ABSTRACT
Objective To investigate whether gemcitabine (GEM) could enhance radiosensitivity of human non-small cell lung cancer cells and its related mechanism.Methods Clonogenic assay was used to analyze radiosensitivity enhancement by GEM on p53 mutant human lung adenocarcinoma cell line 973.Alterations of cell cycle distribution and apoptosis were measured by flow cytometry.Results Mild radiosesitizing effect was observed when 10 nmol/L GEM was administrated before or after irradiation.Marked radiosesitizing effect was demonstrated when 100 nmol/L GEM was administrated before or after irradiation, with much stronger effect of pre-irradiation GEM treatment.Mutation of p53 gene affected cell cycle redistribution and cell apoptosis, but had no relationship with radiosensitivity enhancement of GEM.Conclusions 100 nmol/L GEM could significantly enhance radiosensitivity of human lung cancer cells.However, this effect may not be associated with p53 gene mutation, cell cycle redistribution or cell apoptosis.
ABSTRACT
Objective To study the effect of early postoperative enteral nutrition on patients after gastrectomy and nursing strategy. Methods Sixty patients with gastric cancer were assigned into the observation group and the control group after gastrectomy.Both groups accepted the regular nursing nutrition.The observation group also received early postoperative enteral nutritional supply 12 hours postoperation.The nutritional conditions, immune conditions, anal exsufflation and defecation and complications between two groups were recorded both preoperation and on the 8th day postoperation.χ2test and t test were used. Results On the 8th day postoperation,the observation group was better than the control group in prealbumin, serum albumin, transferrin, fibronectin and total protein,lymphocyte count, IgA, IgG and IgM.The observation group was also superior to the control group in recovery time of bowel sound, anal exsufflation and defecation, foodintake time, expense of nu-trition treatment, hospitalization time and incidence rate of complication. Conclusions The early postoperative enteral nutrition can promote nutritional condition, advance immunity, improve functional rehabilitation of gastrointestine and reduce the incidence rate of complication and hospitalization time.