ABSTRACT
AIM To investigate the improving effects of epigallocatechin gallate (EGCG) on rat kidney injury induced by cisplatin and its mechanism of action.METHODS Fifty male SD rats (10 rats/group) were randomly divided into blank control group,kidney injury group,EGCG low-,middle-and high-dose (25,50 and 100 mg/kg) groups.The kidney injury group and the drug administration group were treated with 7.5 mg/kg cisplatin by intraperitoneal injection to build the kidney injury model,and the blank control group was intraperitoneally injected with normal saline.After fourteen days of administration,the general condition and morphological changes of kidney tissue by HE staining were observed;BUN,Cr,Cys-c contents in serum,and IL-18,KIM-1 contents in urine were detected by ELISA;MDA,GSH and T-SOD contents in renal cortex were determined by kit;Western blot method was used to determine the contents of Nrf2 protein in renal contex cytoplasm and nucleus,and the expression level of HO-1 protein.RESULTS EGCG intervention could improve the pathological structural changes of rat kidney injury induced by cisplatin,decrease kidney index,and decrease serum Cr,Cys-c contents and urine IL-18,KIM-1 contents.Moreover,renal cortex MDA concentration decreased,and renal cortex GSH concentration,T-SOD activity increased.At the same time,renal cortex cytoplasm Nrf2 content reduced,but nucleus Nrf2 and total cell HO-1 contents increased.CONCLUSION EGCG plays a role in the improvement of rat kidney injury induced by cisplatin through the activation of Nrf2/HO-1 signal pathway.
ABSTRACT
OBJECTIVE:To explore a new way for the investigating of processing impact on the property of the traditional Chinese medicine(TCM)so as to provide new ideas and methods for the scientific research on TCM.METHODS:Ideas of in?vestigating the impact of processing on the property of TCM by using the method of biothermodynamics and setting up a new method system of research and evaluation on TCM were put forward through reviewing and analyzing the status quo and problems of processing impact on property of TCM and the commonness and characteristics between TCM and biothermody?natics.RESULTS&CONCLUSIONS:Theoretically and practically,it is feasible to investigate processing impact on the property of TCM by using biothermodynamic ideas and methods,and it is expected that which can serve as a new visual angle and technical platform in the modernization study of TCM.
ABSTRACT
Objective To establish a set of methods of Chinese materia medica quality evaluation based on biothermodynamics.Methods Using microcalorimetry,the thermal power of Escherichia coli meta-bolism affected by Rhizoma Coptidis in different growing years was measured.According to the thermokinetic model,the relationship of the thermokinetic parameters,caloric bioactivity and chemical constituent was analyzed.Results There were obvious difference in the caloric active fingerprints of E.coli metabolism affected by Rhizoma Coptidis in different growing years,which presented significant finger character.Compared with the normal groups,the metabolism of E.coli was inhibited in different levels by Rhizoma Coptidis in different growing periods,the multiplication rate constants and heat output were decreased,and the generation time was prolonged.Especially,Rhizoma Coptidis under four years' growing period with the highest content of total alkaloids showed a significant inhibitory effect on E.coli.Conclusion There are evident correlation among the thermokinetic parameters of E.coli metabolism affected by Rhizoma Coptidis in different growing years,the caloric bioactivity,and chemical composition.The thermokinetic parameters can indirectly reflect caloric bioactivity of Rhizoma Coptidis.As the method of biothermodynamics is on line,microamount,high throughout screening,and universality.The microcalorimetry is considered to be a useful mean for Chinese materia medica quality evaluation.