ABSTRACT
The metastasis of contralateral adrenal gland and gallbladder following radical nephrectomy is extremely uncommon in clinical practice. We presented one such case. The patient underwent laparoscopic radical right nephrectomy. Postoperative pathology revealed clear cell carcinoma of the right kidney. Five years after operation, CT revealed occupying lesions in the left adrenal gland and gallbladder. Transperitoneal laparoscopic left adrenalectomy and cholecystectomy were performed. Pathological examination showed that the left adrenal tumor and gallbladder tumor were clear cell carcinoma. The patient received targeted therapy and tumor-free survived for 10 months.
ABSTRACT
Objective:To investigate the functional mechanism of circular RNA signal-induced proliferation-associated gene 1(circSIPA1L1) on proliferation, migration and invasion of renal cell carcinoma cells, as well as to explore its mechanism.Methods:The study was completed between January 2019 and December 2019. Bioinformatics was used to analyze the expression of circular RNA(circRNA), circSIPA1L1 in renal cancer tissue and the information of circSIPA1L1. The expression of circSIPA1L1 mRNA, miR-22-3p in renal cancer tissues and renal cancer cells was detected by RT-qPCR. The circSIPA1L1 interference vector negative control (si-NC group), circSIPA1L1 interference vector (si-circSIPA1L1 group), si-circSIPA1L1+ miR-22-3p suppression vector plasmid negative control (anti-miR-NC group), si-circSIPA1L1 + miR-22-3p inhibition vector plasmid (anti-miR-22-3p group) were transfected into A498 and OSRC2 cells respectively. Dual luciferase reporter gene experiment was used to verify the targeting relationship. Clone formation experiment and Transwell chamber were used to detect cell proliferation, migration and invasion. The xenograft model was established by subcutaneous injection of A498/sh-circSIPA1L1 or A498/sh-NC (2×10 6 in 0.2 ml PBS/mice) on the right back of nude mice, and nude mice were divided into sh-circSIPA1L1 group and sh-NC group. Nude mice tumor formation experiments were used to detect tumor formation ability. Results:The expression of circSIPA1L1 mRNA in adjacent tissues and renal cancer tissues were (1.09±0.44) and (3.89±1.35) respectively. The expression of miR-22-3p were (1.02±0.30) and (0.44±0.19)respectively. The difference of the expression of circSIPA1L1 mRNA and miR-22-3p in kidney cancer tissue and adjacent tissues were statistically significant ( P<0.05). Compared with normal kidney cell KiMA, the expression of circSIPA1L1 mRNA in renal cancer cells A498 and OSRC2 was increased, and the expression of miR-22-3p was decreased ( P<0.05). The cell clone number of A498 and OSRC2 in the si-circSIPA1L1 group (130.67±15.04, 99.00±14.80) was lower than that in the si-NC group (314.33±29.57, 234.67±21.50), the number of cell migration (108.33±17.01, 85.67±11.93) was lower than si-NC group (265.00±20.00, 210.33±18.58), cell invasion number (84.00±12.00, 66.00±10.15) was lower than si-NC group (210.33±18.58, 173.00±17.52), and the differences were all statistically significant ( P< 0.05). CircSIPA1L1 targets and negatively regulates miR-22-3p expression. The cell clone number of A498 and OSRC2 in the si-circSIPA1L1+ anti-miR-22-3p group (234.20±21.90, 185.06±20.72) was higher than that in the si-circSIPA1L1+ anti-miR-NC group (134.65±26.55, 106.14±16.38), the migration cell number (187.02±23.54, 117.86 ±15.09) was higher than that of the si-circSIPA1L1+ anti-miR-NC group (110.59±12.12, 91.70±14.83), and the number of cell invasion (168.23±11.69, 103.70±9.23) was higher than that of the si-circSIPA1L1+ anti-miR-NC group (90.46±11.53, 61.35±9.10). The differences were all statistically significant ( P<0.05). The tumor volumes of nude mice in the sh-NC group and sh-circSIPA1L1 group on day 35 were (578.65±68.67) mm 3 and (242.56±42.35) mm 3 respectively, the tumor weights of nude mice were (0.68±0.06) g and (0.38±0.04) g respectively, the differences were statistically significant ( P<0.05). Conclusions:CircSIPA1L1 can promote the deterioration of renal cancer, promote the proliferation, migration, invasion of cancer cells and tumor growth. The mechanism of action is related to the direct targeting of miR-22-3p.
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Objective: To analyze chromosome aberrations in bladder transitional cell carcinoma with exfoliated cells, and to evaluate the clinical value of fluorescence in situ hybridization (FISH) in bladder cancer. Methods: FISH was performed using centromeric probes of 3, 7, 17 and locus probe of p16 to examine chromosome aberrations of exfoliated cells of 56 bladder cancer patients and 20 healthy controls to analyze the correlation of chromosome aberration with the pathological features of bladder cancer. The urine cytology of the 56 bladder cancer patients was performed. Results: The rates of aneuploidy of chromosomes 3, 7, and 17 were 58.9%, 39.3%, 58.9% and 75.0% for aberration of p16 in exfoliated cells from the 56 bladder cancer patients. All of the aberrations had no correlation with tumor stage (P>0.05). The aberrations of chromosomes 3, 7, and 17 were significantly correlated with pathological grade (P<0.05). The sensitivity of the 4 chromosome probes for detecting bladder cancer was 80.4%. The detection rate of FISH was obviously higher than that of udne cytology. Conclusion: Chromosome aberration is correlated with the growth of bladder cancer. The detection of FISH has significance for early di-agnosis, prognosis evaluation, and recurrence monitoring of bladder cancer.
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Objective To profile the subtypes of open reading frame 75(ORF75)of human herpesvirus 8(HHV-8)in patients with Kaposi's sarcoma,and to evaluate their relationship with clinical phenotypes and invasiveness of Kaposi's sarcoma.Methods Twenty-five paraffin-embeded tissue specimens of Kaposi's sarcoma were collected in the Department of Dermatology.People's Hospiml of Xinjiang Uygur Autonomous Region.DNA was extracted from these specimens.and nested-PCR was performed to amplify HHV-8 DNA followed bv bi-directional sequencing.Phylogenetic analysis was carried out by using the software DNASTAR,Clustal W program and PHYLIP package so as to identify the ORF75 subtyoe of HHV-8.Results HHV-8 DNA was detected in 21(84%)out of the 25 samples,and 7 cases of AIDS-associated Kaposi's sarcoma were all positive for HHV-8.Among the 21 patients carrying HHV-8 DNA,18 were positive for subtype A ORF75.3 for subtype C ORF75.The ORF75 subtypes had no significant correlation with the presence of mucosal lesions or clinical phenotypes of Kaposi's sarcoma.Conclusions The majority of patients with Kaposi's sarcoma in Xinjiang are infected with HHV-8 of ORF 75 subtype A and C.The ORF75 subtypes of HHV-8 have no correlation with the presence of mucosal lesions or clinical phenotypes of Kaposi's sarcoma.