Clinical effect of orthodontic-repair combined therapy for maxillary anterior teeth dentition defect with malocclusion / 中国基层医药

Objective@#To investigate the clinical effect of orthodontic-repair combined therapy in the treatment of maxillary anterior teeth dentition defect and malocclusion.@*Methods@#The clinical data of 68 patients with maxillary anterior dentition defect and malocclusion were analyzed retrospectively.They were divided into control group (repair treatment) and study group (orthodontic-repair treatment) according to the different methods of treatment, 34 cases in each group.The tooth occlusal relationship, the occlusion of the anterior teeth and the arrangement of the teeth, clinical treatment effect and the treatment cost were analyzed.The satisfaction of the patients after treatment was investigated by questionnaire.The debris index and attachment loss index were detected.@*Results@#The good rate of tooth occlusal relationship, good rate of anterior teeth coverage and teeth order rate in the study group were 82.35%(28/34), 88.24%(30/34), 94.12%(32/34), respectively, which were significantly higher than those in the control group[58.82%(20/34), 67.65%(23/34), 73.53%(25/34)](χ2=4.53, 4.19, 4.53, P=0.03, 0.04, 0.03). The total effective rate of the study group was 94.12%(32/34), which was significantly higher than that of the control group[70.59%(24/34)](χ2=6.50, P=0.03). The average treatment cost of the study group was (8 124.63±386.58)yuan, which was significantly higher than (2 179.86±276.82)yuan of the control group (t=72.90, P=0.00). The patients' satisfaction of the study group was was 88.24%(30/34), which was significantly higher than that of the control group[52.94%(18/34)](χ2=10.20, P=0.00). The debris index and attachment loss index in the study group were (0.14±0.07), (5.06±1.38), respectively, which were significantly lower than those in the control group[(0.63±0.18), (6.52±1.58)](t=14.79, 14.79, P=0.00, 0.00).@*Conclusion@#Orthodontics combined with orthodontic treatment can help to improve the patients' tooth deformity, and the effect is remarkable, which can win the patients' praise, but the cost of the method is high, so patients should be considered in the selection.

Value of endobronchial ultrasound-guided localization with combination of ultrathin bronchoscope and methylene blue for small pulmonary nodules before video-assisted thoracoscopic surgery / 中华胸心血管外科杂志

Objective To analyze the value of endobronchial ultrasound-guided ultrathin bronchoscope(EUGUB) and methylene blue positioning video-assisted thoracoscopic surgery(VATS) for small pulmonary nodules.Methods Sixty patients undergoing VATS, using pre-operative EUGUB combination with methylene blue was performed .The positioning accuracy, the complications and pathological classification were analyzed .Results EUGUB combination with methylene blue positioning ac-curacy was 91.7%, meanwhile, 2 patients with hemoptysis with no conservative treatment.The postoperative histological pa-thology results showed that benign lesions and malignant lesions accounted for 28.3% and 71.7%, respectively.Conclusion The method of EUGUB combination with methylene blue in positioning of the small pulmonary nodules increases the achieve-ment ratio of VATS with few complications, and may be widely used in treatment of small pulmonary nodules with VATS.

Comparison and development of different scalp needling schools / 中国针灸

The literature and relevant books on the scalp needling schools were retrieved to collect the main scalp needling schools and compare the point location principles and needling techniques so as to optimize the scalp needling scheme. Currently there are ten major schools of scalp acupuncture, representing for example by,and, etc. The principles of stimulating localization are different among the schools, in which,'s skull line localization is mainly for the treatment of cerebrovascular diseases,prefers the penetrating technique for the treatment of pain and mental disorders according to traditional meridian theory, and the other schools take the cortical functions into account for the stimulating localization. Regarding needling techniques,stresses on the deep puncture to the periosteum, and the rest school masters puncture beneath the bonnet aponeurosis. The quick rotation of needle is required in's scalp needling,'s skull needle,and the needling technique for opening brain and promotion collateral. The long-term needle retaining is required in's scalp needling and's scalp needling. It is believed that the stimulating localization principles are not standardized, the theoretic evidences are not clear and the needling depth is different among the schools. There are no standard criteria on needling angle, depth, direction, rotating speed and needle retaining time. The evidence-based medicine is recommended. Through strict scientific design, based on the clinical and experimental evidences, it is required to determine the theoretic scientific evidence of scalp acupuncture, the specificity of stimulating areas, the effective stimulating areas and quantify the needling manipulations and clarify the optimal scheme of scalp needling.

Peripheral blood T lymphocytes cell level of different grades of cerebral gliomas patients before and after operation and its clinical significance / 重庆医学

Objective To explore the peripheral blood T lymphocytes cell level in different grades of cerebral gliomas pa-tients at the time of before and after operation and its clinical significance .Methods A total of 80 cases of brain tumor patients from February 2010 to February 2012 in this hospital were chosed as study objects ,included 57 cases of glioma ,23 cases of other brain tumors ;57 cases of glioma were divided into 19 cases of low grade group and 38 cases of high grade group accorded to WHO grading standards ,and 50 cases of healthy people in same period were selected as control group .Venous blood in three groups were extracted at 1st day and 1st week after operation ,to detected the level of T lymphocyte subsets in peripheral blood and analyze the relationship between the different grade gliomas and prognosis .Results The peripheral blood CD3 + ,CD4 + ,CD4 + /CD8 + in control group were significantly higher than that of other brain tumor group and the glioma group ,CD8 + was significantly lower than that of the two groups(P 1 group and 17 months in CD4 + /CD8 + < 1 group ,the singnificance was found in two groups(P< 0 .05) .Conclusion The immune function of patients with brain glioma is inhibited ,the higher the malignancy of the tumor ,the more obvious of im-mune inhibition ;T lymphocyte subsets level could be used as evaluating index of malignant degree and prognosis of brain glioma .

Comparison of three mouse models infected with influenza virus by aerosol / 中国实验动物学报

Objective To compare and analyze the differences and characteristics of three strain mouse models in-fected by influenza virus aerosol inhalation, and provide the reference for choosing the appropriate infection model in the re-search of pathogenesis of influenza and the development of vaccines and drugs.Method C57BL/6, BALB/c and ICR mice were infected with A/Puerto Rico/8/34 (H1N1) virus strain by aerosol inhalation.The symptoms and body weight of mice were observed every day.At 3, 7, 14 days after infection, the mice were sacrificed.The lungs of mice were weighed, then virus assay and pathological observation were carried out.Results The three strains of mice were infected.The sur-vival rate in the C57BL/6 mice was lower than those in the BALB/c and ICR mice.The lung index and viral load of C57BL/6 mice were significantly higher than those of ICR mice ( P<0.05) at 3 days after infection.The pathological changes of C57BL/6 mice were also more obvious than other two strains.Compared with other two mouse strains, the weight recovery of BALB/c mice was the slowest.The survival rate in BALB/c mice was higher than that of C57BL/6 mice and lower than that of ICR mice.The lung index and viral load were not significantly different among the three strains of in-fected mice.The pathological changes among the three strains of infected mice were similar, but the degrees of pathological changes in the BALB/c mice were milder than in the C57BL/6 mice and worse than in the ICR mice.Compared with other two mouse strains, the process of disease is similar, but the body weight, mortality, lung index, viral load, and the micro-scopic pathological changes were lighter in the ICR mice than in the other two strain mice.Conclusions The three strain mouse models can be established by influenza virus aerosol inhalation, but showing different characteristics.Appropriate strain mice can be chosen to build model according to different research purpose in the experiment.

Rare parotid gland tumors: enhanced computed tomography and pathological correlation / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To investigate the correlation between enhanced computed tomography (CT) findings and pathological results of rare parotid gland tumors, and improve diagnosis accuracy.</p><p><b>METHODS</b>The enhanced CT manifestations of 22 cases with pathologically documented rare parotid gland tumors, which included 6 cases of basal cell tumor, 5 cases of myoepithelioma, 4 cases of vascular invasion, 3 cases of lymphatic cyst, 3 cases of lipoma, and 1 case of chondrosarcoma, were retrospectively analyzed. The location, size, shape, density, and relationship with surrounding structure were evaluated on CT images.</p><p><b>RESULTS</b>The enhanced CT showed that basal cell tumors occurred in the superficial lobe of the parotid gland, with clear boundary, within the cystic lesion. The lesions were moderate to obviously enhanced, which may be accompanied by enlarged lymph nodes. Myoepithelial tumors were located in the superficial lobe of the parotid gland, with a small cystic prone and microcalcification within a few cases. The lesions were moderate to obviously enhanced. Hemangiomas of soft tissue mass prominent in the parotid gland surface were mild to significantly enhanced. Larger lesions may occupy the entire parotid gland, with uneven density and visible vein stone. The CT density values of the lymphatic cyst were usually higher. Chondrosarcoma mainly manifested cystic mass at the calcification edge. Lipoma with fat density mass exhibited clear boundary without enhancement. Fiber separation could be observed in the lesion.</p><p><b>CONCLUSION</b>CT can reflect the pathological features of rare parotid gland tumors by demonstrating their corresponding imaging features. Enhanced CT is the most effective means of imaging to identify the nature of rare tumor of the parotid gland lesions.</p>

Parotid adenolymphoma: the enhanced MSCT manifestations and clinical pathological analysis / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To investigate the imaging manifestations of 16-slice enhanced CT of parotid adenolymphoma in the parotid gland and the corresponding pathology,in order to improve the understanding of the CT imaging manifestations of parotid adenolymphoma in the parotid gland.@*METHOD@#The enhanced CT characteristics of 34 cases of parotid adenolymphoma in the parotid gland confirmed by histological pathology were retrospectively analyzed.@*RESULT@#There were totally 86 lesions in 34 cases, of which 12 cases with lesions in bilateral sides and 22 cases with lesions in unilateral side. Sixty-six lesions located behind and below the superficial lobe of the parotid gland. The lesions showed moderate to obvious enhancement at arterial phase, and the cystic region within the lesions showed no enhancement.@*CONCLUSION@#The relatively specific enhanced MSCT manifestations of parotid adenolymphoma in parotid gland include lesions located behind and below the superficial lobe of parotid gland unilaterally or bilaterally, sometimes exhibited as multiple masses, with clear edge, obvious enhancement and cystic degeneration inside.

The significance of the soluble Apo-1/Fas in serum and pleural effusion in differentiating tuberculous and malignant pleural effusion / 中国基层医药

Objective To investigate the significance of the soluble Apo-1/Fas(sApo-1/Fas) in differentiating tuberculous and malignant pleural effusion.Methods The level of sApo-1/Fas was detected by enzyme linked immunosorbent assay(ELISA).in serum and pleural effusion of 50 cases with malignant pleural effusion and 48 cases with tuberculous pleural effusion.Results The level of sApo-1/Fas in the serum and pleural effusion of malignant pleural effusion patients were ( 16.3 ± 2.5 ) μg/L and ( 38.6 ± 13.5) μg/L,significantly higher than those of tuberculous patients with pleural effusion( all P ＜ 0.05 ).The difference of rates of serum and pleural effusion in malignant pleural effusion and tuberculous pleural effusion patients was significant( P ＜ 0.05 ).The sensitivity,specificity and accuracy of sApo-1/Fas in serum and malignant pleural effusion were 66.0％ and 93.8％ and 79.6％.Conclusion The detecting of sApo-1/Fas may play some role in differentiating tuberculous and malignant pleural effusion.

The levels and clinical significance of osteopontin in serum of patients with lung cancer / 中国医师杂志

ObjectiveTo explore the levels and clinical significance of osteopontin (OPN) in serum of patients with non-small cell lung cancer (NSCLC).MethodsOPN levels of 102 cases of NSCLC,42 cases of benign lung disease and 30 cases of healthy control were measured by enzyme-linked immunosorbent assay(ELISA).ResultsThe serum OPN levels of NSCLC were significantly higher than those of benign lung disease group and healthy control group (P＜0.01).The levels of OPN in serum were compared between different gender,age,histological differentiation and pathological type groups,but there were no significant difference (P＞0.05).The serum OPN levels of Ⅲ - Ⅳ stage NSCLC patients,with lymph node metastasis,before operation patients,recrudescent patients,survival below 3 years patients were relatively higher than that in the patients with Ⅰ - Ⅱ stage,without lymph node metastasis,after operation,no recrudescent,survival over 3 years patients,and the differences were significant (P＜0.01).ConclusionsOPN levels in serum reflected the severity of the disease,which can be used to determine the effects of surgery and monitor recurrence and evaluate prognosis.

The detection of serum soluble B7-H4 levels in non-small cell lung cancer patients and its significance / 中华检验医学杂志

ObjectiveTo detect serum soluble B7-H4 (sB7-H4) levels in patients with non-small cell lung cancer ( NSCLC ) and explore its clinical significances.MethodsThe sB7-H4 levels the sera of in 102 NSCLC patients,60 benign lung disease patients and 90 healthy controls were determined by ELISA.The relation between serum sB7-H4 levels and NSCLC of pathological staging was analyzed by use of t test.ResultsThe serum sB7-H4 levels in NSCLC,benign lung disease and heathy control groups were (45.61 ±5.67 ),( 30.14 ± 3.51 ) and ( 28.52 ± 4.76 ) μg/L,respectively.The levels of NSCLC group were significantly higher than those of benign lung disease and healthy control groups ( t =2,59 and 3.61,P ＜ 0.05 ).The serum sB7-H4 levels of Ⅲ/IV stage NSCLC patients ( 63.93 t 4.76 ) μgL,with lymph node metastasis (61.73 ±3.97) μg/L and before operation patients (63.29 ±6.19) μg/L were relatively higher thanthose inthe Ⅰ/Ⅱ stage(35.12 ± 3.88 )μg/L, without lymphnode metastasis (40.26 ± 5.39 ) μg/L,after operation ( 40.51± 4.08 ) μg/L.There were significant difference ( t =6.78,5.73 and 3.67,P ＜ 0.05 ).Conclusions Serum sB7-H4 of NSCLC patients increases significantly and correlates with clinical stages and therapeutic conditions.Detecting on sB7-H4 is helpful for lung cancer diagnosis and therapeutic effect evaluation.

Expression and significance of histone deacetylase 6 in non-small cell lung cancer / 国际肿瘤学杂志

Objective To detect the expression of histone deacetylase 6 (HDAC6) and its significance in non-small cell lung cancer (NSCLC).Methods The expression of HDAC6 was measured by SP staining in 70 cases of NSCLC and 18 cases of normal lung tissues.Results Positive rate of HDAC6 immunostaining in NSCLC was 58.57％,which was significantly higher than 16.67％ in normal lung tissues(P ＜0.01 ).No correlation was found between HDAC6 expression and the age,sex,differentiated level,histological type ( P ＞0.05).But the expression level of HDAC6 was closely related to clinical stage and lymph node metastasis in NSCLC patients ( P ＜ 0.05 ).Conclusion HDAC6 over expression may be related to the pathogenesis and development of NSCLC.

The Clinical Research of Heart Rate Variability of 88 Diabetes Mellitus Cases with Hypertension and Body Posture Hypotension / 医学研究杂志

Objective To monitor the movement of blood pressure and the heart rate variability of 88 diabetes mellitus cases patients. Methods We analyzed the movement of blood pressure and the heart rate variability in 88 diabetes mellitus patients and 84 nonmal adults. Results (1) Diabetes mellitus patients can cause a function disorder of the heart the autonomic nerve. (2) The changes can cause a variation in blood pressure. Conclusion (1) The function confusion of the avfonomrc nerve appears earlier than clinical symptoms. (2)The variation in blood pressure of diabetes mellitus patients reflects damages to the the autonomic nerve.

The Clinical Research of Heart Rate Variability in 78 Cases of Myocardial Infarction / 医学研究杂志

Objective To study the heart rate variability of patients with myocardial infarction. Methods To analyze the time domain and frequency domain indexes indicators of the heart rate variability of 24h Holter in 78 cases of myocardial infarction patients and 76 cases of normal adults. Results (1) The time - domain analysis parameters of the myocardial infarction patients are reduced. (2) Myocardial infarction spectrum analysis showed LF was increased and HF was reduced. Conclusion (1) The regulation of the autonomic nervous imbalance appeared earlier than the clinical symptoms. (2) The reduction of HRV after myocardial infarction reflected a pathological state that the vagus nerve activity declined and the sympathetic nervous activity was dominant.

Experimental study of secondary osteoporosis of spinal cord injured rats / 中国组织工程研究

BACKGROUND: Osteoporosis is one of the complications of spinal cord injury, but its mechanism is unclear, different scholars have different points about whether all bones above and below the level of trauma are affected after spinal cord injury (SCI).OBJECTIVE: To observe the ultrastructure of bone tissue of secondary osteoporosis after spinal cord injury and the change of serum biochemical indexes, to analyze the suffered condition and injured degree of the bones above and below the level of the trauma.DESIGN: Randomized controlled experimentSETTING: Laboratory of Animals, and Department of Laboratory Medicine of the Second Clinical Hospital of Jilin University; Transmission Electron Microscope Center, Norman Bethune Division of Medical Sciences of Jilin UniversityMATERIALS: This experiment was conducted at the Laboratory of Animals, Second Clinical Hospital of Jilin University from May 2002 to May 2003. Totally 110 male Wistar rats, aged 4 to 5 month-old, with the body mass of (300 -320)g were involved.METHODS: Ten rats were randomly chosen from 110 male Wistar rats and were set as 0 week blank control group, the ther rats were randomly divided into experimental group and control group, there were 10 rats in week 1, 2, 3, 7 and 11 control group and experimental group separately.1 mL/L of pentobarbital natrium was intraperitoneal injected to perform anesthesia, in the control group, lamina with dura intact of rats were removed only, without spinal cord injury at the level of tenth thoracic vertebrae. In the experimental group, lamina of rats was also removed, the spinal cord injury model of the rats were made by the method of Allen's.4 mL venous blood was collected from the animals at the end of postoperative week 0, week 1, week 2, week 3, week 7 and week 11, then the animals were put to death, perform blood centrifuging .We observe the change of serum concentration of calcium, phosphorus and alkaline phophatase (ALP) with 7170A HITACHI auto-biochemistry analyzer. Right side humerus, tibia in week 0 blank control group, week 7 experimental group and week 11 experimental group were chosen, then 25 mL/L ethylene dinitrilotetraacetic acid was used for decalcium for 1 month for preparingthe sections. We observe the change of ultrastructure of osteocytes at the sites of tibial plateau and the surgical neck of the humerus after stained by uranyl acetate and lead citrate under transmission electron microscope.biochemistry detection: the level of phosphorus at week 2 of the experiment was significantly lower in the control group than experiment group [(1.54±0.21),(2.76±0.16)mmol/L, (P ＜ 0.01)]; the level of calcium at week 3 in the experiment group was significantly higher than in the control group [(2.52±0.06),(2.35±0.12)mmol/L, (P ＜ 0.01)];the level of alkaline phophatase at week 7 in the experiment group was significantly higher than in the control group [(155.86±20.42), (129.25±7.30)Nμ/mg,operative week 7, the tibia sample showed that the osteocyte separated from the osseous lacuna, the nucleus showed anomaly, fluffed materials appeared. Mitochondrion swelled, rough endoplasmic hollowed; At week 11, osteocyte separated from the osseous lacuna. At postoperative 7 week,the humerus sample showed that osteocyte separated from osseous lacuna,some nucleus pycnosis and rough endoplasmic hollowed; At postoperative week 11, the above changes still were seen, but the degree of cellular swelling and hollowing were lighten.CONCLUSION: At the early stage of spinal cord injury, the activity of osteoclast is increased and the activity of osteoblast is decreased, which leads to the increase of bone absorption, the bone formation is largely weakened. The bones above and below the level of trauma are both affected after spinal cord injury, but different extents of osteoporosis can be seen in different bones .The change of ultrastructure of osteocytes is remarkable when osteoporosis has happened.

The effects on morphology of middle turbinate and olfaction after endoscopic sinus surgery and conventional technique in sinus surgery / 中国耳鼻咽喉头颈外科

Objective: To observe the effects on morphology of middle turbinate and olfaction in trasnasal endoscopic sinus surgery and conventional technique in surgery in chronic sinusitis and nasal polyps (stage Ⅰ，Ⅱ)。 Methods: Two groups patients (85 cases, 163 sites) from September 1979 to September 1999 were examined by coronal CT scans of sinus, nasal endoscopy and olfctory test preoperative and postoperative. Of these patients, 52 cases (101 sites) were operated by transnasal endoscopic sinus surgery, 33 cases (62 sites) were operated by conventional technique in surgery respectively. Management of middle turbinate was divided into reserving and resecting two way. Results: In group of endoscopic sinus surgeries (52 cases, 101 sites), the rates of cure, improved and ineffective were 64.4% (65/101), 29.7%(30/101), 5.9%(6/101) respectively by following 6 months; and returning to normal rates of middle turbinate were 63.1% (41/65), 30.0% (9/30), 0.0% (0/6) respectively; and the returning to normal rates of olfaction were 66.2% (43/65), 36.7% (11/30),0.0%(0/6) respectively; the returning to normal of middle turbinate were 47.4%( 9/19),3.3%(1/13),0.0%(0/13) respectively; the returning to mormal rates of olfaction were 63.2%(12/19),23.3%(7/30),0.0%(0/13) respectively.Conclusion: Endoscopic sinus surgery for chronic sinusitis and nasal polyps can solve ostiomeatal complex anomalism more effectively and raise the cure rates of sinusitis (stage Ⅰ,Ⅱ) than conventional technique in surgery. The morphology middle turbinate and olfaction returned to normal affected directly sinusitis curative rate.

Characteristics of photosynthesis in three Dendrobium species / 中草药

Object To determine the diumal changes of photosynthesis, its relationships with light intensities and temperatures, and the contents of chloroophyll in leaves of Dendrobium candidum Wall. ex. Lindl., D. wilsonii Rolfe and D. hercoglossum Reichb. f. Methods The net photosynthetic rate (NPR) was measured at intervals of 1.5 h during daytimes 7:30—16:30, under the photosynthetic affective rates 0—140 mmol/(m 2?s), and under the temperatures 29.6—38.2 ℃; contents of chlorophyll a and b in leaves were measured. Results NPR, light saturation point and compensation point were low in each of the three Dendrobium L. species. The NPR peaked during 8:00 and 10:00 am, and thereafter decreased rapidly as the temperature and light intensity increased. Content of chlorophyll b was high in the leaves. Conclusion These results indicated that the Dendrobium L. species, which are sciophyte plants, have great adaptations to their growing habitats.