ABSTRACT
Spore coat proteins, such as CotB, CotC, CotG et al, are able to efficiently display exogenous protein on spore surface for preparing oral vaccines or enzymes. CotX is another structural protein of spore coats of Bacillus subtilis. To investigate whether CotX could carry target protein onto the spore surface, we constructed a recombinant integrative plasmid, designated as pJS749, which carries a recombinant cotX-gfp gene under the control of the cotX promoter. We transformed pJS749 into Bacillus subtilis 168(trp-), an alpha-amylase inactivated mutant DRJS749 was selected and confirmed to be a double crossover integrant, where cotX-gfp fragment was integrated into the chromosome. After induction of spore formation, significant green fluorescence was observed on spore surface of strain DRJS749 under fluorescent microcopy. This suggests that CotX is associated with the outer part of the coat. CotX can therefore be used as a molecular vehicle for spore surface display of exogenous proteins.
Subject(s)
Bacillus subtilis , Genetics , Metabolism , Physiology , Bacterial Proteins , Genetics , Gene Expression Regulation, Bacterial , Green Fluorescent Proteins , Genetics , Recombinant Fusion Proteins , Genetics , Spores, Bacterial , Genetics , MetabolismABSTRACT
BOD,the same for the water quality factor and zooplankton.The water quality index DO,COD and TN were the key factors to influence phytoplankton density.Conclusion The diversity of phytoplankton and zooplankton in Neijiang River,Zhenjiang is considered as better.
ABSTRACT
Objective To isolate and identify the pathogenic bacterial strain Myroides odoratus from municipal sewage in Zhenjiang city.Methods With selective medium,a pathogenic bacterial strain JD23 was isolated from the city sewage.Combining the modern molecular biology technology methods and bioinformatics methods,its taxonomic status was confirmed by 16S rDNA gene sequence homologous analysis and phylogenetic tree.The traditional experiments of physiology,biochemistry and morphology were also studied.Results Strain JD23 exhibited the highest levels(98%) of similarity to the Myroides odoratus M58777.2.Combining all the results of several methods and morphological observation,strain JD23 was finally identified as the bacterial classification Myroides odoratus.Conclusion There was a pathogenic microorganism,Myroides odoratus,in municipal sewage of Zhenjiang city.