ABSTRACT
When this paper was first published the following ethical statement was omitted in error: This study was carried out in strict accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of Tianjin Institute of Pharmaceutical Research. The authors would like to apologise for any inconvenience caused.
ABSTRACT
When this paper was first published the following ethical statement was omitted in error: This study was approved by the Ethics Committee of AAALAC (NO. 001488) and carried out in strict accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of Tianjin Institute of Pharmaceutical Research. The authors would like to apologise for any inconvenience caused. DOI of original article: https://doi.org/10.1016/j.chmed.2018.12.002
ABSTRACT
Objective: To establish a model of retinal vein occlusion (RVO) in rats and to study the effect of puerarin on ischemic retinal disease and the corresponding mechanism. Methods: RVO was induced in 10 adult male Sprague-Dawley (SD) rats by laser photothrombosis. Retinal blood flow was examined before and after 1 h of the operation, and model rats with the retinal vein blood flow decreasing by 50% compared to the basic value were chosen and then puerarins (20, 40, and 80 mg/kg) were given. The levels of vascular endothelial growth factor (VEGF), interleukin-1β (IL-1β), and nitric oxide (NO) were analyzed. In addition, the histopathology of RVO-eyes was performed. Results: RVO-eyes displayed the signs of retinal damage and ischemia on Doppler Flowmeter and histopathology. Puerarin (20, 40, and 80 mg/kg) increased blood flow by 9.3% (P < 0.05), 33.1% (P < 0.001), and 41.5% (P < 0.001), respectively. On the other hand, the histological changes were less severe at different degrees, relieving the symptoms such as edematous and thick neuroretinal layers, lax, edematous, and disorganized optic fibers layers, swollen and confused inner and outer nuclear layer. Besides, dose-dependent decrease of VEGF and IL-1β and increase of NO in vitreous fluid were observed, with respect to the model group. Conclusion: A rat model of laser photochemical-induced RVO is established and a decrease in the retinal blood flow and histological damage is detected. The puerarin has therapeutic benefit in the rat model of RVO, through the pathway of neovascularization, anti-inflammation, and increase of NO. © 2014 Tianjin Press of Chinese Herbal Medicines.
ABSTRACT
Objective: To investigate the therapeutic effect and possible mechanisms of Chinese ptent medicine Naodesheng (NDS) on repeated transient global cerebral ischemia (GCI) in mice. Methods: The repeated transient GCI mice were induced by bilateral carotid arteries ligation, and were randomly divided into model group, Sham group without arteries ligation, NDS groups (1.25 and 2.5 g/kg) and positive control (vinpocetine 3.1 mg/kg, VP) group. After oral administration once daily for successive 7 d, the transient GCI was induced. The degree of neurological deficit, histological changes, and neurons loss in the hippocampus were evaluated. In order to investigate the possible mechanisms, the oxidative stress and inflammatory factor were measured after 24 h of GCI. Comparison among multiple groups was performed with one-way analysis of variance (ANOVA). Results: NDS could significantly alleviate the neurological function impairment, histological injury, and neurons loss, increase the superoxide dismutase (SOD) activity, decrease the content of malondialdehyde (MDA), and reduce inflammatory factor in the ischemic brain tissue. Conclusion: NDS could significantly reduce brain injury induced by global ischemia, and its mechanism is closely associated with anti-oxidation and anti-inflammation.