ABSTRACT
<p><b>OBJECTIVE</b>To study the effects of astragaloside on the expression of insulin-like growth factor-1 (IGF-1) and associated proteins in mice with viral myocarditis.</p><p><b>METHODS</b>Sixty-five 4-week-old BALB/C mice were randomly divided into 5 groups: normal control, astragaloside control, untreated myocarditis, low-dose and high-dose astragaloside-treated myocarditis. The BALB/C mice in the later three groups were intraperitoneally injected with CVB3. The low-dose and high-dose astragaloside-treated myocarditis groups were given astragaloside of 0.07 and 0.6 mg/kg•d, respectively by intragastric administration. Fifteen days later, the samples of blood and muscular tissues were obtained. The expression of IGF-1 in plasma was measured using ELISA. The levels of IGF-1 and associated proteins in muscular tissues were measured by immunohistochemistry. The expression of IGF-1 mRNA in muscular tissues was examined by RT-polymerase chain reaction (RT-PCR).</p><p><b>RESULTS</b>The expression of IGF-1 and associated proteins increased significantly in mice infected with CVB3. High-dose astragaloside treatment reduced the expression of IGF-1 and associated proteins, but low-dose astragaloside did not.</p><p><b>CONCLUSIONS</b>High-dose astragaloside may reduce the expression of IGF-1 and associated proteins in mice with acute viral myocarditis, possibly thus providing protective effects on muscular tissues.</p>
Subject(s)
Animals , Male , Mice , Acute Disease , Coxsackievirus Infections , Drug Therapy , Metabolism , Drugs, Chinese Herbal , Therapeutic Uses , Enterovirus B, Human , Insulin-Like Growth Factor Binding Protein 3 , Insulin-Like Growth Factor I , Genetics , Mice, Inbred BALB C , Myocarditis , Drug Therapy , Metabolism , Myocardium , Chemistry , RNA, Messenger , Receptor, IGF Type 1 , Saponins , Therapeutic Uses , Triterpenes , Therapeutic UsesABSTRACT
Objective To investigate the changes of insulin-like growth factor-1(IGF-1) and its associated protein in Balb/C mice with viral myocarditis.Methods Sixty Balb/C mice were randomly divided into 2 groups:infected group(n=40) and control group(n=20).Mice in infected group were inoculated intraperitoneally with coxsackievirus B3(CVB3) while control mice with 0.1mL of Eagle′s minimal essential medium(EMEM).Blood and myocardial musculature of all mice were collected on the day of 3,7,15 and 30 after inoculation.The expression of IGF-1 and its associated protein in plasma or myocardium were detected respectively by enzyme-labeled immunosorbent assay(ELISA),immunohistochemistry and reverse transcription polymerase chain reaction(RT-PCR).Results The expression of IGF-1 and its associated protein of mice with viral myocarditis was higher than those in control group and gradual ascent after mice being infected with virus.The expression reached summit on d15,then decreased on d30,but still sustain in a high level.The difference was significant(F=19.53 P