ABSTRACT
[Aim] The expressed system of sterol regulation by SCAP/SREBP Pathway was established in the oocyte of the Xenopus laevis and was applied to investigate the effect of cucurmin on the SREBP pathway.[Methods] The plasmid of PLXRN-4SRE-fpa was restructed and injected into the nucleus of Xenopus Oocytes in phase V or VI. The oocyte was incubated with different concentrations of the curcumin. After three days the fluorescence intensity on the membrane of the Oocytes was measured by Fluoroanalyzer.[Results] The fluoresecence intensity on the membrane of the oocyte increased with increasing of the concentration of the curcumin. The trail group which have all regulation elements of SCAP/SREBP pathway can express more fluorscence protein than the control group. [Conclusion] The experiment provides that the curcumin can upgrade the expression of low density lipoprotein receptor by SCAP/SREBP regulation pathway. The model can be applied for quick screening of the scap ligand drugs and inverstigating the mechanism of the drugs.
ABSTRACT
An issue that has to be settled urgently is how to accelerate hospital construction in a distressed county. Specific measures may include: ①clarifying the orientation of development on the basis of an analysis of the status quo; ②speeding up infrastructure construction while doing a good job of planning for the hospital area; ③raising funds from a variety of sources so as to purchase facilities urgently needed in medical treatment; ④taking vigorous steps for intensional building and personnel cultivation, such as formulating criteria for post assumption, with the backing of economic policies, selecting 12 to 15 people each year for higher training in advanced areas, and striving for support from big hospitals in the provincial capital; ⑤adopting integrated management and amplifying rules and regulations; ⑥striving for preferential policies and support from higher authorities: for instance, conformity between hospital construction and municipal planning alone saved over 3 million yuan of funds and the county government raised over 6 million yuan of funds for the hospital.