ABSTRACT
Objective To explore the surgical procedure for combined heart and kidney transplantation in rats. Methods Inbred Lewis rats were used as donors and recipients. The left kidney and the heart were removed from the donors,the donor kidney was therefore transplanted to the left side. Revascularization of renal artery was constructed end-in-end by modified sleeve anastomosis. Renal veins were anastomosed using stenting technique. Ureters were anastomosed using end-to-end interrupted suture technique. The aortic and pulmonary arteries were anastomosed to the recipient abdominal aorta and inferior vena cavaLewis rats receiving right nephrectomy were assigned to a control group. Results The time of warm ischemia was 10s,and the donor nephrectomy time was (30 ± 3.0)min;the removal of donor heart was(6 ± 1.8)min,the renal cold ischemia time was(40 ± 3.2)min and heart was(70 ± 4.1)min,the meantime for recipients operation was(95 ± 5.4)min,and the graft survival rate was 80.9%. Renal function and heart rate did not differ significantly between the two groups 30 days after the procedure. Conclusions The new model for simultaneous heart and kidney transplantation in rats is feasible and reliable. This technique can be applied to basic research on multiple organ transplantation immunity.
ABSTRACT
Objective To improve arterial anastomosis method for renal transplantation model in rats.Methods Male Fisher and Lewis rats were used as kidney donors and recipients respectively,and left kidneys were harvested in situ.Revascularizations of renal artery were fashioned end-to-end by the modified sleeve anastomosis.The renal artery was placed in the orthotopic position and sutures were inserted in order to place the feeding vessel into the receiving vessel.One invaginating suture was placed starting outside the donor's renal artery wall,approximately 2 mm from the free edge,then passing through the free edge of the recipient renal artery,and lastly passing again through the donor's renal artery wall out alongside the point of entry.The sutures Were tied so that the recipient renal artery was drawn inside the donor's renal artery.Thereafter,one external stitch was placed opposite to the invaginating suture passing through the overlapped free distal edge and the adventitia of the recipient renal artery,and the suture the opposite side using the same technique.The renal veins and ureters were anastomosed using end-to-end interrupted suture technique.Results Twenty cases of rat renal transplantation were performed.The transplantation procedures took totally between 70-90 min.The time for arterial anastomosis was approximately(4.6 ± 0.6)mint the mean time for anastomosis of the renal vein in 20 grafts was(11.8 ± 1,2)min.and ureter was(12.2 ± 1.4)mia The successful rate of the model was 95 % at the 5th day.Conclusion New end-to-end technique which incorporatesa modification of the sleeve anastomosis is the safest way to perform a revascularization of renal artery.This suggests that the technique is feasible and reliable.