ABSTRACT
Objective To study the temozolomide combined with curcumin on the inhibitory effect and apoptosis of the C6 glioma cells. Methods The C6 glioma cells were treated with temozomide in combination with curcumin. The anti proliferation effect of liposomes on the C6 glioma cells was investigated by using the method of sulforhodamine B (SRB). Flow cytometry was used to detect apoptosis of the C6 glioma cells. Confocal laser scan-ning microscope was used to observe apoptosis and location in the C6 glioma cells. Results The results of SRB as-say showed that temozolomide in combination with curcumin inhibition rate were (91.22 ± 0.51)%in 48 h of the C6 glioma cells; Flow cytometry showed that the apoptosis rate were (33.15 ± 0.79)% with temozolomide (5 μmol/L) in combination with curcumin (10 μmol/L). Laser scanning confocal scanning microscope indicated that the apop-tosis of in the C6 glioma cells treated with temozolomide in combination with curcumin was more than that of free drug. Conclusion The temozolomide in combination of curcumin can inhibit the growth and induce apoptosis of the C6 glioma cells.
ABSTRACT
[Objective] To determine dormancy mechanism of Taxus mairei(Lemee et Levl.) S.Y.Hu ex Liu Seeds,the best method of dormancy breaking.[Method]The seeds were handled by lotic water or excitatory autacoid or cold-warm-cold stratification treatment or cold-warm-cold stratification treatment with excitatory autacoid,then compare withgermination inhibition of Brassica campestris seeds handling with water solution from the seeds after four dormancy breaking methods,to dertemine the best dormancy breaking method.[Conclusion] The best damancy breaking method was cold(4℃)—warm(23℃)—cold(4℃) stratification treatment with 0.05% gibberellic acid for 120 days.