>

Magnetic resonance imaging of ovarian carcinosarcoma: correlation to the clinicopathological findings / 南方医科大学学报

<p><b>OBJECTIVE</b>To investigate magnetic resonance imaging (MRI) characteristics of ovarian carcinosarcoma and the diagnostic value of MRI.</p><p><b>METHODS</b>The MRI features of ovarian carcinosarcoma and clinical data of 5 patients with ovarian carcinosarcoma were reviewed. All the lesions were confirmed by surgery and pathological examination.</p><p><b>RESULTS</b>MRI of ovarian carcinosarcoma in the 5 cases all showed large tumor mass and heterogeneous high-intensity on T2-weighted images and low-intensity on T1-weighted images, with laminar or stripe-like enhancement. Hemorrhage and necrosis were also displayed in some lesions. In two cases, the tumors invaded the greater omentum, sigmoid colon and the body of the uterus, with regional lymph node involvement. Pelvic effusion was observed in all the cases with pelvic hematocele in 1 case.</p><p><b>CONCLUSION</b>MRI is useful in the detection and staging of ovarian carcinosarcoma.</p>