Shear bond strength of dentin bonding agents cured with a Plasma Arc curing light / 대한치과보존학회지

The objective of this study was to compare dentin shear bond strength (DSBS) of dentin bonding agents (DBAs) cured with a plasma arc (PAC) light curing unit (LCU) and those cured with a light emitting diode (LED) LCU. Optical properties were also analyzed for Elipar freelight 2 (3M ESPE); LED LCU, Apollo 95E (DMT Systems); PAC LCU and VIP Junior (Bisco); Halogen LCU. The DBAs used for DSBS test were Scotchbond Multipurpose (3M ESPE), Singlebond 2 (3M ESPE) and Clearfil SE Bond (Kuraray). After DSBS testing, fractured specimens were analyzed for failure modes with SEM. The total irradiance and irradiance between 450 nm and 490 nm of the LCUs were different. LED LCU showed narrow spectral distribution around its peak at 462 nm whereas PAC and Halogen LCU showed a broad spectrum. There were no significant differences in mean shear bond strength among different LCUs (P > 0.05) but were significant differences among different DBAs (P < 0.001).

Prospective clinical evaluation of three different bonding systems in class V resin restorations with or without mechanical retention / 대한치과보존학회지

The purpose of this study is to evaluate prospectively the effect of different bonding systems and retention grooves on the clinical performance of resin restorations in non-carious cervical lesions (NCCLs). Thirty-nine healthy adults who had at least 2 NCCLs in their premolar areas were included in this study. One hundred and fifty teeth were equally assigned to six groups: (A) Scotchbond Multi-Purpose (SBMP, 3M ESPE, St. Paul, MN, USA, 4th generation bonding system) without retention grooves; (B) SBMP with retention grooves; (C) BC Plus (Vericom Co., Anyang, Gyeonggido, Korea, 5th generation bonding system) without retention grooves; (D) BC Plus with retention grooves; (E) Adper Prompt (3M ESPE, Seefeld, Germany, 6th generation bonding system) without retention grooves; (F) Adper Prompt with retention grooves. All cavities were filled with a hybrid composite resin, Denfil (Vericom Co., Anyang, Gyeonggido, Korea) by one operator. Restorations were evaluated at baseline and at 6-month recall, according to the modified USPHS (United States Public Health Service) criteria. Additionally, clinical photographs were taken and epoxy resin replicas were made for SEM evaluation. At 6-month recall, there were some differences in the number of alpha ratings among the experimental groups. But, despite the differences in the number of alpha ratings, there was no significant difference among the 3 adhesive systems (p > 0.05). There was also no significant difference between the groups with or without mechanical retention (p > 0.05). Follow-ups for longer periods than 6 months are needed to verify the clinical performance of different bonding systems and retention grooves.

Effect of additional coating of bonding resin on the microtensile bond strength of self-etching adhesives to dentin / 대한치과보존학회지

This study investigated the hypothesis that the dentin bond strength of self-etching adhesive (SEA) might be improved by applying additional layer of bonding resin that might alleviate the pH difference between the SEA and the restorative composite resin. Two SEAs were used in this study; Experimental SEA (Exp, pH: 1.96) and Adper Prompt (AP, 3M ESPE, USA, pH: 1.0). In the control groups, they were applied with two sequential coats. In the experimental groups, after applying the first coat of assigned SEAs, the D/E bonding resin of All-Bond 2 (Bisco Inc., USA, pH: 6.9) was applied as the intermediate adhesive. Z-250 (3M ESPE, USA) composite resin was built-up in order to prepare hourglass-shaped specimens. The microtensile bond strength (MTBS) was measured and the effect of the intermediate layer on the bond strength was analyzed for each SEA using t-test. The fracture mode of each specimen was inspected using stereomicroscope and Field Emission Scanning Electron Microscope (FE-SEM). When D/E bonding resin was applied as the second coat, MTBS was significantly higher than that of the control groups. The incidence of the failure between the adhesive and the composite or between the adhesive and dentin decreased and that of the failure within the adhesive layer increased. According to the results, applying the bonding resin of neutral pH can increase the bond strength of SEAs by alleviating the difference in acidity between the SEA and restorative composite resin.

Rheological characterization of composites using a vertical oscillation rheometer / 대한치과보존학회지

OBJECTIVE: The purpose of this study was to investigate the viscoelastic properties related to handling characteristics of composite resins. METHODS: A custom designed vertical oscillation rheometer (VOR) was used for rheological measurements of composites. The VOR consists of three parts: (1) a measuring unit, (2) a deformation induction unit and (3) a force detecting unit. Two medium viscous composites, Z100 and Z250 and two packable composites, P60 and SureFil were tested. The viscoelastic material function, including complex modulus E* and phase angle delta, were measured. A dynamic oscillatory test was used to evaluate the storage modulus (E'), loss modulus (E") and loss tangent (tandelta) of the composites as a function of frequency (omega) from 0.1 to 20 Hz at 23degrees C. RESULTS: The E' and E" increased with increasing frequency and showed differences in magnitude between brands. The E*s of composites at omega = 2 Hz, normalized to that of Z100, were 2.16 (Z250), 4.80 (P60) and 25.21 (SureFil). The magnitudes and patterns of the change of tandelta of composites with increasing frequency were significantly different between brands. The relationships between the complex modulus E*, the phase angle delta and the frequency omega were represented by frequency domain phasor form, E* (omega) = E*e(idelta) = E* 45 degree angle delta. CONCLUSIONS: The viscoelasticity of composites that influences handling characteristics is significant different between brands. The VOR is a relatively simple device for dynamic, mechanical analysis of high viscous dental composites. The locus of frequency domain phasor plots in a complex plane is a valuable method of representing the viscoelastic properties of composites.

Dentin bond strength of bonding agents cured with light emitting diode / 대한치과보존학회지

This study compared the dentin shear bond strengths of currently used dentin bonding agents that were irradiated with an LED (Elipar FreeLight, 3M-ESPE) and a halogen light (VIP, BISCO). The optical characteristics of two light curing units were evaluated. Extracted human third molars were prepared to expose the occlusal dentin and the bonding procedures were performed under the irradiation with each light curing unit. The dentin bonding agents used in this study were Scotchbond Multipurpose (3M ESPE), Single Bond (3M ESPE), One-Step (Bisco), Clearfil SE bond (Kuraray), and Adper Prompt (3M ESPE). The shear test was performed by employing the design of a chisel-on-iris supported with a Teflon wall. The fractured dentin surface was observed with SEM to determine the failure mode. The spectral appearance of the LED light curing unit was different from that of the halogen light curing unit in terms of maximum peak and distribution. The LED LCU (maximum peak in 465 nm) shows a narrower spectral distribution than the halogen LCU (maximum peak in 487 nm). With the exception of the Clearfil SE bond (P 0.05). The results can be explained by the strong correlation between the absorption spectrum of camphoroquinone and the narrow emission spectrum of LED.

Rheological properties of resin composites according to the change of monomer and filler compositions / 대한치과보존학회지

OBJECTIVES: The aim of this study was to investigate the effect of monomer and filler compositions on the rheological properties related to the handling characteristics of resin composites. METHODS: Resin matrices that Bis-GMA as base monomer was blended with TEGDMA as diluent at various ratio were mixed with the Barium glass (0.7 um and 1.0 um), 0.04 um fumed silica and 0.5 um round silica. All used fillers were silane treated. In order to vary the viscosity of experimental composites, the type and content of incorporated fillers were changed. Using a rheometer, a steady shear test and a dynamic oscillatory shear test were used to evaluate the viscosity (eta) of resin matrix, and the storage shear modulus (G'), the loss shear modulus (G"), the loss tangent (tandelta) and the complex viscosity (eta*) of the composites as a function of frequency omega = 0.1-100 rad/s. To investigate the effect of temperature on the viscosity of composites, a temperature sweep test was also undertaken. RESULTS: Resin matrices were Newtonian fluid regardless of diluent concentration and all experimental composites exhibited pseudoplastic behavior with increasing shear rate. The viscosity of composites was exponentially increased with increasing filler volume%. In the same filler volume, the smaller the fillers were used, the higher the viscosities were. The effect of filler size on the viscosity was increased with increasing filler content. Increasing filler content reduced tandelta by increasing the G' further than the G". The viscosity of composites was decreased exponentially with increasing temperature.

Influence of cavity size and restoration methods on the cusp deflection in composite restoration / 대한치과보존학회지

The aim of this study was to measure the cusp deflection during composite restoration for MOD cavity in premolar and to examine the influence of cavity dimension, C-factor and restoration method on the cusp deflection. Thirty extracted maxillary premolar were prepared to four different sizes of MOD cavity and divided into six groups. The width and depth of the cavity were as follows. Group 1; 1.5 x 1 mm, Group 2; 1.5 x 2 mm, Group 3; 3 x 1 mm, and Group 4-6; 3 x 2 mm respectively. Group 1-4 were restored using bulk filling method with Z-250 composite. However, Group 5 was restored incrementally, and Group 6 was restored with an indirect resin inlay. The cusp deflection was recorded at the buccal and lingual cusp tips using LVDT probe for 10,000 seconds. The measured cusp deflections were compared between groups, and the relationship between the cube of the length of cavity wall/the cube of the thickness of cavity wall (L3 / T3), C-factor and cusp deflection or %flexure (100 x cuspal deflection / cavity width) was analyzed. The cusp deflection of Group 1-4 were 12.1 microm, 17.2 microm, 16.2 microm and 26.4 microm respectively. The C-factor was related to the %flexure rather than the cusp deflection. There was a strong positive correlationship between the L3 / T3 and the cusp deflection. The cusp deflection of Group 5 and 6 were 17.4 microm and 17.9 microm respectively, which are much lower value than that of Group 4.

The effect of cavity wall property on the shear bond strength test using iris method / 대한치과보존학회지

OBJECTIVES: In the unique metal iris method, the developing interfacial gap at the cavity floor resulting from the cavity wall property during polymerizing composite resin might affect the nominal shear bond strength values. The aim of this study is to evaluate that the iris method reduces the cohesive failure in the substrates and the cavity wall property effects on the shear bond strength tests using iris method. MATERIALS AND METHODS: The occlusal dentin of 64 extracted human molars were randomly divided into 4 groups to simulate two different levels of cavity wall property (metal and dentin iris) and two different materials (ONE-STEP(R) and ALL-BOND(R) 2) for each wall property. After positioning the iris on the dentin surface, composite resin was packed and light-cured. After 24 hours the shear bond strength was measured at a crosshead speed of 0.5 mm/min. Fracture analysis was performed using a microscope and SEM. The data was analyzed statistically by a two-way ANOVA and t-test. RESULTS: The shear bond strength with metal iris was significant higher than those with dentin iris (p = 0.034). Using ONE-STEP(R), the shear bond strength with metal iris was significant higher than those with dentin iris (p = 0.005), but not in ALL-BOND(R) 2 (p = 0.774). The incidence of cohesive failure was very lower than other shear bond strength tests that did not use iris method. CONCLUSIONS: The iris method may significantly reduce the cohesive failures in the substrates. According to the bonding agent systems, the shear bond strength was affected by the cavity wall property.

MMP-1 and TIMP-1 production in MG-63 cells stimulated with Prevotella nigrescens lipopolysaccharide / 대한치과보존학회지

The purpose of this study is to monitor the secretion of matrix metalloproteinase-1 (MMP-1) and tissue inhibitor of metalloproteinase-1 (TIMP-1) produced by human osteosarcoma cell line (MG63) stimulated with Prevotella nigrescens lipopolysaccharides (LPS), and to compare the level of secretion before and after the treatment of calcium hydroxide on P. nigrescens LPS. LPS was extracted and purified from anaerobically cultured P. nigrescens. MG63 cells were stimulated by the LPS (0, 1, 10 microg/ml) or LPS (10 microg/ml) pretreated with 12.5 mg/ml of Ca(OH)2 for 3 days. Total RNA was isolated from the cell, and real-time quantitative polymerase chain reaction (PCR) was performed for quantification of MMP-1 and TIMP-1. The results were as follows. 1. MMP-1 mRNA expression at 48 hr was highly increased by stimulation with P. nigrescens LPS. The increase was dose-dependent. 2. When stimulated with 1 microg/ml of LPS, TIMP-1 mRNA expression was highly increased at 24 hr and 48 hr. However, TIMP-1 expression was suppressed at higher concentration (10 microg/ml). 3. When P. nigrescens LPS was pretreated with Ca(OH)2, MMP-1 and TIMP-1 gene expression was downregulated. The results of this study suggest that transcriptional regulation of MMP-1 and TIMP-1 by P. nigrescens LPS could be one of the important mechanisms in bone resorption of periapical inflammation. The result of calcium hydroxide on MMP-1 and TIMP-1 gene expression suppression shows that calcium hydroxide detoxified bacterial LPS and thus should be used the medication of choice for intracanal dressings in root canal infected with black-pigmented bacteria.

Influence of the curing time for the adhesive on the oxygen-inhibited layer thickness and the shear bond strength to dentin / 대한치과보존학회지

OBJECTIVES: This study investigated the hypothesis that increasing light-curing time would leave the oxygen-inhibited layer (OIL) of the adhesive thinner, and in turn, result in lower shear bond strength (SBS) than those obtained by the routine curing procedures. METHODS: 120 human extracted posterior teeth were randomly divided into three groups for bonding with three adhesives: All Bond 2(R), One Step(R), and Adper Prompt(R). They were subsequently divided into four subgourps with different light-curing time (10, 20, 30 and 60 s). The assigned adhesives were applied on superficial occlusal dentin according to the manufacturer's instructions and cured with one of the four curing times. Composite resin cylinder, 2.35 mm in diameter, were built on the cured adhesive and light-cured for 40 s. SBS were measured after 24 h from the bonding using a universal testing machine (crosshead speed 1.0 mm/min). The relative thickness of the OIL and the degree of conversion (DC) were determined from the adhesive on a slide glass using FT-NIR in an absorbance mode. Data were analysed with One-way ANOVA and Duncan's multiple test (p 0.05), those of All Bond 2 decreased significantly (p 0.05). Although the DC of All-Bond 2 were statistically not different with increasing cure time (p > 0.05), those of One-Step and Adper Prompt showed an increasing trends with increasing cure time (p < 0.05). CONCLUSIONS: Increasing light-curing time did not affect on the relative thickness of the OIL of the adhesives, and in turn, on the SBS to dentin.

Influence of the curing time for the adhesive on the oxygen-inhibited layer thickness and the shear bond strength to dentin / 대한치과보존학회지

OBJECTIVES: This study investigated the hypothesis that increasing light-curing time would leave the oxygen-inhibited layer (OIL) of the adhesive thinner, and in turn, result in lower shear bond strength (SBS) than those obtained by the routine curing procedures. METHODS: 120 human extracted posterior teeth were randomly divided into three groups for bonding with three adhesives: All Bond 2(R), One Step(R), and Adper Prompt(R). They were subsequently divided into four subgourps with different light-curing time (10, 20, 30 and 60 s). The assigned adhesives were applied on superficial occlusal dentin according to the manufacturer's instructions and cured with one of the four curing times. Composite resin cylinder, 2.35 mm in diameter, were built on the cured adhesive and light-cured for 40 s. SBS were measured after 24 h from the bonding using a universal testing machine (crosshead speed 1.0 mm/min). The relative thickness of the OIL and the degree of conversion (DC) were determined from the adhesive on a slide glass using FT-NIR in an absorbance mode. Data were analysed with One-way ANOVA and Duncan's multiple test (p 0.05), those of All Bond 2 decreased significantly (p 0.05). Although the DC of All-Bond 2 were statistically not different with increasing cure time (p > 0.05), those of One-Step and Adper Prompt showed an increasing trends with increasing cure time (p < 0.05). CONCLUSIONS: Increasing light-curing time did not affect on the relative thickness of the OIL of the adhesives, and in turn, on the SBS to dentin.

The effect of viscosity, specimen geometry and adhesion on the linear polymerization shrinkage measurement of light cured composites / 대한치과보존학회지

OBJECTIVES: The aim of study was to investigate the effect of flow, specimen geometry and adhesion on the measurement of linear polymerization shrinkage of light cured composite resins using linear shrinkage measuring device. METHODS: Four commercially available composites - an anterior posterior hybrid composite Z100, a posterior packable composite P60 and two flowable composites, Filtek flow and Tetric flow - were studied. The linear polymerization shrinkage of composites was determined using 'bonded disc method' and 'non-bonded' free shrinkage method at varying C-factor in the range of 1~8 by changing specimen geometry. These measured linear shrinkage values were compared with free volumetric shrinkage values. The viscosity and flow of composites were determined and compared by measuring the dropping speed of metal rod under constant load. RESULTS: In non-bonded method, the linear shrinkage approximated one third of true volumetric shrinkage by isotropic contraction. However, in bonded disc method, as the bonded surface increased the linear shrinkage increased up to volumetric shrinkage value by anisotropic contraction. The linear shrinkage value increased with increasing C-factor and approximated true volumetric shrinkage and reached plateau at about C-factor 5~6. The more flow the composite was, reduced linear shrinkage was measured by compensation radial flow.

Effects of filler addition to bonding agents on shear bond strength / 대한치과보존학회지

No abstract available.

Microleakage of microfill and flowable composite resins in class V cavity after load cycling / 대한치과보존학회지

Low-viscosity composite resins may produce better sealed margins than stiffer compositions (Kemp-Scholte and Davidson, 1988; Crim, 1989). Flowable composites have been recommended for use in Class V cavities but it is also controversial because of its high rates of shrinkage. On the other hand, in the study comparing elastic moduli and leakage, the microfill had the least leakage (Rundle et al. 1997). Furthermore, in the 1996 survey of the Reality Editorial Team, microfills were the clear choice for abfraction lesions. The purpose of this study was to evaluate the microleakage of 6 compostite resins (2 hybrids, 2 microfills, and 2 flowable composites) with and without load cycling. Notch-shaped Class V cavities were prepared on buccal surface of 180 extracted human upper premolars on cementum margin. The teeth were randomly divided into non-load cycling group (group 1) and load cycling group (group 2) of 90 teeth each. The experimental teeth of each group were randomly divided into 6 subgroups of 15 samples. All preparations were etched, and Single bond was applied. Preparations were restored with the following materials (n=15): hybrid composite resin [Z250(3M Dental Products Inc. St.Paul, USA), Denfil(Vericom, Ahnyang, Korea)], microfill [Heliomolar RO(Vivadent, Schaan, Liechtenstein), Micronew(Bisco Inc. Schaumburg, IL, USA)], and flowable composite [AeliteFlo(Bisco Inc. Schaumburg, IL, USA), Revolution(Kerr Corp. Orange, CA, USA)]. Teeth of group 2 were subjected to occlusal load (100N for 50,000 cycles) using chewing simulator(MTS 858 Mini Bionix II system, MTS Systems Corp., Minn. USA). All samples were coated with nail polish 1mm short of the restoration, placed in 2% methylene blue for 24 hours, and sectioned with a diamond wheel. Enamel and dentin/cementum margins were analyzed for microleakage on a sclale of 0 (no leakage) to 3 (3/3 of wall). Results were statistically analyzed by Kruscal-Wallis One way analysis, Mann-Whitney U-test, and Student-Newmann-Keuls method. (p=0.05) RESULTS: 1. There was significantly less microleage in enamel margins than dentinal margins of all groups. (pA=H=M>D>Z. But there was no significant differences. 6. In enamel margins, load cycling did not affect the marginal microleakage in significant degree. 7. In dentin margins, load cycling did affect the marginal microleakage only in Revolution. (p<0.05)