1.
Chinese Journal of Cancer Biotherapy
;
(6): 282-284, 2000.
Article
in Chinese
| WPRIM
| ID: wpr-412396
ABSTRACT
Objective: To construct the recombinant vaccinia virus of mutant N-ras/61 gene and enhance the immunogenecity of mutant N-ras/61 protein produced by the recombinant vaccinia virus. Methods: N-ras/61 gene was inserted into P1108 and transfected into CV-1 cell infected with vaccinia virus by Cell FECTIN. PCR and Western blot were used to identify the recombinants. Results: We get recombinant vaccinia virus rV-N-ras/61 by PCR and tk- selecting. The expression of N-ras gene was detected by Western blot. Conclusion: This study is a test for studing effective vaccine of mutant N-ras/61 gene. The efficacy in vivo of the N-ras/61 vaccine in immunotherapy is under investgation.