ABSTRACT
Objective To investigate the expression and clinical significance of plasma microRNA 10b (miRNA-10b) in patients with cervical cancer.Methods The levels of plasma miRNA-10b,SCCA and CEA were detected by RT-PCR in 168 patients with cervical cancer,68 patients with CIN (CIN group) and 45 healthy women (control group),analyzed the relation between miRNA 10b expression and clinicopathological features of cervical cancer.The sensitivity and specificity of miRNA-10b,SCCA and CEA in the diagnosis of cervical cancer were evaluated by ROC curve,and the relationship between three indexes and cervical cancer were analyzed by multivariate Logistic regression model.Correlation analysis of plasma miRNA-10b and SCCA,CEA in patients with cervical cancer was analysed by Pearson.Results The levels of miRNA-10b,SCCA and CEA in the cervical cancer group were significantly higher than those in the CIN group and the control group[miRNA-10b(2-△△Ct):5.83± 1.84 vs 2.64±0.92 and 2.38±0.75;SCCA(ng/ml):8.74±2.26 vs 1.97±0.62 and 0.61±0.15;CEA (ng/ml):5.71±2.15 vs 1.56±0.58 and 1.34±0.16,F=17.842,13.614,8.273,all P＜0.01].The level of plasma miRNA-10b expression was correlated with clinical stage,lymph node metastasis,depth of invasion and SCCA level in patients with cervical cancer(t/F=19.287,21.528,5.672,5.284,P＜0.05).Plasma miRNA-10b,SCCA and CEA and three combined diagnosis of cervical cancer of AUC (95％CI) were 0.836(0.752～0.924),0.795(0.722～0.875),0.664(0.596～0.738) and 0.882(0.794～0.958),respectively.The optimal cut-off values were 4.26,6.58 ng/ml and 4.05 ng/ml,respectively.Logistic regression analysis showed that elevated plasma miRNA-10b and SCCA levels were independent risk factors for cervical cancer[OR(95％CI)=1.816(1.629～2.483),OR(95％CI) =1.427(1.206～ 1.975)].Plasma miRNA-10b was positively correlated with SCCA in patients with cervical cancer(r=0.637,P＜0.01).Conclusion Plasma miRNA-10b will be expected to be a molecular marker for the early diagnosis of cervical cancer.The combination of SCCA and CEA can improve the diagnostic accuracy of cervical cancer.
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the protective effects of selenium on rat hippocampal neurons against ischemia-reperfusion (IR) injury.</p><p><b>METHODS</b>Thirty-two rats were randomly divided into sham-operated group, IR group and selenium-treated group, and in the latter two groups, cerebral IR injury was induced by middle cerebral artery occlusion; Na2SeO3 treatment was administer in selenium-treated group. At 14 days after reperfusion, the brain tissues were harvested from the rats and hippocampal neuron injuries were observed by TUNEL and Methylene Blue staining. The levels of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and nerve growth factor (NGF) in the hippocampal tissues were measured by ELISA.</p><p><b>RESULTS</b>Compared with IR group, the rats in selenium-treated group showed no significant increase in the expression of m-NGF (P>0.05), but pro-NGF expression was significantly increased (P<0.05) in the hippocampal tissue. Na2SeO3 treatment significantly inhibited the expressions of TNF-α and IL-1β and decreased the apoptosis of hippocampal neurons following cerebral IR injury (P<0.05).</p><p><b>CONCLUSION</b>Selenium produces antiapoptotic effect to protect the hippocampal neurons following cerebral IR injury possibly not by increasing the level of m-NGF but by decreasing the expressions of the inflammatory factors.</p>