Clinicopathological features of follicular lymphoma in children / 中华病理学杂志

Objective@#To investigate the clinicopathologic features of follicular lymphoma (FL) in children.@*Methods@#One female and one male patients with FL diagnosed in the First College of Clinical Medical Science, China Three Gorges University and Beijing Friendship Hospital of the Capital University of Medical Science in February 2016 and June 2015 were studied by HE immunohistochemistry, EBER in situ hybridization, IgH and IgK gene rearrangement analysis and IRF4 fusion gene detection.@*Results@#The two patients′ age were 6.3 and 12 years, respectively. The lesions involved head and neck lymph nodes with duration of more than 2 months. Histopathologically, the lesions consisted of nodular proliferation of lymphoid follicles with diffuse distribution of large cells. Starry sky phenomenon was seen in one of the two cases. Immunohistochemistry showed that one case was positive for bcl-2 and MUM1, but negative for bcl-6 and CD10. Ki-67 index was>50% and oligoclonal IgK rearrangement was observed. The second case showed positivity for bcl-6, and CD10 but negative for bcl-2. Ki-67 index was>50% and clonal IgH FR1-JH and IgH FR2-JH rearrangements were detected. Both cases showed no evidence of IRF4 gene fusion.@*Conclusions@#Childhood FL is a rare B-cell lymphoma with characteristic features and high-grade histomorphology. However, its immunophenotype and molecular genetic characteristics are divergent.

Effect of combined use of PDTC and paclitaxel on proliferation and invasion of human breast cancer cell line MCF-7 / 生物医学工程学杂志

This investigation was made with special reference to the effect of the combined use of nuclear factor-kappaB (NF-kappaB) inhibitor Pyrrolidine dithiocarbamate(PDTC) and Paclitaxel on the expression of Matrix metalloproteinases (MMP-9) and its inhibitor TIMP-1, and on the proliferation and invasion of human breast cancer cell line MCF-7. The MCF-7 cells were treated with PDTC and Paclitaxel. The effect on proliferation was evaluated by MTT assay. The cell cycle was analyzed by flow cytometry. Western blot was used to determine the change of NF-kappaB p65, MMP-9 and TIMP-1 expression in MCF-7 cells after treatment. RT-PCR was used to detect NF-kappaB p65 mRNA expression. The invasion ability of MCF-7 cells was tested by the invasion, migration and cell adhesion assay. The cell growth was significantly slowed down and the cell cycle was arrested at G0/G1 phase after the combined treatment. The expression of NF-kappaB p65 and MMP-9 was down-regulated and the invasion ability of MCF-7 cells was decreased after the combined treatment. In conclusion,PDTC combined with paclitaxel effectively inhibited cell proliferation, induced cell cycle arrest, and decreased cell invasion ability of breast cancer MCF-7 cells. The mechanism may be associated with the inhibiting effect of PDTC on the NF-kappaB-related gene expression.

The preparation of bioglass/collagen/phosphoserine biomemetic composite scaffold and a study on its cytocompatibility / 生物医学工程学杂志

In the present study, novel biomemetic composite scaffolds using the sol-gel derived bioactive glass (BG), collagen (COL), and phosphoserine (PS) were prepared by freeze-drying. MC3T3-E1 were cultivated in vitro, collected and seeded onto the surface of BG, BG-COL and BG-COL-PS. Cell attachment and proliferation were observed. The cell proliferation was tracked by MTT method 1,3,5 d after seeding. MTT showed that the cells can adhere to and proliferate well on the surface of the scaffolds, and the cell proliferation result of scaffold BG-COL-PS was better than those of scaffolds BG and BG-COL. Therefore, the scaffold BG-COL-PS can be a potential scaffold for tissue engineer.

Isolation and cultivation of goat embryo stem cells / 生物工程学报

Morulaes and blastocysts obtained from Guanzhong dairy goats 6-7 days after mating were treated with whole embryo cultivaton, enzymatic digestion and immunosurgery separately. The goat embryonic stem cells (ESC) were isolated and cultured on a feeder layer of mitomycin-inactivated mouse embryo fibroblasts (MEF). The characteristics of goat ESCs were analyzed by immunohistochemisty, RT-PCR and inducing differentiation in vitro. The results indicated that the embryos were easier to attach the culture dish and form primary colonies with whole embryo method. There were colonies that maintained undifferentiated for 18 passages. The ESCs expressed the protein of Nanog, Oct4 and SSEA-3, whereas the protein of SSEA-4 was absent and the protein of SSEA-1 was weakly expressed. In addition, the genes of Nanog, Oct4, TERT and CD117 were expressed in goat ESCs. The cells also could differentiate to myocardial cells when induced in vitro by DMSO. These results suggest that the goat ESCs have characteristics of ESCs.

Research process and problem of bone tissue engineering scaffold / 中国组织工程研究

Based on the requirements of ideal bone tissue engineering scaffold need to meet, this paper briefly summarized bone tissue engineering scaffold from material selection, design and preparation. Many domestic and abroad experiments have confirmed that bone tissue engineering scaffold is feasible, effective and superior in repairing bone tissue defects. However, so far, the constructed bone tissue engineering scaffold is far from perfect, some areas, such as the mechanical properties, degradation of speed, structure and biological activity, are still need to be resolved. With further carrying out, the research of bone tissue engineering scaffold will have ground-breaking progress, and it will have a bright future in the clinical applications.

Influence of crosslinking on the biological and physical properties of collagen / 中国组织工程研究

BACKGROUND:Water-soluble 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide(EDC) and N-hydroxysuccinimide(NHS) are non-toxic and good biocompatible crosslinking agents which can trigger collagen to crosslink and form amide bond.OBJECTIVE:To analysis the influence of crosslinking on the biological and physical properties of collagen.DESIGN,TIME AND SETTING:A crosslinking experiment of collagen was performed at the Laboratory in Department of Materials Science and Engineering of Fujian University of Technology from September to November 2008.MATERIALS:Ⅰ-type bovine collagen solution was provided by Guangzhou Chuang'er Company.EDC and NHS,as the crosslinking agents,were provided by Shanghai Gill Biochemical Company and Shanghai Bo'ao Biological Technology Limited Company,respectively.2-(N-Morpholino) ethane sulfonic acid(MES),as a buffer,was provided by Shanghai Juyuan Biological Technology Limited Company.METHODS:Collagen solution was freezed at -80 ℃ for 12 hours in refrigerator.After vacuum freeze-drying 24 hours for complete dehydration in freeze-drying equipment,collagen matrices were obtained.The collagen matrices were immersed into different concentration of EDC.NHS was added according to EDC:NHS=4:1.The MES was as the buffer to adjust pH to 3.0.Then the solution was treated as follow:washed by deionized water,vacuum freeze-drying for complete dehydration.MAIN OUTCOME MEASURES:The microstructure,shrinkage temperature,characteristics of swelling dynamics and ability of antidegradation of collagen before and after crosslinking were analyzed.RESULTS:The studies of collagen crosslinking showed that the free -NH2 functional group of collagen molecules changed into N-H functional group after crosslinking,and the amino-link formed between collagen molecules.When the concentration of EDC reached 2 g/L,the crosslinking achieved to the greatest degree.After crosslinking,the microstructure of collagen changed from a state of disorder into the close and orderly structure,and the thermal stability and shape stability enhanced.In addition,the ability to antidegradation also significantly increased after crosslinking.CONCLUSION:Using EDC and NHS as crosslinking agents,crosslinking treatment can effectively improve the biological and physiochemical properties of collagen as bone tissue engineering material.

Analysis of four kinds of autoimmune antibodies in sera of patients with viral hepatitic disease / 中国免疫学杂志

Objective:To analyse clinical signification of four kinds of autoimmune antibodies in the patients with viral hepatitis.Methods:ANA RF,anti TM and dsDNA Ab in sera of patients with viral hepatitic disease were detected by means of indirect immune fluerscence(IIF) and ELISA.Results:12 of 63 (19.1%) were only one kind of autoimmune antibody in sera of patients with hepatitis B and 12 of 40(30%) of patients with hepatitis C.There were significantly higher than those of alcoholic and drug hepatitis group(P